##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064036_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2315571
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.366242278902266	32.0	32.0	32.0	32.0	32.0
2	31.45560511856471	32.0	32.0	32.0	32.0	32.0
3	31.50661111233471	32.0	32.0	32.0	32.0	32.0
4	31.578219367922642	32.0	32.0	32.0	32.0	32.0
5	31.541993745819067	32.0	32.0	32.0	32.0	32.0
6	35.137263767770456	36.0	36.0	36.0	36.0	36.0
7	35.16064806477539	36.0	36.0	36.0	36.0	36.0
8	35.108707096435396	36.0	36.0	36.0	36.0	36.0
9	35.20880810823767	36.0	36.0	36.0	36.0	36.0
10	35.07705442847574	36.0	36.0	36.0	36.0	36.0
11	35.19934780665331	36.0	36.0	36.0	36.0	36.0
12	35.12525074808762	36.0	36.0	36.0	36.0	36.0
13	35.15023465054624	36.0	36.0	36.0	36.0	36.0
14	35.10177489699085	36.0	36.0	36.0	36.0	36.0
15	35.074229639255286	36.0	36.0	36.0	36.0	36.0
16	35.095899888191724	36.0	36.0	36.0	36.0	36.0
17	35.06815122490306	36.0	36.0	36.0	36.0	36.0
18	35.06140299736005	36.0	36.0	36.0	36.0	36.0
19	35.056780379439886	36.0	36.0	36.0	36.0	36.0
20	35.05113987003637	36.0	36.0	36.0	36.0	36.0
21	35.05336739836524	36.0	36.0	36.0	36.0	36.0
22	35.03497970910847	36.0	36.0	36.0	36.0	36.0
23	34.986946200310854	36.0	36.0	36.0	36.0	36.0
24	34.96586155207506	36.0	36.0	36.0	36.0	36.0
25	34.94047602081733	36.0	36.0	36.0	36.0	36.0
26	34.89101996872478	36.0	36.0	36.0	32.0	36.0
27	34.87813243472129	36.0	36.0	36.0	32.0	36.0
28	34.864993990683075	36.0	36.0	36.0	32.0	36.0
29	34.84074554397166	36.0	36.0	36.0	32.0	36.0
30	34.82991365844537	36.0	36.0	36.0	32.0	36.0
31	34.81680414895505	36.0	36.0	36.0	32.0	36.0
32	34.7840636283664	36.0	36.0	36.0	32.0	36.0
33	34.76703974959092	36.0	36.0	36.0	32.0	36.0
34	34.7630243253176	36.0	36.0	36.0	32.0	36.0
35	34.727711221119975	36.0	36.0	36.0	32.0	36.0
36	34.71137270245654	36.0	36.0	36.0	32.0	36.0
37	34.69650466342859	36.0	36.0	36.0	32.0	36.0
38	34.68070294540742	36.0	36.0	36.0	32.0	36.0
39	34.66338324326915	36.0	36.0	36.0	32.0	36.0
40	34.65335807021249	36.0	36.0	36.0	32.0	36.0
41	34.63636053483137	36.0	36.0	36.0	32.0	36.0
42	34.59914941066372	36.0	36.0	36.0	32.0	36.0
43	34.58023269422531	36.0	36.0	36.0	32.0	36.0
44	34.55582532342994	36.0	36.0	36.0	32.0	36.0
45	34.52078774522569	36.0	36.0	36.0	32.0	36.0
46	34.500802609809845	36.0	36.0	36.0	32.0	36.0
47	34.473210711310514	36.0	36.0	36.0	32.0	36.0
48	34.43632909550171	36.0	36.0	36.0	32.0	36.0
49	34.42000957863093	36.0	36.0	36.0	32.0	36.0
50	33.842004844593404	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	2.0
19	11.0
20	18.0
21	53.0
22	189.0
23	617.0
24	1607.0
25	3662.0
26	7724.0
27	14279.0
28	24023.0
29	37059.0
30	53825.0
31	76627.0
32	109977.0
33	176136.0
34	374503.0
35	1435257.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.360528645777094	17.62243289351502	11.912626600729912	27.104411859977972
2	15.906631253913758	19.934529593401134	37.05866856686316	27.100170585821942
3	18.11313274568647	23.551723706070618	28.848578077987945	29.486565470254966
4	12.76777952392736	15.933910037740151	35.39982146952091	35.898488968811584
5	14.352399472959371	36.62275956988579	33.98820420535583	15.036636751799016
6	34.90138903524152	35.42260114598431	16.320775413678916	13.35523440509526
7	30.43178588070578	30.27804397104988	20.712706034672255	18.577464113572084
8	27.95958318704112	33.93348767971269	18.982315808930064	19.12461332431612
9	27.408034410979727	14.15825250265597	18.05665201205766	40.37706107430664
10	15.48708013627905	27.485734447600414	32.69988939959224	24.327296016528305
11	36.96345307485713	21.03070905621119	22.453641024179348	19.55219684475233
12	24.265308898692112	23.462697009153274	29.38893963244392	22.883054459710696
13	30.002604122805764	19.882844704021792	24.736834604516662	25.377716568655778
14	23.481180618984997	18.893099524306162	25.499176226968363	32.126543629740475
15	25.07427221007925	27.275079044461403	22.131412685723713	25.51923605973563
16	25.550247003844873	25.81882238827821	23.739058219836227	24.891872388040685
17	24.285629764753487	25.85332084397326	25.26003305448203	24.60101633679123
18	24.575698057668788	25.6553467658907	25.654526232872456	24.114428943568058
19	25.651405766789935	25.09003186689751	25.323408630880788	23.935153735431765
20	25.77326933723966	24.912483297762353	25.080563734529647	24.233683630468335
21	25.86359908635926	24.619845385868107	24.89355757176092	24.622997956011712
22	25.834752352231654	24.561630045686435	25.013096144486425	24.590521457595482
23	25.003217372602844	24.855563722547604	25.042732754235054	25.098486150614498
24	24.960441466326014	24.89099810153587	25.063872348584965	25.084688083553154
25	25.106800510638593	24.656148133560666	25.323295035438942	24.9137563203618
26	24.915340540274407	25.007590307382614	25.326879880524462	24.750189271818517
27	25.18730878265271	25.12019376115345	24.875141874418034	24.81735558177581
28	25.180589061956105	24.79202998257811	25.13563074233484	24.89175021313094
29	24.975912444380526	25.084139094998903	24.93674182502264	25.00320663559793
30	25.005052939255894	25.084647529415015	25.03532738727627	24.87497214405282
31	25.087284681948613	24.783191933012354	25.020255574385896	25.109267810653137
32	25.062341016372347	25.104621484005545	24.988447369671217	24.844590129950898
33	24.997462706098446	24.836760227669764	25.173367353945803	24.992409712285987
34	25.138588017426937	24.69540003524083	25.189721770195238	24.976290177136992
35	25.038069498536387	24.92712163496296	25.3519971047315	24.682811761769155
36	25.026107777396756	24.920596941697383	25.18603680915884	24.86725847174702
37	25.30289380513393	24.795314010204283	24.988792907616418	24.91299927704537
38	25.017912725830993	24.912487858432673	25.110596775579786	24.95900264015655
39	25.162224014034294	24.89415760335586	25.02272755737386	24.92089082523599
40	25.344717980787003	24.67139073968826	25.169249532285676	24.81464174723906
41	24.929680202013135	24.840498330161225	25.33870756681191	24.891113901013735
42	25.15746982431183	24.617548605649784	25.263320640736453	24.96166092930193
43	24.96007414246859	24.491760460401927	25.37457752871682	25.173587868412667
44	24.669592109337447	24.724654692822043	25.35733457364502	25.24841862419549
45	24.74233927649862	24.758015931454583	25.32155496870067	25.178089823346124
46	24.898242274294347	24.61282399983762	25.140215128248204	25.34871859761983
47	24.79568339606931	24.755390140633395	25.487664995050796	24.961261468246494
48	24.818777599032618	24.903596376631647	25.183316958357178	25.09430906597856
49	24.753784942456612	24.846591986290985	25.219331677000394	25.18029139425201
50	24.586262279548976	25.03371788056075	25.275260123245708	25.104759716644566
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	19.0
2	33.0
3	133.5
4	234.0
5	269.0
6	304.0
7	295.5
8	287.0
9	397.0
10	507.0
11	735.5
12	964.0
13	1757.5
14	2551.0
15	3299.5
16	4048.0
17	4017.5
18	3987.0
19	4069.0
20	4151.0
21	4884.5
22	5618.0
23	7005.5
24	8393.0
25	10902.5
26	13412.0
27	16589.0
28	19766.0
29	23832.5
30	27899.0
31	33066.5
32	38234.0
33	45315.0
34	52396.0
35	59988.5
36	67581.0
37	75347.0
38	83113.0
39	92011.0
40	100909.0
41	110221.0
42	119533.0
43	127345.5
44	135158.0
45	144075.5
46	152993.0
47	159733.5
48	166474.0
49	171732.5
50	176991.0
51	178653.0
52	180315.0
53	180943.5
54	181572.0
55	176184.0
56	170796.0
57	162102.0
58	153408.0
59	141423.0
60	129438.0
61	114125.5
62	98813.0
63	85508.0
64	72203.0
65	59988.0
66	47773.0
67	40455.0
68	33137.0
69	27701.0
70	22265.0
71	18119.5
72	13974.0
73	11783.5
74	9593.0
75	8043.0
76	6493.0
77	5319.0
78	4145.0
79	3393.5
80	2642.0
81	2206.0
82	1770.0
83	1266.5
84	763.0
85	621.0
86	479.0
87	359.5
88	240.0
89	163.0
90	86.0
91	65.0
92	44.0
93	38.5
94	33.0
95	27.5
96	22.0
97	21.0
98	20.0
99	13.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009544082215574474
2	9.069037399414658E-4
3	3.454871390253203E-4
4	0.0
5	0.0
6	3.023012466471553E-4
7	8.637178475633007E-5
8	0.0
9	0.0013387626637231162
10	0.001209204986588621
11	0.0
12	2.5911535426899026E-4
13	5.182307085379805E-4
14	2.5911535426899026E-4
15	0.004404961022572834
16	8.637178475633008E-4
17	0.0
18	1.2955767713449513E-4
19	8.637178475633007E-5
20	5.614166009161455E-4
21	0.0
22	1.7274356951266014E-4
23	7.341601704288057E-4
24	6.477883856724757E-4
25	0.0016842498027484368
26	0.005095935300623475
27	0.006175582610077601
28	0.004016287991169349
29	0.0025911535426899027
30	0.0037571726369003584
31	0.0068665568881282404
32	0.003498057282631368
33	0.004836819946354484
34	0.0032389419283623782
35	0.002979826574093388
36	0.007298415811909892
37	0.003152570143606048
38	0.007687088843313377
39	0.004318589237816505
40	0.002375224080799077
41	0.0033684996054968736
42	0.002072922834151922
43	0.0012523908789667862
44	0.001036461417075961
45	0.001122833201832291
46	9.500896323196309E-4
47	0.002029736941773757
48	0.001986551049395592
49	6.046024932943105E-4
50	0.0014683203408576114
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2315571.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.203342710737395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.703196404579366	26.657843406149816
2	16.21155030976794	14.007863278105306
3	7.7483340790662565	10.04261797965556
4	4.394948294904969	7.5950582952300145
5	2.730030984909143	5.897323212598083
6	1.7788153710989125	4.611046205802831
7	1.2029222359828484	3.6379183130873476
8	0.8736888578453773	3.019702331843725
9	0.6461992736734188	2.5126171811948073
>10	2.615291733342802	18.17358653984925
>50	0.07066711059864568	2.039440325393454
>100	0.024052650753073248	1.701758890278863
>500	2.0179565151893432E-4	0.05217870644030234
>1k	1.0089782575945428E-4	0.051045334370582014
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	8.637178475633009E-5	0.0
17	0.0	0.0	0.0	8.637178475633009E-5	0.0
18	0.0	0.0	0.0	8.637178475633009E-5	0.0
19	0.0	0.0	0.0	8.637178475633009E-5	0.0
20	0.0	0.0	0.0	1.7274356951266017E-4	0.0
21	0.0	0.0	0.0	1.7274356951266017E-4	0.0
22	0.0	0.0	0.0	2.5911535426899026E-4	0.0
23	0.0	0.0	0.0	3.8867303140348536E-4	0.0
24	0.0	0.0	0.0	4.318589237816504E-4	0.0
25	0.0	0.0	0.0	4.7504481615981545E-4	0.0
26	0.0	0.0	0.0	5.614166009161455E-4	0.0
27	0.0	0.0	0.0	0.001209204986588621	0.0
28	0.0	0.0	0.0	0.0036708008521440286	0.0
29	0.0	0.0	0.0	0.009932755246977959	0.0
30	0.0	0.0	0.0	0.02193843332810784	0.0
31	0.0	0.0	0.0	0.05186625674617621	0.0
32	0.0	0.0	0.0	0.08343514407461486	0.0
33	0.0	0.0	0.0	0.1143994289097592	0.0
34	0.0	0.0	0.0	0.14769575193332443	0.0
35	0.0	0.0	0.0	0.18699491399745463	0.0
36	0.0	0.0	0.0	0.24460489442992678	0.0
37	0.0	0.0	0.0	0.33978660123140253	0.0
38	0.0	0.0	0.0	0.4702511821058391	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2145	0.0	30.3589	1
GGTATCA	775	0.0	25.264454	1
TATGCGC	80	8.9887966E-5	19.2512	10
TAGGACG	435	0.0	19.216269	4
CGCAATA	220	0.0	19.002415	36
TCAACGC	3335	0.0	18.930443	4
TACCGTA	70	8.1336056E-4	18.854654	7
ATCAACG	3410	0.0	18.514086	3
CAACGCA	3435	0.0	18.37934	5
TATCAAC	3485	0.0	18.36813	2
AACGCAG	3510	0.0	18.04929	6
TCTAGCG	195	4.9112714E-11	16.923037	28
CCGATCG	110	5.6238903E-5	16.000307	9
GCGCAAT	280	0.0	15.716284	35
TACGAAT	225	2.5465852E-11	15.64373	41
TACCGTC	270	0.0	15.479438	7
ACGCAGA	4070	0.0	15.457419	7
CGCGCTA	115	8.213802E-5	15.306293	36
ATACCGT	290	0.0	15.170738	6
GTCCTAC	605	0.0	14.909054	1
>>END_MODULE