##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064036_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2315571
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276079204654057	32.0	32.0	32.0	32.0	32.0
2	30.841064687716333	32.0	32.0	32.0	32.0	32.0
3	30.855338488865165	32.0	32.0	32.0	32.0	32.0
4	30.891975672523106	32.0	32.0	32.0	32.0	32.0
5	30.779767927651537	32.0	32.0	32.0	32.0	32.0
6	34.44608651602564	36.0	36.0	36.0	32.0	36.0
7	34.40569647832003	36.0	36.0	36.0	32.0	36.0
8	34.34938466581245	36.0	36.0	36.0	32.0	36.0
9	34.519091403373075	36.0	36.0	36.0	32.0	36.0
10	34.183553862092765	36.0	36.0	36.0	32.0	36.0
11	34.49693833615985	36.0	36.0	36.0	32.0	36.0
12	34.301767900876285	36.0	36.0	36.0	32.0	36.0
13	34.3793975654385	36.0	36.0	36.0	32.0	36.0
14	34.27236521790954	36.0	36.0	36.0	32.0	36.0
15	34.206363786729064	36.0	36.0	36.0	32.0	36.0
16	34.21214076355249	36.0	36.0	36.0	32.0	36.0
17	34.15701742680315	36.0	36.0	36.0	32.0	36.0
18	34.14502340891296	36.0	36.0	36.0	32.0	36.0
19	34.15617962049102	36.0	36.0	36.0	32.0	36.0
20	34.14307183843639	36.0	36.0	36.0	32.0	36.0
21	34.14583962227891	36.0	36.0	36.0	32.0	36.0
22	34.108184115278696	36.0	36.0	36.0	32.0	36.0
23	34.043889390565006	36.0	36.0	36.0	32.0	36.0
24	34.04747252405562	36.0	36.0	36.0	32.0	36.0
25	33.62155252419382	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	14.0
5	30.0
6	104.0
7	47.0
8	128.0
9	151.0
10	229.0
11	72.0
12	144.0
13	111.0
14	189.0
15	388.0
16	624.0
17	773.0
18	1093.0
19	1377.0
20	2005.0
21	3130.0
22	4902.0
23	7809.0
24	11481.0
25	17503.0
26	25012.0
27	33852.0
28	46256.0
29	62729.0
30	83408.0
31	113233.0
32	160318.0
33	227579.0
34	485400.0
35	1025476.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.269983907027985	16.777603767267543	11.40350157149492	26.548910754209555
2	16.603661946891187	19.332911526343864	37.53713665034864	26.52628987641631
3	18.89859095685116	22.927753878895793	28.63786161604427	29.535793548208776
4	13.10939176889507	15.279822585764968	35.455595695934115	36.155189949405845
5	14.740886498663706	36.215683462931835	34.21879831870348	14.824631719700987
6	34.75399696621022	35.123372966099645	16.569010079230978	13.55361998845916
7	30.468505671657265	30.2549869979525	20.784917624901727	18.491589705488508
8	28.04762721478602	33.727173874068086	19.115904380948596	19.1092945301973
9	27.369776420085685	14.343119230378697	18.199910559392666	40.087193790142955
10	15.664306171798165	27.2921488338971	32.58956427350398	24.453980720800757
11	36.735171833288824	21.064582560954214	22.604760534386966	19.595485071369993
12	24.293001359504736	23.36900484771905	29.461280043282894	22.87671374949332
13	29.958756079200082	19.890857453507678	24.66126312532465	25.48912334196759
14	23.493098323554467	18.964279648729928	25.52956932207953	32.01305270563608
15	25.065541432512955	27.15772507383809	22.223849865071287	25.552883628577668
16	25.453793507586237	25.8299398485648	23.71260414357347	25.003662500275496
17	24.244828499656666	25.83202719702067	25.26324750645722	24.659896796865446
18	24.50288965377747	25.62119344516227	25.783785092048827	24.092131809011434
19	25.562100768426237	25.110898859894025	25.308580615605365	24.018419756074373
20	25.701535628924972	24.924817716999968	25.073972105406465	24.299674548668587
21	25.750092486628333	24.70546898499815	24.867493456787134	24.676945071586378
22	25.812560994479057	24.556712223617755	25.072112870364528	24.558613911538664
23	24.98091749876169	24.807683829535996	25.178225807148742	25.03317286455357
24	24.823102096843066	24.906991378550273	25.10882700223532	25.161079522371338
25	25.002150230906707	24.77977961753983	25.25659061980676	24.9614795317467
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	63.0
1	63.0
2	242.5
3	422.0
4	422.0
5	422.0
6	1147.5
7	1873.0
8	1873.0
9	1873.0
10	2548.0
11	3223.0
12	3223.0
13	3223.0
14	4789.5
15	6356.0
16	6356.0
17	6356.0
18	10941.0
19	15526.0
20	15526.0
21	15526.0
22	23230.0
23	30934.0
24	30934.0
25	30934.0
26	45772.5
27	60611.0
28	60611.0
29	60611.0
30	79043.0
31	97475.0
32	97475.0
33	97475.0
34	119527.5
35	141580.0
36	141580.0
37	141580.0
38	168667.5
39	195755.0
40	195755.0
41	195755.0
42	223148.5
43	250542.0
44	250542.0
45	250542.0
46	275075.0
47	299608.0
48	299608.0
49	299608.0
50	308378.5
51	317149.0
52	317149.0
53	317149.0
54	302220.5
55	287292.0
56	287292.0
57	287292.0
58	260719.0
59	234146.0
60	234146.0
61	234146.0
62	201635.0
63	169124.0
64	169124.0
65	169124.0
66	136665.0
67	104206.0
68	104206.0
69	104206.0
70	78283.0
71	52360.0
72	52360.0
73	52360.0
74	39294.0
75	26228.0
76	26228.0
77	26228.0
78	19274.0
79	12320.0
80	12320.0
81	12320.0
82	8519.0
83	4718.0
84	4718.0
85	4718.0
86	3313.5
87	1909.0
88	1909.0
89	1909.0
90	1311.0
91	713.0
92	713.0
93	713.0
94	508.0
95	303.0
96	303.0
97	303.0
98	719.0
99	1135.0
100	1135.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038694559570835875
2	0.005873281363430446
3	0.001209204986588621
4	0.0037139867445221933
5	0.009284966861305484
6	0.013603556099121988
7	0.023795426700368934
8	0.036448893167171294
9	0.051218468360503734
10	0.0590351148809516
11	0.0558393588449674
12	0.06447653732060041
13	0.066203973015727
14	0.06771547924896279
15	0.05985564683613675
16	0.06814733817274443
17	0.06451972321297857
18	0.0776914203883189
19	0.07561849755416698
20	0.08036894571576514
21	0.07440929256757836
22	0.07933248429868918
23	0.08347832996699302
24	0.07803690752734423
25	0.08058487517765597
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2315571.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.85486286965075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.87075305206947	24.460114058450223
2	16.72198966305466	13.663491891836307
3	8.060408781499206	9.879206863244363
4	4.664259876329916	7.622307905434915
5	2.84614452313607	5.813942209996582
6	1.868929483526409	4.581291465751118
7	1.3127372690480827	3.754219077758747
8	0.9627086481061732	3.1465063841443652
9	0.69275382170444	2.547212614934524
>10	2.8745472957518317	18.979508832423733
>50	0.08972808438067872	2.460700572437648
>100	0.03344391070208123	2.382778113776618
>500	0.0010637271272456595	0.2742661259454383
>1k	5.318635636226268E-4	0.43445388386558065
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3910	0.1688568391986253	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2659	0.11483128783354084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	675	0.0	15.054379	1
TGTTACG	50	0.0014996899	13.300287	7
GTATCAA	2030	0.0	12.584591	1
GGTATAA	55	0.003076685	12.08699	1
TACCGTC	95	1.04022E-6	12.000259	7
GTCTTAA	195	0.0	11.688519	1
ACACCGT	135	4.7348294E-9	11.258772	6
CTATCGG	60	0.0058913273	11.079741	1
GTTCGCA	90	9.5104275E-5	10.556469	9
GTATAAT	265	0.0	10.392857	1
GTCCTAC	440	0.0	10.360277	1
ACCGTCC	130	3.8388316E-7	10.231654	8
ATAGCGT	75	0.0026493582	10.132895	6
TAGACAG	300	0.0	10.131143	5
CGTCTAT	75	0.0026555394	10.130049	1
ACCGGTC	85	6.5902964E-4	10.059693	8
ACCGTCT	170	1.5570549E-9	10.059693	8
ACCGTCG	105	4.102665E-5	9.953241	8
GTAGGAC	315	0.0	9.949369	3
AAGGGCG	145	1.6229751E-7	9.825462	5
>>END_MODULE