FastQCFastQC Report
Thu 2 Feb 2017
SRR4064038_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064038_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691416
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15040.2175246161500457No Hit
TATCAACGCAGAGTACTTTTTTTTT12780.18483807143600958No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12130.1754370740625036No Hit
GAGTACTTTTTTTTTTTTTTTTTTT8370.12105592002499219No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG8000.11570458305853494No Hit
GATTAAGAGGGACGGCCGGGGGCAT7830.11324586066854107No Hit
GAACTACGACGGTATCTGATCGTCT7740.11194418410913257No Hit
CTATTGGAGCTGGAATTACCGCGGC7480.10818378515973018No Hit
GTATCTGATCGTCTTCGAACCTCCG7480.10818378515973018No Hit
GCGCAAGACGGACCAGAGCGAAAGC7430.10746063151561433No Hit
GTCCTATTCCATTATTCCTAGCTGC7400.10702673932914482No Hit
GAATAGGACCGCGGTTCTATTTTGT6940.10037372580327907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAA551.124791E-515.54794213
AACGTCT902.2810127E-914.7769346
TCCTATA456.791993E-414.7673122
GATAACG653.3574634E-614.61878211
ATTCCGA653.3675715E-614.614556
TAAATCT400.005287322714.245064
TAGGACG801.2903365E-714.245064
CTAAATC400.005292402614.2429993
ATTCGAA1006.6211214E-1014.2419691
CAATTAG400.00529494414.2419691
GAACGTC954.8912625E-913.9981865
CGATAAC759.632931E-713.93556310
CCGATAA707.236604E-613.5736019
TTCCGAT707.252862E-613.5706537
CGAACGT1001.0082658E-813.2953914
TCGAACG1001.0104486E-813.2934663
TCCCCGC500.001504455213.2925041
CATTATA500.001506096813.2905822
ACGTCTG1153.765308E-1013.2166357
TATAAGA655.4802527E-513.144532
Produced by FastQC (version 0.11.5)