FastQCFastQC Report
Thu 2 Feb 2017
SRR4064036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315571
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21450.030.35891
GGTATCA7750.025.2644541
TATGCGC808.9887966E-519.251210
TAGGACG4350.019.2162694
CGCAATA2200.019.00241536
TCAACGC33350.018.9304434
TACCGTA708.1336056E-418.8546547
ATCAACG34100.018.5140863
CAACGCA34350.018.379345
TATCAAC34850.018.368132
AACGCAG35100.018.049296
TCTAGCG1954.9112714E-1116.92303728
CCGATCG1105.6238903E-516.0003079
GCGCAAT2800.015.71628435
TACGAAT2252.5465852E-1115.6437341
TACCGTC2700.015.4794387
ACGCAGA40700.015.4574197
CGCGCTA1158.213802E-515.30629336
ATACCGT2900.015.1707386
GTCCTAC6050.014.9090541
Produced by FastQC (version 0.11.5)