##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064037_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	854142
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.210792818992626	32.0	32.0	32.0	32.0	32.0
2	31.3001327648096	32.0	32.0	32.0	32.0	32.0
3	31.41032521524524	32.0	32.0	32.0	32.0	32.0
4	31.517912712406133	32.0	32.0	32.0	32.0	32.0
5	31.438123871674733	32.0	32.0	32.0	32.0	32.0
6	35.01203312798106	36.0	36.0	36.0	36.0	36.0
7	35.03262103959295	36.0	36.0	36.0	36.0	36.0
8	34.97888173160903	36.0	36.0	36.0	36.0	36.0
9	35.108424594505365	36.0	36.0	36.0	36.0	36.0
10	34.93498622008987	36.0	36.0	36.0	32.0	36.0
11	35.109674972077244	36.0	36.0	36.0	36.0	36.0
12	35.00024351922748	36.0	36.0	36.0	36.0	36.0
13	35.05542052726596	36.0	36.0	36.0	36.0	36.0
14	34.991757810762145	36.0	36.0	36.0	36.0	36.0
15	34.95837577358331	36.0	36.0	36.0	36.0	36.0
16	34.97472434325908	36.0	36.0	36.0	36.0	36.0
17	34.92985826712654	36.0	36.0	36.0	32.0	36.0
18	34.929228395278535	36.0	36.0	36.0	36.0	36.0
19	34.92755185905856	36.0	36.0	36.0	36.0	36.0
20	34.917976167897145	36.0	36.0	36.0	32.0	36.0
21	34.90773196962566	36.0	36.0	36.0	32.0	36.0
22	34.89542254098264	36.0	36.0	36.0	32.0	36.0
23	34.84113063167483	36.0	36.0	36.0	32.0	36.0
24	34.81539135178928	36.0	36.0	36.0	32.0	36.0
25	34.77801349190182	36.0	36.0	36.0	32.0	36.0
26	34.71896710383051	36.0	36.0	36.0	32.0	36.0
27	34.71200573206797	36.0	36.0	36.0	32.0	36.0
28	34.69232750526259	36.0	36.0	36.0	32.0	36.0
29	34.65134134605253	36.0	36.0	36.0	32.0	36.0
30	34.62969740394454	36.0	36.0	36.0	32.0	36.0
31	34.62282969342334	36.0	36.0	36.0	32.0	36.0
32	34.57634210705012	36.0	36.0	36.0	32.0	36.0
33	34.549709533075294	36.0	36.0	36.0	32.0	36.0
34	34.53435260179221	36.0	36.0	36.0	32.0	36.0
35	34.485668659309574	36.0	36.0	36.0	32.0	36.0
36	34.45167665329652	36.0	36.0	36.0	32.0	36.0
37	34.426975842424326	36.0	36.0	36.0	32.0	36.0
38	34.38239894537442	36.0	36.0	36.0	32.0	36.0
39	34.363482886920444	36.0	36.0	36.0	32.0	36.0
40	34.34173591744698	36.0	36.0	36.0	32.0	36.0
41	34.31656445883706	36.0	36.0	36.0	32.0	36.0
42	34.23846152044976	36.0	36.0	36.0	32.0	36.0
43	34.23315326959686	36.0	36.0	36.0	32.0	36.0
44	34.15476466442348	36.0	36.0	36.0	32.0	36.0
45	34.10632658269937	36.0	36.0	36.0	32.0	36.0
46	34.076456842070755	36.0	36.0	36.0	32.0	36.0
47	34.03503515808847	36.0	36.0	36.0	32.0	36.0
48	33.97485898129351	36.0	36.0	36.0	32.0	36.0
49	33.950793896096904	36.0	36.0	36.0	32.0	36.0
50	33.32839504438372	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	8.0
21	30.0
22	92.0
23	285.0
24	855.0
25	1777.0
26	3748.0
27	6741.0
28	11109.0
29	16742.0
30	23926.0
31	33958.0
32	48713.0
33	78405.0
34	158417.0
35	469333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.970976855716756	18.136186784095578	11.829729637960043	26.06310672222762
2	15.641833472069536	20.893203565227676	37.27227067257203	26.192692290130765
3	17.720376378138972	25.40587561070128	29.26472327168465	27.609024739475096
4	11.660707470186455	16.178808675840788	36.861435218031666	35.29904863594109
5	13.62700815555259	37.815609114175395	33.77916084210822	14.778221888163795
6	33.626376971429714	36.652465231068945	16.87781496922632	12.843342828275023
7	29.45192889698539	30.970998933431364	21.40712130666951	18.169950862913733
8	28.03643890594304	33.260511718192056	19.522046685445744	19.181002690419156
9	27.683789783264334	13.413563306670662	18.560098720566472	40.34254818949853
10	15.31736786339396	27.33689639225649	32.29641684277076	25.049318901578786
11	37.29883321508601	21.018402092392133	22.729827124763798	18.952937567758056
12	24.325491926976927	23.932465482865243	29.67133061169058	22.070711978467255
13	29.63604284548534	19.63532867601175	25.707876586831883	25.02075189167103
14	23.30838423254295	19.79736319264195	26.017428076694777	30.87682449812033
15	25.019816296781972	27.764358765711073	22.734239165432417	24.481585772074535
16	25.240681530012164	25.812486755637558	24.511875729537532	24.43495598481274
17	23.43556457825514	26.058547641961173	25.846522006879418	24.65936577290427
18	24.352770795454145	24.99645843016551	27.13861060410401	23.512160170276335
19	25.214191551287723	25.02768860447092	25.964066864760195	23.794052979481165
20	25.649338105390342	24.329646579772145	25.592789932317807	24.42822538251971
21	26.746021153391357	24.411163483355228	24.747875645969874	24.09493971728354
22	25.521430302491037	24.601324605656444	25.408919604608606	24.46832548724391
23	24.144136128045034	24.325371690958242	26.11115078728588	25.41934139371084
24	24.61812421631608	25.061728828680106	25.811606775946306	24.508540179057505
25	24.619201382465818	24.613347445224708	26.017941146856554	24.74951002545292
26	24.277893169167942	25.571070298724504	26.19781336567935	23.95322316642821
27	24.82619090549328	25.123697748970258	25.516509815032933	24.533601530503528
28	24.09592709110217	25.073439276343596	26.258419612530982	24.572214020023253
29	24.08095789482311	24.9987413990725	26.15197108613162	24.768329619972768
30	24.08396088569181	25.360491364130233	26.28800438350006	24.267543366677906
31	24.703949756345438	25.185755867731054	25.34990949304396	24.76038488287955
32	24.29093438018445	25.439606049288326	25.28658251738345	24.982877053143774
33	23.934171719738064	24.897640911320792	25.91192376545631	25.256263603484836
34	24.77151868945509	25.095010291318058	26.11196579395353	24.021505225273323
35	25.321472350977675	24.854674476681765	25.93778135782334	23.886071814517216
36	24.108457970077815	25.647654440674316	25.490409585964812	24.753478003283057
37	24.996311950451346	25.581131236023463	25.321562796361125	24.100994017164066
38	24.2365415643141	25.409859098860288	25.70081749356613	24.652781843259483
39	24.918159466104672	25.089099637044843	25.28275377590446	24.709987120946025
40	25.22584648527139	25.482601976303094	25.53950264599822	23.752048892427293
41	24.112003784038198	25.67678319287589	26.132342989343343	24.078870033742568
42	25.38261244216324	25.984864095311238	25.360718768147162	23.27180469437836
43	24.471567559973305	24.985306686335804	25.81269830119537	24.730427452495523
44	24.236474541346166	25.6346223642771	25.232458759205272	24.89644433517146
45	24.431235453068144	25.84600506713248	25.18486603944121	24.537893440358165
46	24.208876135215476	25.492634051137237	25.239980190438725	25.058509623208565
47	24.426515681033482	25.332476311904284	25.805477209903515	24.435530797158716
48	25.11646367234926	25.93179718754427	24.67109769133234	24.280641448774134
49	24.330200342802552	26.15543660494348	25.02224470107805	24.492118351175925
50	24.534085134980085	26.286402708733842	24.706659204848	24.472852951438078
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	27.0
2	27.0
3	74.0
4	121.0
5	137.5
6	154.0
7	169.5
8	185.0
9	246.5
10	308.0
11	472.0
12	636.0
13	1141.5
14	1647.0
15	2217.5
16	2788.0
17	3156.5
18	3525.0
19	3699.0
20	3873.0
21	4229.5
22	4586.0
23	5209.0
24	5832.0
25	6085.5
26	6339.0
27	7698.0
28	9057.0
29	10720.0
30	12383.0
31	14152.5
32	15922.0
33	18410.5
34	20899.0
35	23885.5
36	26872.0
37	30732.0
38	34592.0
39	36839.0
40	39086.0
41	42157.0
42	45228.0
43	46033.5
44	46839.0
45	51427.0
46	56015.0
47	59087.5
48	62160.0
49	63996.5
50	65833.0
51	63068.0
52	60303.0
53	59059.5
54	57816.0
55	57678.5
56	57541.0
57	56010.0
58	54479.0
59	49507.0
60	44535.0
61	39008.5
62	33482.0
63	28825.0
64	24168.0
65	20745.0
66	17322.0
67	14894.0
68	12466.0
69	11434.5
70	10403.0
71	8030.0
72	5657.0
73	4865.0
74	4073.0
75	3275.0
76	2477.0
77	2142.0
78	1807.0
79	1460.0
80	1113.0
81	905.0
82	697.0
83	578.5
84	460.0
85	330.5
86	201.0
87	139.5
88	78.0
89	66.0
90	54.0
91	38.0
92	22.0
93	21.0
94	20.0
95	19.0
96	18.0
97	13.0
98	8.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007609975858815044
2	3.5122965502223285E-4
3	1.1707655167407762E-4
4	0.0
5	0.0
6	3.5122965502223285E-4
7	1.1707655167407762E-4
8	0.0
9	0.0016390717234370865
10	8.195358617185432E-4
11	0.0
12	1.1707655167407762E-4
13	3.5122965502223285E-4
14	3.5122965502223285E-4
15	0.005502597928681648
16	5.85382758370388E-4
17	0.0
18	1.1707655167407762E-4
19	0.0
20	3.5122965502223285E-4
21	0.0
22	1.1707655167407762E-4
23	5.85382758370388E-4
24	3.5122965502223285E-4
25	0.001873224826785242
26	0.0051513682736594145
27	0.0051513682736594145
28	0.004097679308592716
29	0.0022244544818074744
30	0.0035122965502223285
31	0.007492899307140968
32	0.00292691379185194
33	0.004800138618637182
34	0.0028098372401778628
35	0.00292691379185194
36	0.007024593100444657
37	0.003746449653570484
38	0.007961205513837277
39	0.00491721517031126
40	0.0025756841368297072
41	0.003278143446874173
42	0.0016390717234370865
43	0.0014049186200889314
44	0.0014049186200889314
45	0.001170765516740776
46	0.0010536889650666985
47	0.00245860758515563
48	0.001521995171763009
49	7.024593100444657E-4
50	0.001873224826785242
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	854142.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.94428965292694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.18836746061135	44.72625121974225
2	13.86406795823508	16.066871390796738
3	4.340484972814012	7.545189554967354
4	1.7668640618540652	4.095187319096759
5	0.8699787227404842	2.520514955117902
6	0.5054517018494754	1.7572823890518525
7	0.30867872555756504	1.2520318638382726
8	0.1962614383069273	0.9097783703165317
9	0.15273529529735483	0.7965124371841732
>10	0.6626254879550695	7.028326563465261
>50	0.06929703240934444	2.862188058873445
>100	0.06888767910971438	7.90534626172072
>500	0.006096254767255176	2.415947664463307
>1k	2.0320849224183922E-4	0.11857195136536457
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1007	0.11789608753579617	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	913	0.10689089167843285	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	884	0.1034956716798846	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	879	0.10291028892151421	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.1707655167407761E-4	0.0
4	0.0	0.0	0.0	1.1707655167407761E-4	0.0
5	0.0	0.0	0.0	1.1707655167407761E-4	0.0
6	0.0	0.0	0.0	1.1707655167407761E-4	0.0
7	0.0	0.0	0.0	1.1707655167407761E-4	0.0
8	0.0	0.0	0.0	1.1707655167407761E-4	0.0
9	0.0	0.0	0.0	1.1707655167407761E-4	0.0
10	0.0	0.0	0.0	3.5122965502223285E-4	1.1707655167407761E-4
11	0.0	0.0	0.0	3.5122965502223285E-4	1.1707655167407761E-4
12	0.0	0.0	0.0	3.5122965502223285E-4	3.5122965502223285E-4
13	0.0	0.0	0.0	5.85382758370388E-4	5.85382758370388E-4
14	0.0	0.0	0.0	5.85382758370388E-4	5.85382758370388E-4
15	0.0	0.0	0.0	7.024593100444657E-4	5.85382758370388E-4
16	0.0	0.0	0.0	7.024593100444657E-4	5.85382758370388E-4
17	0.0	0.0	0.0	8.195358617185432E-4	5.85382758370388E-4
18	0.0	0.0	0.0	8.195358617185432E-4	5.85382758370388E-4
19	0.0	0.0	0.0	0.001170765516740776	5.85382758370388E-4
20	0.0	0.0	0.0	0.0012878420684148538	0.0010536889650666985
21	0.0	0.0	0.0	0.0016390717234370865	0.0010536889650666985
22	0.0	0.0	0.0	0.0018732248267852418	0.0014049186200889314
23	0.0	0.0	0.0	0.0025756841368297077	0.0014049186200889314
24	0.0	0.0	0.0	0.0045659855152890266	0.0014049186200889314
25	0.0	0.0	0.0	0.005970904135377958	0.001521995171763009
26	0.0	0.0	0.0	0.006907516548770579	0.001521995171763009
27	0.0	0.0	0.0	0.008195358617185433	0.001521995171763009
28	0.0	0.0	0.0	0.010185659995644752	0.001521995171763009
29	0.0	0.0	0.0	0.0156882579243264	0.0016390717234370865
30	0.0	0.0	0.0	0.027630066195082317	0.0016390717234370865
31	0.0	0.0	0.0	0.05081122342654969	0.0016390717234370865
32	0.0	0.0	0.0	0.07328992134797259	0.0016390717234370865
33	0.0	0.0	0.0	0.09249047582252132	0.0016390717234370865
34	0.0	0.0	0.0	0.11461794408892198	0.0018732248267852418
35	0.0	0.0	0.0	0.1428333930423747	0.0018732248267852418
36	0.0	0.0	0.0	0.18064911923310176	0.0018732248267852418
37	0.0	0.0	0.0	0.2326311081763922	0.0018732248267852418
38	0.0	0.0	0.0	0.30615518262771296	0.0018732248267852418
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTAGG	95	6.862938E-7	20.841635	2
ATACTAC	60	0.007411333	18.332918	3
ATTAGAC	85	1.4304582E-4	18.117237	3
ACGCTAT	255	0.0	18.116177	10
GTCCTAC	325	0.0	17.599602	1
GTCCTAT	315	0.0	17.459923	1
GAGTTCG	115	4.2708434E-6	17.217001	44
TATGGCG	270	0.0	17.109724	16
ATAGCGT	195	4.9112714E-11	16.922693	6
TACGCTA	275	0.0	16.798637	9
TAAGACT	105	3.7729835E-5	16.761526	4
GTATTGA	145	1.2482815E-7	16.68928	1
GAATAAG	185	4.2200554E-10	16.648272	1
ACCGTCG	185	4.2200554E-10	16.647297	8
TGGTCGG	320	0.0	16.500591	37
TCTATAC	120	6.4034903E-6	16.499626	3
TATACAG	215	1.2732926E-11	16.371721	5
GTGTAAT	135	1.0847871E-6	16.295929	1
TAGCGTA	190	6.166374E-10	16.209211	7
CCGTCGT	190	6.166374E-10	16.209211	9
>>END_MODULE