FastQCFastQC Report
Thu 2 Feb 2017
SRR4064038_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064038_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691416
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8470.12250222731322387No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7810.11295659921089474No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7740.11194418410913257No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7510.10861767734619968No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7390.10688210860032167No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7340.10615895495620581No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA7340.10615895495620581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG1004.6182322E-821.9993865
GTATCAA12900.020.635081
ACTAGGC550.0044824319.999443
GTCCTAA1252.2844688E-819.359461
GCGGGGA1605.2750693E-1119.25224736
CGTCGTA2450.018.85661510
TACCGTC2600.018.6148667
ATACCGT2850.018.5257996
GGTCCTA2400.018.33281913
AACGCAG14550.018.1438246
ACCGTCG2550.018.117148
CGCAATA1951.8189894E-1218.05338936
CCGTCGT2750.017.5995089
TAGGGTG750.001290354117.5995085
GTATAGG1002.4811181E-517.5995081
CGGCTCG2650.017.43599736
AATACGA2109.094947E-1216.7626539
GTTATAG1053.771479E-516.7614361
GAATATA800.001987562316.499541
CTTATAG800.001987562316.499543
Produced by FastQC (version 0.11.5)