##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064039_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1498673
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2799142975152	32.0	32.0	32.0	32.0	32.0
2	30.908238154687513	32.0	32.0	32.0	32.0	32.0
3	30.927850838708643	32.0	32.0	32.0	32.0	32.0
4	30.95665098390376	32.0	32.0	32.0	32.0	32.0
5	30.870113760640248	32.0	32.0	32.0	32.0	32.0
6	34.51824847715279	36.0	36.0	36.0	32.0	36.0
7	34.46095512496722	36.0	36.0	36.0	32.0	36.0
8	34.422306266944155	36.0	36.0	36.0	32.0	36.0
9	34.56086684687053	36.0	36.0	36.0	32.0	36.0
10	34.28458843256668	36.0	36.0	36.0	32.0	36.0
11	34.524679499797486	36.0	36.0	36.0	32.0	36.0
12	34.358541856695894	36.0	36.0	36.0	32.0	36.0
13	34.436669640408546	36.0	36.0	36.0	32.0	36.0
14	34.349218275100704	36.0	36.0	36.0	32.0	36.0
15	34.283700313544045	36.0	36.0	36.0	32.0	36.0
16	34.27483780651283	36.0	36.0	36.0	32.0	36.0
17	34.21072708989887	36.0	36.0	36.0	32.0	36.0
18	34.19458080581955	36.0	36.0	36.0	32.0	36.0
19	34.21249131731872	36.0	36.0	36.0	32.0	36.0
20	34.18764934044985	36.0	36.0	36.0	32.0	36.0
21	34.172067555764336	36.0	36.0	36.0	32.0	36.0
22	34.15534676343672	36.0	36.0	36.0	32.0	36.0
23	34.10499888901715	36.0	36.0	36.0	32.0	36.0
24	34.080909578006676	36.0	36.0	36.0	32.0	36.0
25	33.684796483288885	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	17.0
5	19.0
6	56.0
7	28.0
8	80.0
9	117.0
10	121.0
11	36.0
12	88.0
13	71.0
14	153.0
15	271.0
16	445.0
17	537.0
18	715.0
19	963.0
20	1418.0
21	1994.0
22	3180.0
23	4770.0
24	7116.0
25	10572.0
26	15157.0
27	20320.0
28	28021.0
29	38686.0
30	51569.0
31	70913.0
32	101510.0
33	145089.0
34	313723.0
35	680912.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.749741841214494	16.850709794814662	10.979374664994337	26.420173698976512
2	16.945437220239636	19.537115875841785	36.94291487277493	26.57453203114365
3	19.037972250970377	23.447749870383085	28.259823694305595	29.254454184340943
4	12.688931016282215	15.156853363974673	35.423977515449124	36.730238104293996
5	14.841969622452773	36.21228915324973	33.53475146826146	15.410989756036036
6	34.737107636784906	35.03420790612379	16.675386590714346	13.553297866376951
7	30.509571824052493	30.06021265974174	20.756282218644966	18.673933297560794
8	28.45505608602639	32.67994753511843	19.22230491708079	19.642691461774394
9	27.656757877197236	13.783985318729503	18.23309387481533	40.32616292925793
10	16.073934611199018	26.35147315080017	31.370667841715573	26.203924396285245
11	37.727916980169695	20.643264819986392	22.30971005949158	19.319108140352327
12	25.17840164941405	23.279278701834226	28.515420525804142	23.02689912294758
13	29.860454557594213	19.078532703041954	24.958002830969743	26.103009908394093
14	23.761302617012383	19.457527799997596	24.721399087377527	32.0597704956125
15	25.28937924588139	27.055398841615062	21.88112366677238	25.77409824573117
16	25.934210333034653	25.276794406289188	23.636993700932507	25.15200155974366
17	24.37959859521679	25.472445150693712	24.858186334076677	25.289769920012816
18	25.154203819486874	24.506738090897617	25.89742716511378	24.44163092450173
19	26.03577242721247	24.462765879306453	24.90554749068162	24.595914202799456
20	26.140672349939297	24.05432008408715	24.433620435207086	25.371387130766465
21	27.07173945850511	23.941243010287412	23.994195884232862	24.99282164697461
22	26.416215696435152	24.08401401791772	24.359270576796714	25.14049970885041
23	24.683533314813232	23.945422685699633	25.202942024959395	26.168101974527737
24	25.261428743138747	24.501983252534156	24.942105052285747	25.294482952041346
25	25.156407446657198	24.202103328745782	25.017578934926888	25.623910289670132
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	85.0
1	85.0
2	266.0
3	447.0
4	447.0
5	447.0
6	1194.5
7	1942.0
8	1942.0
9	1942.0
10	2192.5
11	2443.0
12	2443.0
13	2443.0
14	2913.5
15	3384.0
16	3384.0
17	3384.0
18	5746.5
19	8109.0
20	8109.0
21	8109.0
22	13525.0
23	18941.0
24	18941.0
25	18941.0
26	28642.0
27	38343.0
28	38343.0
29	38343.0
30	48530.5
31	58718.0
32	58718.0
33	58718.0
34	73777.5
35	88837.0
36	88837.0
37	88837.0
38	102570.0
39	116303.0
40	116303.0
41	116303.0
42	135331.5
43	154360.0
44	154360.0
45	154360.0
46	173436.0
47	192512.0
48	192512.0
49	192512.0
50	198546.5
51	204581.0
52	204581.0
53	204581.0
54	192432.5
55	180284.0
56	180284.0
57	180284.0
58	167005.0
59	153726.0
60	153726.0
61	153726.0
62	135691.5
63	117657.0
64	117657.0
65	117657.0
66	97172.0
67	76687.0
68	76687.0
69	76687.0
70	57479.5
71	38272.0
72	38272.0
73	38272.0
74	29442.0
75	20612.0
76	20612.0
77	20612.0
78	16782.0
79	12952.0
80	12952.0
81	12952.0
82	8793.5
83	4635.0
84	4635.0
85	4635.0
86	3604.5
87	2574.0
88	2574.0
89	2574.0
90	1832.0
91	1090.0
92	1090.0
93	1090.0
94	749.0
95	408.0
96	408.0
97	408.0
98	589.5
99	771.0
100	771.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03763329291980305
2	0.0046040730699759055
3	0.0017348681133242541
4	0.0029359306533179685
5	0.008807791959953905
6	0.012611156669934002
7	0.02115204584322264
8	0.03523116783981562
9	0.0473752446330854
10	0.05758427622303198
11	0.05431471708638242
12	0.06198817220300893
13	0.06358958892300055
14	0.06745967932964696
15	0.059919675606353084
16	0.06612516539632061
17	0.0622550749896742
18	0.07620074559293455
19	0.07306463784961763
20	0.0786695963695883
21	0.07439915178294397
22	0.07746853382959458
23	0.08013756169624728
24	0.07560021432293769
25	0.07620074559293455
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1498673.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.006866962868656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.19319812764749	40.145763854629244
2	13.407027725816775	13.945150146080893
3	4.0720427155281484	6.353225513207726
4	1.7666702198345083	3.675159323607808
5	0.9351124619943559	2.431613470313052
6	0.5536182152521049	1.72751693213022
7	0.3674102185157147	1.3375498048601695
8	0.275364288799874	1.1456667147151993
9	0.20520978437859067	0.9605086160090694
>10	1.0401750644013763	9.779929585205602
>50	0.08652104861517632	3.1184435656818845
>100	0.08346063605025547	9.039801803071668
>500	0.010577622178039921	3.728330132405828
>1k	0.003611870987623388	2.6113405380818144
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3122	0.20831762499224313	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2138	0.14265953947258675	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1654	0.1103643022860891	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1596	0.10649421187944266	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1560	0.10409208679945527	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1533	0.10229049298946467	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1526	0.10182341311280045	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3345139333263494E-4
2	0.0	0.0	0.0	0.0	1.3345139333263494E-4
3	0.0	0.0	0.0	0.0	2.0017708999895241E-4
4	0.0	0.0	0.0	0.0	2.669027866652699E-4
5	0.0	0.0	0.0	6.672569666631747E-5	2.669027866652699E-4
6	0.0	0.0	0.0	6.672569666631747E-5	4.0035417999790483E-4
7	0.0	0.0	0.0	6.672569666631747E-5	4.0035417999790483E-4
8	0.0	0.0	0.0	6.672569666631747E-5	4.670798766642223E-4
9	0.0	0.0	0.0	6.672569666631747E-5	5.338055733305398E-4
10	0.0	0.0	0.0	1.3345139333263494E-4	5.338055733305398E-4
11	0.0	0.0	0.0	1.3345139333263494E-4	5.338055733305398E-4
12	0.0	0.0	0.0	1.3345139333263494E-4	8.007083599958097E-4
13	0.0	0.0	0.0	1.3345139333263494E-4	0.0010676111466610795
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCGAC	25	0.0060287328	19.00177	7
TCGGTTG	35	0.0021673494	16.288862	17
TATAGCG	40	0.0052850815	14.247521	5
GCGTTAT	120	3.6379788E-12	14.245143	1
CGTTATT	120	3.6379788E-12	14.243717	2
AGGCCCG	325	0.0	13.446958	10
CGCGGTA	85	2.6946327E-7	13.413463	18
ATCGCCA	250	0.0	13.302572	16
CGACCAT	310	0.0	13.178207	10
CGAGCCG	335	0.0	12.763661	15
CGATAAC	150	1.8189894E-12	12.667423	10
CCGACCA	325	0.0	12.570402	9
CGCATCG	245	0.0	12.409733	13
CGTCGTA	215	0.0	12.372832	10
CGGTCCA	300	0.0	12.350739	10
TATTAGC	155	1.8189894E-12	12.25266	2
CGAACGA	140	5.2750693E-11	12.216647	16
TATTCCC	220	0.0	12.088806	5
AATACTA	80	2.8713052E-5	11.872934	5
GTTATTC	160	3.6379788E-12	11.869764	3
>>END_MODULE