##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064038_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	691416
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16116780635681	32.0	32.0	32.0	32.0	32.0
2	30.848317076839415	32.0	32.0	32.0	32.0	32.0
3	30.859285871313364	32.0	32.0	32.0	32.0	32.0
4	30.896758246844158	32.0	32.0	32.0	32.0	32.0
5	30.809048387656635	32.0	32.0	32.0	32.0	32.0
6	34.40037546137202	36.0	36.0	36.0	32.0	36.0
7	34.34102190287757	36.0	36.0	36.0	32.0	36.0
8	34.29822133129693	36.0	36.0	36.0	32.0	36.0
9	34.42480648408483	36.0	36.0	36.0	32.0	36.0
10	34.16806235319981	36.0	36.0	36.0	32.0	36.0
11	34.416347032755965	36.0	36.0	36.0	32.0	36.0
12	34.251732676131304	36.0	36.0	36.0	32.0	36.0
13	34.322150485380725	36.0	36.0	36.0	32.0	36.0
14	34.22981244287086	36.0	36.0	36.0	32.0	36.0
15	34.16889397989054	36.0	36.0	36.0	32.0	36.0
16	34.165550117440155	36.0	36.0	36.0	32.0	36.0
17	34.09525524431023	36.0	36.0	36.0	32.0	36.0
18	34.08922269661101	36.0	36.0	36.0	32.0	36.0
19	34.08739601050598	36.0	36.0	36.0	32.0	36.0
20	34.08458005021579	36.0	36.0	36.0	32.0	36.0
21	34.0541367281058	36.0	36.0	36.0	32.0	36.0
22	34.0270748724357	36.0	36.0	36.0	32.0	36.0
23	33.97689957999236	36.0	36.0	36.0	32.0	36.0
24	33.96360801601352	36.0	36.0	36.0	32.0	36.0
25	33.57535405602416	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	7.0
5	7.0
6	27.0
7	17.0
8	35.0
9	40.0
10	65.0
11	17.0
12	38.0
13	31.0
14	78.0
15	147.0
16	199.0
17	320.0
18	346.0
19	479.0
20	743.0
21	963.0
22	1549.0
23	2361.0
24	3568.0
25	4985.0
26	7426.0
27	10021.0
28	13899.0
29	19235.0
30	25764.0
31	35435.0
32	50619.0
33	71634.0
34	148805.0
35	292551.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03517366908682	17.387165010952888	11.223598044974839	26.354063274985457
2	16.608328897734374	20.060574853625234	37.412927263891135	25.91816898474925
3	18.555250216951112	24.128724327451547	28.902227364767143	28.4137980908302
4	12.331804513561947	15.355888125684316	36.35134216490715	35.96096519584658
5	14.350349823316444	36.890195834520625	33.78235883757697	14.97709550458596
6	33.68782374613241	35.86018651248841	17.120122200830004	13.33186754054918
7	29.81578779865407	30.69576488424171	21.28494303222484	18.203504284879383
8	28.04703901060664	32.67932248314816	19.84328046170919	19.43035804453601
9	27.559627080237366	13.815626686654504	18.684622920124383	39.94012331298375
10	15.692667474172126	26.666493009578634	31.993134756453166	25.64770475979607
11	37.18801009481252	20.897101214387423	22.853839385976084	19.061049304823978
12	24.48874449163887	23.68598904478325	29.268229147823067	22.557037315754815
13	29.325728378051764	19.34183656486042	25.46859193027601	25.86384312681181
14	23.473735397297705	19.717700385546074	25.490760787764412	31.31780342939181
15	24.945730196586936	27.27325352536643	22.607352907124827	25.173663370921805
16	25.56474873764601	25.574445237209666	24.0254656128839	24.83534041226043
17	23.76073991638169	25.6733386686194	25.63817209047217	24.927749324526737
18	24.625763887481654	24.811615432046942	26.522347056047845	24.040273624423556
19	25.485521908913807	24.85461119168165	25.54993009179252	24.109936807612034
20	25.623314607961344	24.310029629608184	25.041288938617352	25.025366823813116
21	26.819867361796607	24.238893504433335	24.436171844921567	24.505067288848494
22	25.703744231960584	24.205211933834725	25.093214990823242	24.997828843381445
23	24.34600597540357	24.110637607985733	25.507214489195633	26.03614192741506
24	24.79460739471277	25.0026054030269	25.251131902870576	24.951655299389756
25	24.83737801541833	24.46089400838947	25.48540527396051	25.216322702231693
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	145.0
1	145.0
2	264.5
3	384.0
4	384.0
5	384.0
6	1027.0
7	1670.0
8	1670.0
9	1670.0
10	1713.0
11	1756.0
12	1756.0
13	1756.0
14	1839.0
15	1922.0
16	1922.0
17	1922.0
18	3240.0
19	4558.0
20	4558.0
21	4558.0
22	7573.0
23	10588.0
24	10588.0
25	10588.0
26	15667.5
27	20747.0
28	20747.0
29	20747.0
30	25391.0
31	30035.0
32	30035.0
33	30035.0
34	37140.5
35	44246.0
36	44246.0
37	44246.0
38	50216.0
39	56186.0
40	56186.0
41	56186.0
42	64389.5
43	72593.0
44	72593.0
45	72593.0
46	81316.5
47	90040.0
48	90040.0
49	90040.0
50	91922.0
51	93804.0
52	93804.0
53	93804.0
54	87008.0
55	80212.0
56	80212.0
57	80212.0
58	73240.5
59	66269.0
60	66269.0
61	66269.0
62	57983.5
63	49698.0
64	49698.0
65	49698.0
66	40785.5
67	31873.0
68	31873.0
69	31873.0
70	24026.5
71	16180.0
72	16180.0
73	16180.0
74	12478.5
75	8777.0
76	8777.0
77	8777.0
78	7128.0
79	5479.0
80	5479.0
81	5479.0
82	3783.5
83	2088.0
84	2088.0
85	2088.0
86	1610.5
87	1133.0
88	1133.0
89	1133.0
90	820.5
91	508.0
92	508.0
93	508.0
94	333.0
95	158.0
96	158.0
97	158.0
98	262.5
99	367.0
100	367.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03962881969754822
2	0.005785229152926748
3	0.002314091661170699
4	0.004772814051164567
5	0.009690258831152302
6	0.013739919238201025
7	0.021405347865828966
8	0.03543452856167632
9	0.0475835097828225
10	0.05770766080044431
11	0.05322410820692608
12	0.06088953683455402
13	0.06696402744512711
14	0.06479456651277958
15	0.06132342902102352
16	0.06436067432631006
17	0.06060027537690768
18	0.07838985502215742
19	0.07376167169981603
20	0.07998079303921228
21	0.0743401946151087
22	0.0781005935645111
23	0.08446434563273052
24	0.07867911647980376
25	0.07954690085274278
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	691416.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.8628429626108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4919083239549	47.96845402396433
2	11.70784588292164	13.783141872737318
3	3.1473924824753543	5.557934083129455
4	1.205176815098757	2.837605344373502
5	0.6192656283656286	1.8225867717314244
6	0.3716694927276141	1.3126513790651257
7	0.2546146351195357	1.0491138898116572
8	0.175785933338085	0.8277807831292533
9	0.12783547207109908	0.677228338581509
>10	0.7224325720218118	7.959305605312385
>50	0.08359765550014663	3.4933279057264044
>100	0.08483049760937766	9.517294215644146
>500	0.00690480794485991	2.6136778154176588
>1k	7.398008512349904E-4	0.5798979713758495
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1504	0.2175246161500457	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1278	0.18483807143600958	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1213	0.1754370740625036	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	837	0.12105592002499219	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	800	0.11570458305853494	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	783	0.11324586066854107	No Hit
GAACTACGACGGTATCTGATCGTCT	774	0.11194418410913257	No Hit
CTATTGGAGCTGGAATTACCGCGGC	748	0.10818378515973018	No Hit
GTATCTGATCGTCTTCGAACCTCCG	748	0.10818378515973018	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	743	0.10746063151561433	No Hit
GTCCTATTCCATTATTCCTAGCTGC	740	0.10702673932914482	No Hit
GAATAGGACCGCGGTTCTATTTTGT	694	0.10037372580327907	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4463072882316868E-4
2	0.0	0.0	0.0	0.0	1.4463072882316868E-4
3	0.0	0.0	0.0	0.0	1.4463072882316868E-4
4	0.0	0.0	0.0	0.0	1.4463072882316868E-4
5	0.0	0.0	0.0	0.0	1.4463072882316868E-4
6	0.0	0.0	0.0	0.0	1.4463072882316868E-4
7	0.0	0.0	0.0	0.0	1.4463072882316868E-4
8	0.0	0.0	0.0	0.0	1.4463072882316868E-4
9	0.0	0.0	0.0	0.0	1.4463072882316868E-4
10	0.0	0.0	0.0	0.0	1.4463072882316868E-4
11	0.0	0.0	0.0	0.0	1.4463072882316868E-4
12	0.0	0.0	0.0	0.0	2.8926145764633735E-4
13	0.0	0.0	0.0	0.0	2.8926145764633735E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGAA	55	1.124791E-5	15.547942	13
AACGTCT	90	2.2810127E-9	14.776934	6
TCCTATA	45	6.791993E-4	14.767312	2
GATAACG	65	3.3574634E-6	14.618782	11
ATTCCGA	65	3.3675715E-6	14.61455	6
TAAATCT	40	0.0052873227	14.24506	4
TAGGACG	80	1.2903365E-7	14.24506	4
CTAAATC	40	0.0052924026	14.242999	3
ATTCGAA	100	6.6211214E-10	14.241969	1
CAATTAG	40	0.005294944	14.241969	1
GAACGTC	95	4.8912625E-9	13.998186	5
CGATAAC	75	9.632931E-7	13.935563	10
CCGATAA	70	7.236604E-6	13.573601	9
TTCCGAT	70	7.252862E-6	13.570653	7
CGAACGT	100	1.0082658E-8	13.295391	4
TCGAACG	100	1.0104486E-8	13.293466	3
TCCCCGC	50	0.0015044552	13.292504	1
CATTATA	50	0.0015060968	13.290582	2
ACGTCTG	115	3.765308E-10	13.216635	7
TATAAGA	65	5.4802527E-5	13.14453	2
>>END_MODULE