##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064038_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	691416
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.178021625186574	32.0	32.0	32.0	32.0	32.0
2	31.139277945549424	32.0	32.0	32.0	32.0	32.0
3	31.302693313432144	32.0	32.0	32.0	32.0	32.0
4	31.434266780057158	32.0	32.0	32.0	32.0	32.0
5	31.320390329410948	32.0	32.0	32.0	32.0	32.0
6	34.91393748481377	36.0	36.0	36.0	36.0	36.0
7	34.93742262256008	36.0	36.0	36.0	36.0	36.0
8	34.864683490113045	36.0	36.0	36.0	32.0	36.0
9	35.02847923681257	36.0	36.0	36.0	36.0	36.0
10	34.80106911034746	36.0	36.0	36.0	32.0	36.0
11	35.03756638550453	36.0	36.0	36.0	36.0	36.0
12	34.89074160852511	36.0	36.0	36.0	32.0	36.0
13	34.952605956471935	36.0	36.0	36.0	36.0	36.0
14	34.88645041480093	36.0	36.0	36.0	32.0	36.0
15	34.86140037256876	36.0	36.0	36.0	32.0	36.0
16	34.8793013178752	36.0	36.0	36.0	32.0	36.0
17	34.82845927777199	36.0	36.0	36.0	32.0	36.0
18	34.83474348293936	36.0	36.0	36.0	32.0	36.0
19	34.83110602010946	36.0	36.0	36.0	32.0	36.0
20	34.82624353500642	36.0	36.0	36.0	32.0	36.0
21	34.82238334085413	36.0	36.0	36.0	32.0	36.0
22	34.79361918150578	36.0	36.0	36.0	32.0	36.0
23	34.74401228782672	36.0	36.0	36.0	32.0	36.0
24	34.71162368241406	36.0	36.0	36.0	32.0	36.0
25	34.68139730639731	36.0	36.0	36.0	32.0	36.0
26	34.61472398671712	36.0	36.0	36.0	32.0	36.0
27	34.61817487590683	36.0	36.0	36.0	32.0	36.0
28	34.58335792055723	36.0	36.0	36.0	32.0	36.0
29	34.551374281185275	36.0	36.0	36.0	32.0	36.0
30	34.52603353118817	36.0	36.0	36.0	32.0	36.0
31	34.510964455552084	36.0	36.0	36.0	32.0	36.0
32	34.47250859106529	36.0	36.0	36.0	32.0	36.0
33	34.45134043759473	36.0	36.0	36.0	32.0	36.0
34	34.429220613928514	36.0	36.0	36.0	32.0	36.0
35	34.384565876404366	36.0	36.0	36.0	32.0	36.0
36	34.35365828965485	36.0	36.0	36.0	32.0	36.0
37	34.33767804042718	36.0	36.0	36.0	32.0	36.0
38	34.29120529464172	36.0	36.0	36.0	32.0	36.0
39	34.28453203281382	36.0	36.0	36.0	32.0	36.0
40	34.27228470269707	36.0	36.0	36.0	32.0	36.0
41	34.243255869114975	36.0	36.0	36.0	32.0	36.0
42	34.16967643213348	36.0	36.0	36.0	32.0	36.0
43	34.16736234047231	36.0	36.0	36.0	32.0	36.0
44	34.094328161338474	36.0	36.0	36.0	32.0	36.0
45	34.04276152128386	36.0	36.0	36.0	32.0	36.0
46	34.028408367755446	36.0	36.0	36.0	32.0	36.0
47	33.99438688141437	36.0	36.0	36.0	32.0	36.0
48	33.942037789116824	36.0	36.0	36.0	32.0	36.0
49	33.92527074872436	36.0	36.0	36.0	32.0	36.0
50	33.24844377335786	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	2.0
21	20.0
22	79.0
23	231.0
24	663.0
25	1405.0
26	2875.0
27	5360.0
28	9217.0
29	14261.0
30	20732.0
31	30018.0
32	43797.0
33	70876.0
34	140486.0
35	351391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.76661787423123	18.677859998495727	11.97618042015494	26.579341707118104
2	15.80184347126826	21.08638397021751	36.70729361467025	26.404478943843984
3	17.710781513273506	24.944787139416995	29.26520251947094	28.079228827838566
4	12.053524940122879	16.28975898735349	36.69064644150555	34.96606963101808
5	14.144885278905898	37.20842445069249	33.3612470639962	15.285443206405406
6	33.682616543441284	36.60444652712694	16.62660395478265	13.086332974649126
7	29.561942448540385	30.96370347229454	21.25131613963229	18.223037939532784
8	27.98300878197785	32.97378134147893	19.61684427320166	19.426365603341548
9	27.592800167774318	13.612571502954129	18.461827727598152	40.332800601673405
10	15.370263503868165	26.957693881948998	32.135351808542794	25.536690805640045
11	37.63870086894142	20.839118562486263	22.656114408691728	18.866066159880592
12	24.381125111365662	23.825453851227046	29.236378677959436	22.557042359447856
13	29.35181917583149	19.47160303841993	25.51141721578451	25.665160569964073
14	23.492547890919347	19.70104785114584	25.55050150777753	31.25590275015729
15	25.055974358826017	27.415286351513192	22.395239720677825	25.133499568982977
16	25.70659955742613	25.62575027841657	23.8586366989196	24.809013465237705
17	23.983679868559594	25.7384845013711	25.461227394217083	24.81660823585222
18	24.73550617212528	24.863504552258775	26.378368996912126	24.02262027870382
19	25.66833859789186	24.777268677612323	25.40568919434899	24.14870353014683
20	25.709669907855364	24.28779904340821	25.01818739306319	24.98434365567324
21	26.96046952919805	24.192671271709074	24.47412266999896	24.372736529093917
22	25.747162345100488	24.324719127124624	25.012582873407617	24.915535654367268
23	24.43405668400317	23.97731019999653	25.58749341926377	26.001139696736537
24	24.780160020364125	24.995227158336853	25.19785598167229	25.026756839626735
25	24.69453636637123	24.436801425496956	25.691487515331033	25.17717469280078
26	24.644225526086274	25.375083709513884	25.64223096159993	24.33845980279991
27	25.151435381784943	24.730395843699014	25.103705032225214	25.014463742290825
28	24.52798259747698	24.59798609765199	25.845031921017537	25.02899938385349
29	24.491180265083802	24.730114375965425	25.567251563485314	25.21145379546546
30	24.58739215643735	24.97139840467461	25.842969648319702	24.598239790568343
31	25.13889069689067	24.83572495809052	24.72102532504256	25.304359019976253
32	24.722661752922356	25.009184343514697	24.677101623676226	25.59105227988672
33	24.423153969762318	24.42402178802382	25.411020424102787	25.741803818111077
34	25.09249429413619	24.58366719988892	25.563282296346223	24.76055620962866
35	25.901000581430665	24.431514120665664	25.367733201426674	24.299752096476993
36	24.810775080889766	25.10699723593487	24.924751183514545	25.15747649966082
37	25.551132928547254	25.033338443781695	24.818265706673763	24.59726292099729
38	24.739428898730917	24.82549040594996	25.342004090452814	25.09307660486631
39	25.313826594444482	24.755816223956263	24.62636591768696	25.30399126391229
40	25.661663781221026	24.93328762903149	25.155011346526834	24.25003724322065
41	24.478617495657254	25.27672794963139	25.71367823623086	24.530976318480494
42	25.998767721238874	25.399405844935362	24.909097746318352	23.69272868750741
43	24.98853789869672	24.47249023371116	25.34765963416733	25.19131223342479
44	24.749352046837757	25.159818804526417	24.610938837849723	25.479890310786107
45	24.919512300950235	25.43874112321199	24.711097612125947	24.930648963711835
46	24.65892310384676	25.228770647934013	24.696093362433164	25.416212885786067
47	24.92211432488957	24.93527606385902	25.185059835290236	24.95754977596117
48	25.82535674097987	25.329405060471682	24.277333749880675	24.567904448667772
49	24.900240233377833	25.579864942848758	24.51551971258774	25.004375111185677
50	25.002639575700865	25.68256534938581	24.394741675935315	24.920053398978016
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	42.0
2	20.0
3	70.0
4	120.0
5	139.5
6	159.0
7	141.5
8	124.0
9	171.5
10	219.0
11	316.5
12	414.0
13	799.5
14	1185.0
15	1508.0
16	1831.0
17	1990.0
18	2149.0
19	2181.0
20	2213.0
21	2498.5
22	2784.0
23	3154.5
24	3525.0
25	4050.0
26	4575.0
27	5677.0
28	6779.0
29	7837.0
30	8895.0
31	9835.5
32	10776.0
33	12504.5
34	14233.0
35	16408.5
36	18584.0
37	22280.5
38	25977.0
39	27915.0
40	29853.0
41	33103.5
42	36354.0
43	36829.0
44	37304.0
45	41291.5
46	45279.0
47	48489.0
48	51699.0
49	53010.0
50	54321.0
51	52378.0
52	50435.0
53	49916.5
54	49398.0
55	49502.0
56	49606.0
57	48374.0
58	47142.0
59	42537.5
60	37933.0
61	33456.0
62	28979.0
63	25032.0
64	21085.0
65	17761.0
66	14437.0
67	12500.5
68	10564.0
69	9584.5
70	8605.0
71	6684.5
72	4764.0
73	4068.0
74	3372.0
75	2611.5
76	1851.0
77	1678.0
78	1505.0
79	1217.0
80	929.0
81	750.5
82	572.0
83	486.5
84	401.0
85	288.0
86	175.0
87	135.0
88	95.0
89	73.0
90	51.0
91	42.5
92	34.0
93	24.0
94	14.0
95	13.5
96	13.0
97	11.5
98	10.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007520797898804772
2	4.338921864695061E-4
3	1.4463072882316868E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0015909380170548554
10	4.338921864695061E-4
11	0.0
12	0.0
13	5.785229152926747E-4
14	1.4463072882316868E-4
15	0.004049660407048724
16	8.677843729390122E-4
17	0.0
18	1.4463072882316868E-4
19	0.0
20	4.338921864695061E-4
21	0.0
22	0.0
23	5.785229152926747E-4
24	5.785229152926747E-4
25	0.0011570458305853494
26	0.005351336966457241
27	0.004628183322341398
28	0.003181876034109711
29	0.0017355687458780243
30	0.003037245305286542
31	0.00650838279704259
32	0.003181876034109711
33	0.0039050296782255542
34	0.003181876034109711
35	0.002603353118817036
36	0.0062191213393962535
37	0.003181876034109711
38	0.007231536441158434
39	0.004483552593518229
40	0.00216946093234753
41	0.004772814051164567
42	0.002024830203524362
43	0.001012415101762181
44	0.0011570458305853494
45	8.677843729390122E-4
46	4.338921864695061E-4
47	0.002603353118817036
48	0.002603353118817036
49	7.231536441158435E-4
50	0.0024587223899938675
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	691416.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.55623745488206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.71389845217439	51.74270272393223
2	11.158714312214926	13.960943644122153
3	2.8565893830975515	5.360924512804364
4	1.1308663628813447	2.8297097890457685
5	0.5739887025323217	1.7953286786015785
6	0.33952785952178954	1.2743751241675743
7	0.21068808543264228	0.9225897730867192
8	0.14265714573812072	0.7139275426743675
9	0.11732270321676475	0.6605340193148931
>10	0.6093723168884401	7.211118972129264
>50	0.07202575349475587	3.233898761226709
>100	0.06923743436410733	8.260882949335917
>500	0.005111488442784472	2.033063509558479
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	847	0.12250222731322387	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	781	0.11295659921089474	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	774	0.11194418410913257	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	751	0.10861767734619968	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	739	0.10688210860032167	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	734	0.10615895495620581	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	734	0.10615895495620581	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8926145764633735E-4
2	0.0	0.0	0.0	0.0	2.8926145764633735E-4
3	0.0	0.0	0.0	0.0	2.8926145764633735E-4
4	0.0	0.0	0.0	0.0	2.8926145764633735E-4
5	0.0	0.0	0.0	0.0	2.8926145764633735E-4
6	0.0	0.0	0.0	0.0	4.338921864695061E-4
7	0.0	0.0	0.0	0.0	4.338921864695061E-4
8	0.0	0.0	0.0	0.0	4.338921864695061E-4
9	0.0	0.0	0.0	0.0	4.338921864695061E-4
10	0.0	0.0	0.0	0.0	4.338921864695061E-4
11	0.0	0.0	0.0	0.0	4.338921864695061E-4
12	0.0	0.0	0.0	0.0	7.231536441158434E-4
13	0.0	0.0	0.0	0.0	7.231536441158434E-4
14	0.0	0.0	0.0	0.0	7.231536441158434E-4
15	0.0	0.0	0.0	0.0	7.231536441158434E-4
16	0.0	0.0	0.0	0.0	8.677843729390122E-4
17	0.0	0.0	0.0	0.0	0.0010124151017621807
18	0.0	0.0	0.0	0.0	0.0010124151017621807
19	0.0	0.0	0.0	0.0	0.0010124151017621807
20	0.0	0.0	0.0	0.0	0.0010124151017621807
21	0.0	0.0	0.0	0.0	0.0010124151017621807
22	0.0	0.0	0.0	1.4463072882316868E-4	0.0010124151017621807
23	0.0	0.0	0.0	5.785229152926747E-4	0.0010124151017621807
24	0.0	0.0	0.0	8.677843729390122E-4	0.0010124151017621807
25	0.0	0.0	0.0	0.0011570458305853494	0.0010124151017621807
26	0.0	0.0	0.0	0.0011570458305853494	0.0010124151017621807
27	0.0	0.0	0.0	0.0014463072882316868	0.0010124151017621807
28	0.0	0.0	0.0	0.002747983847640205	0.0010124151017621807
29	0.0	0.0	0.0	0.00549596769528041	0.0010124151017621807
30	0.0	0.0	0.0	0.010413412475268145	0.0010124151017621807
31	0.0	0.0	0.0	0.02097145567935946	0.0010124151017621807
32	0.0	0.0	0.0	0.03239728325638978	0.0010124151017621807
33	0.0	0.0	0.0	0.04382311083342011	0.0010124151017621807
34	0.0	0.0	0.0	0.055248938410450435	0.0010124151017621807
35	0.0	0.0	0.0	0.07057979566570632	0.0010124151017621807
36	0.0	0.0	0.0	0.09270829717565113	0.0010124151017621807
37	0.0	0.0	0.0	0.12510558043204092	0.0010124151017621807
38	0.0	0.0	0.0	0.16835016835016836	0.0011570458305853494
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	100	4.6182322E-8	21.999386	5
GTATCAA	1290	0.0	20.63508	1
ACTAGGC	55	0.00448243	19.99944	3
GTCCTAA	125	2.2844688E-8	19.35946	1
GCGGGGA	160	5.2750693E-11	19.252247	36
CGTCGTA	245	0.0	18.856615	10
TACCGTC	260	0.0	18.614866	7
ATACCGT	285	0.0	18.525799	6
GGTCCTA	240	0.0	18.332819	13
AACGCAG	1455	0.0	18.143824	6
ACCGTCG	255	0.0	18.11714	8
CGCAATA	195	1.8189894E-12	18.053389	36
CCGTCGT	275	0.0	17.599508	9
TAGGGTG	75	0.0012903541	17.599508	5
GTATAGG	100	2.4811181E-5	17.599508	1
CGGCTCG	265	0.0	17.435997	36
AATACGA	210	9.094947E-12	16.76265	39
GTTATAG	105	3.771479E-5	16.761436	1
GAATATA	80	0.0019875623	16.49954	1
CTTATAG	80	0.0019875623	16.49954	3
>>END_MODULE