FastQCFastQC Report
Thu 2 Feb 2017
SRR4064036_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064036_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315571
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39100.1688568391986253No Hit
TATCAACGCAGAGTACTTTTTTTTT26590.11483128783354084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6750.015.0543791
TGTTACG500.001499689913.3002877
GTATCAA20300.012.5845911
GGTATAA550.00307668512.086991
TACCGTC951.04022E-612.0002597
GTCTTAA1950.011.6885191
ACACCGT1354.7348294E-911.2587726
CTATCGG600.005891327311.0797411
GTTCGCA909.5104275E-510.5564699
GTATAAT2650.010.3928571
GTCCTAC4400.010.3602771
ACCGTCC1303.8388316E-710.2316548
ATAGCGT750.002649358210.1328956
TAGACAG3000.010.1311435
CGTCTAT750.002655539410.1300491
ACCGGTC856.5902964E-410.0596938
ACCGTCT1701.5570549E-910.0596938
ACCGTCG1054.102665E-59.9532418
GTAGGAC3150.09.9493693
AAGGGCG1451.6229751E-79.8254625
Produced by FastQC (version 0.11.5)