##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064037_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	854142
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.176419143421118	32.0	32.0	32.0	32.0	32.0
2	30.774718957737708	32.0	32.0	32.0	32.0	32.0
3	30.807623322585705	32.0	32.0	32.0	32.0	32.0
4	30.829054185369646	32.0	32.0	32.0	32.0	32.0
5	30.747118160680543	32.0	32.0	32.0	32.0	32.0
6	34.380688456954466	36.0	36.0	36.0	32.0	36.0
7	34.302855965401534	36.0	36.0	36.0	32.0	36.0
8	34.24171859011734	36.0	36.0	36.0	32.0	36.0
9	34.38355683247048	36.0	36.0	36.0	32.0	36.0
10	34.10285057988016	36.0	36.0	36.0	32.0	36.0
11	34.39614139100993	36.0	36.0	36.0	32.0	36.0
12	34.20107078214161	36.0	36.0	36.0	32.0	36.0
13	34.293498036626225	36.0	36.0	36.0	32.0	36.0
14	34.18464962500381	36.0	36.0	36.0	32.0	36.0
15	34.12343146689895	36.0	36.0	36.0	32.0	36.0
16	34.12616403361502	36.0	36.0	36.0	32.0	36.0
17	34.05178998339854	36.0	36.0	36.0	32.0	36.0
18	34.03489934928852	36.0	36.0	36.0	32.0	36.0
19	34.059949048284714	36.0	36.0	36.0	32.0	36.0
20	34.02084548002557	36.0	36.0	36.0	32.0	36.0
21	33.99454657422302	36.0	36.0	36.0	32.0	36.0
22	33.9815581015803	36.0	36.0	36.0	32.0	36.0
23	33.93275474101496	36.0	36.0	36.0	32.0	36.0
24	33.92112903943372	36.0	36.0	36.0	32.0	36.0
25	33.516311105179234	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	9.0
5	15.0
6	29.0
7	17.0
8	46.0
9	50.0
10	83.0
11	28.0
12	65.0
13	44.0
14	89.0
15	162.0
16	262.0
17	371.0
18	474.0
19	613.0
20	909.0
21	1330.0
22	2134.0
23	3302.0
24	4855.0
25	7064.0
26	10013.0
27	13209.0
28	18011.0
29	24337.0
30	32005.0
31	43946.0
32	61699.0
33	86268.0
34	178812.0
35	363887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.87449623633938	17.249242524504492	11.26181890157562	25.614442337580506
2	16.347653414386272	20.124178659910175	37.994853013492694	25.533314912210862
3	18.420722962095713	24.77400848821894	28.857368652129374	27.947899897555978
4	11.97474318175964	15.534605774927234	36.55904536439271	35.93160567892041
5	13.92255813408894	37.424536917780955	34.035899117158046	14.617005830972063
6	33.48200015221859	36.26455590227567	17.34472240599039	12.908721539515359
7	29.654095083744846	30.75894388866965	21.430670883867332	18.15629014371817
8	28.106521003324975	32.93470329956046	19.7827230128326	19.17605268428197
9	27.656725858595323	13.657288103827952	18.663613362695028	40.022372674881694
10	15.43633045234419	27.097190625578406	32.35232099638959	25.11415792568782
11	37.15582305512571	20.940835243719157	22.861139062421294	19.042202638733837
12	24.322329560231776	23.93959132413137	29.741232869330098	21.99684624630676
13	29.567077606900888	19.68611867085456	25.725335092218454	25.02146863002609
14	23.230941002663005	19.902477543708784	25.980291653535186	30.886289800093024
15	24.79502171323072	27.59237934132824	22.883062508566386	24.729536436874653
16	25.09636180019073	25.86655325849331	24.63839429022651	24.398690651089446
17	23.36387940588631	25.96423977300152	25.940808881245207	24.731071939866958
18	24.1720476975515	25.031489429511133	27.105749413271376	23.690713459665997
19	25.111045237093737	25.12780015020838	25.92383427399087	23.837320338707013
20	25.46312027652469	24.4146698693538	25.628332063975627	24.49387779014588
21	26.63467194079726	24.383519591692306	24.726705207188502	24.25510326032193
22	25.53377467924307	24.67572792782237	25.388130529029233	24.402366863905325
23	24.022839948114463	24.664378609753495	25.878087734366666	25.434693707765373
24	24.433519123672625	25.23073426044939	25.791737504847852	24.544009111030135
25	24.47540075059083	24.88444111681729	25.92901140180981	24.711146730782076
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	103.0
1	103.0
2	291.5
3	480.0
4	480.0
5	480.0
6	1497.5
7	2515.0
8	2515.0
9	2515.0
10	2413.0
11	2311.0
12	2311.0
13	2311.0
14	2402.0
15	2493.0
16	2493.0
17	2493.0
18	4380.0
19	6267.0
20	6267.0
21	6267.0
22	9996.5
23	13726.0
24	13726.0
25	13726.0
26	20695.5
27	27665.0
28	27665.0
29	27665.0
30	34687.5
31	41710.0
32	41710.0
33	41710.0
34	49913.5
35	58117.0
36	58117.0
37	58117.0
38	65280.0
39	72443.0
40	72443.0
41	72443.0
42	81742.0
43	91041.0
44	91041.0
45	91041.0
46	102094.0
47	113147.0
48	113147.0
49	113147.0
50	112793.5
51	112440.0
52	112440.0
53	112440.0
54	103466.0
55	94492.0
56	94492.0
57	94492.0
58	86341.0
59	78190.0
60	78190.0
61	78190.0
62	68515.5
63	58841.0
64	58841.0
65	58841.0
66	48246.0
67	37651.0
68	37651.0
69	37651.0
70	28424.5
71	19198.0
72	19198.0
73	19198.0
74	14689.5
75	10181.0
76	10181.0
77	10181.0
78	8188.0
79	6195.0
80	6195.0
81	6195.0
82	4279.0
83	2363.0
84	2363.0
85	2363.0
86	1801.5
87	1240.0
88	1240.0
89	1240.0
90	894.0
91	548.0
92	548.0
93	548.0
94	388.0
95	228.0
96	228.0
97	228.0
98	392.5
99	557.0
100	557.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03734741998403076
2	0.00585382758370388
3	0.0019903013784593193
4	0.004214755860266794
5	0.009600277237274364
6	0.012527191029126304
7	0.020956702749659894
8	0.03524004205389736
9	0.04940630480646075
10	0.05853827583703881
11	0.05561136204518687
12	0.06509456273078716
13	0.06685071100589832
14	0.06966054824607618
15	0.06029442411214997
16	0.06884101238435764
17	0.06649948135087608
18	0.07972913169004685
19	0.0773876006565653
20	0.08101697375846172
21	0.07820713651828384
22	0.08101697375846172
23	0.08488049996370627
24	0.07855836617330608
25	0.07972913169004685
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	854142.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.193012449049796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.94814894187873	41.15858981089522
2	14.437807661089149	15.648565806287815
3	4.592537516259743	7.466503283741773
4	1.873915484555962	4.062125007320336
5	0.9401715568410437	2.54753644420646
6	0.5355445714672964	1.7413664177128938
7	0.33560945990172103	1.2731381346930992
8	0.22073577112801832	0.9569869114153042
9	0.1587074876279505	0.7740753167501044
>10	0.7780028896456548	7.738880809159733
>50	0.08430443195841583	3.2202175703672746
>100	0.0853887160204131	9.281907644233653
>500	0.007821867107930362	2.9116570309914316
>1k	0.0013036445179883938	1.2184498122249812
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2786	0.32617527296398025	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2125	0.24878767230741491	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1956	0.22900173507449578	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1252	0.14657984269594518	No Hit
GTACATGGGGTGGTATCAACGCAAA	1161	0.1359258764936041	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1068	0.12503775718791488	No Hit
GGTATCAACGCAGAGTACTTTTTTT	976	0.11426671443389975	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	934	0.10934949926358849	No Hit
GAATAGGACCGCGGTTCTATTTTGT	914	0.10700796823010694	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	891	0.10431520754160316	No Hit
GAATAACGCCGCCGCATCGCCAGTC	860	0.10068583443970675	No Hit
GTATCTGATCGTCTTCGAACCTCCG	856	0.10021752823301043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3415310334815522E-4
2	0.0	0.0	0.0	0.0	2.3415310334815522E-4
3	0.0	0.0	0.0	1.1707655167407761E-4	2.3415310334815522E-4
4	0.0	0.0	0.0	1.1707655167407761E-4	2.3415310334815522E-4
5	0.0	0.0	0.0	2.3415310334815522E-4	2.3415310334815522E-4
6	0.0	0.0	0.0	2.3415310334815522E-4	2.3415310334815522E-4
7	0.0	0.0	0.0	2.3415310334815522E-4	2.3415310334815522E-4
8	0.0	0.0	0.0	2.3415310334815522E-4	2.3415310334815522E-4
9	0.0	0.0	0.0	2.3415310334815522E-4	2.3415310334815522E-4
10	0.0	0.0	0.0	3.5122965502223285E-4	2.3415310334815522E-4
11	0.0	0.0	0.0	3.5122965502223285E-4	2.3415310334815522E-4
12	0.0	0.0	0.0	3.5122965502223285E-4	5.85382758370388E-4
13	0.0	0.0	0.0	3.5122965502223285E-4	9.366124133926209E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAGAC	40	0.0052915453	14.243896	3
ACTCTAA	205	0.0	12.975369	10
GTAAACG	60	4.0832473E-4	12.668658	17
CGCGGTC	105	1.989065E-8	12.666432	10
AACCGCG	105	1.990702E-8	12.665689	7
CCGACCA	205	0.0	12.511964	9
CGACCAT	200	0.0	12.3497715	10
GCTTTGA	255	0.0	12.298934	1
GCGTTAT	70	1.0858114E-4	12.21907	1
TGCACCG	55	0.0030709624	12.088561	5
GCACGCC	120	9.969881E-9	11.876172	15
ACGGTAT	145	9.640644E-11	11.792886	9
TAAACGC	65	7.998477E-4	11.695518	18
GAACCGC	130	2.6102498E-9	11.691407	6
TAGGATT	65	8.041841E-4	11.6879835	4
TTTGAAC	245	0.0	11.62767	3
CCGTCGT	180	0.0	11.610896	9
GTATTAC	75	2.0642031E-4	11.404466	1
ACGCCCC	125	1.8220817E-8	11.4017935	17
CGCATCG	150	1.7462298E-10	11.400456	13
>>END_MODULE