FastQCFastQC Report
Thu 2 Feb 2017
SRR4064037_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064037_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854142
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27860.32617527296398025No Hit
TATCAACGCAGAGTACTTTTTTTTT21250.24878767230741491No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19560.22900173507449578No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12520.14657984269594518No Hit
GTACATGGGGTGGTATCAACGCAAA11610.1359258764936041No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10680.12503775718791488No Hit
GGTATCAACGCAGAGTACTTTTTTT9760.11426671443389975No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG9340.10934949926358849No Hit
GAATAGGACCGCGGTTCTATTTTGT9140.10700796823010694No Hit
GATTAAGAGGGACGGCCGGGGGCAT8910.10431520754160316No Hit
GAATAACGCCGCCGCATCGCCAGTC8600.10068583443970675No Hit
GTATCTGATCGTCTTCGAACCTCCG8560.10021752823301043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC400.005291545314.2438963
ACTCTAA2050.012.97536910
GTAAACG604.0832473E-412.66865817
CGCGGTC1051.989065E-812.66643210
AACCGCG1051.990702E-812.6656897
CCGACCA2050.012.5119649
CGACCAT2000.012.349771510
GCTTTGA2550.012.2989341
GCGTTAT701.0858114E-412.219071
TGCACCG550.003070962412.0885615
GCACGCC1209.969881E-911.87617215
ACGGTAT1459.640644E-1111.7928869
TAAACGC657.998477E-411.69551818
GAACCGC1302.6102498E-911.6914076
TAGGATT658.041841E-411.68798354
TTTGAAC2450.011.627673
CCGTCGT1800.011.6108969
GTATTAC752.0642031E-411.4044661
ACGCCCC1251.8220817E-811.401793517
CGCATCG1501.7462298E-1011.40045613
Produced by FastQC (version 0.11.5)