FastQCFastQC Report
Thu 2 Feb 2017
SRR4064037_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064037_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854142
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10070.11789608753579617No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9130.10689089167843285No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8840.1034956716798846No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8790.10291028892151421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGG956.862938E-720.8416352
ATACTAC600.00741133318.3329183
ATTAGAC851.4304582E-418.1172373
ACGCTAT2550.018.11617710
GTCCTAC3250.017.5996021
GTCCTAT3150.017.4599231
GAGTTCG1154.2708434E-617.21700144
TATGGCG2700.017.10972416
ATAGCGT1954.9112714E-1116.9226936
TACGCTA2750.016.7986379
TAAGACT1053.7729835E-516.7615264
GTATTGA1451.2482815E-716.689281
GAATAAG1854.2200554E-1016.6482721
ACCGTCG1854.2200554E-1016.6472978
TGGTCGG3200.016.50059137
TCTATAC1206.4034903E-616.4996263
TATACAG2151.2732926E-1116.3717215
GTGTAAT1351.0847871E-616.2959291
TAGCGTA1906.166374E-1016.2092117
CCGTCGT1906.166374E-1016.2092119
Produced by FastQC (version 0.11.5)