FastQCFastQC Report
Thu 2 Feb 2017
SRR4064039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498673
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16390.10936341683609432No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15850.10576022921611318No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15270.10189013880946678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25450.022.9061341
CTAGCGG4850.021.77537529
TAGCGGC4950.021.33689530
CGCAATA5100.020.70864736
TCTAGCG5150.020.50690728
TACACCG654.935143E-420.3063375
AAACCGT550.00448583719.9986656
ATACGAA5550.018.62876540
CACGATT600.007405780718.33639330
TAGGACG4600.018.17274
GCGCAAT5850.018.05369235
AACGCAG32100.018.0237186
GTATAGT1355.5324563E-817.924731
CAATACG5900.017.89890338
AATACGA5900.017.89651539
TACGAAT5750.017.59823841
CGCCGGT6050.017.453387
GCAATAC6200.017.38825837
CGGTCCA6450.017.05426410
GTAGGAC6150.016.8113253
Produced by FastQC (version 0.11.5)