FastQCFastQC Report
Thu 2 Feb 2017
SRR4064035_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064035_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240417
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25571.0635687160225775No Hit
TATCAACGCAGAGTACTTTTTTTTT20000.831887928058332No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19780.8227371608496903No Hit
GTACATGGGGTGGTATCAACGCAAA15100.6280753856840406No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14500.6031187478422907No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12420.5166024033242241No Hit
GTCCTAAAGTGTGTATTTCTCATTT11840.49247765341053257No Hit
CTGTAGGACGTGGAATATGGCAAGA10550.4388208820507701No Hit
GTCCTACAGTGTGCATTTCTCATTT10120.42093529159751597No Hit
GTCCTACAGTGGACATTTCTAAATT10030.4171917959212535No Hit
GTATCAACGCAGAGTACATGGGGTG9960.4142801881730493No Hit
CTTTAGGACGTGAAATATGGCGAGG8950.37226984780610356No Hit
GGTATCAACGCAGAGTACTTTTTTT8920.37102201591401607No Hit
TATCAACGCAGAGTACATGGGGTGG7150.2973999342808537No Hit
GTACATGGGTGGTATCAACGCAAAA6840.28450567139594957No Hit
CTGTAGGACCTGGAATATGGCGAGA6790.2824259515758037No Hit
GATATACACTGTTCTACAAATCCCG6500.2703635766189579No Hit
GCAGAGTACTTTTTTTTTTTTTTTT5780.24041561120885796No Hit
TTGTAGAACAGTGTATATCAATGAG5620.23376050778439128No Hit
GTATTAGAGGCACTGCCTGCCCAGT4990.20755603805055384No Hit
GGTATCAACGCAGAGTACATGGGGT4940.20547631823040802No Hit
GTACATGGGAGTGGTATCAACGCAA4780.19882121480594134No Hit
CTGAAGGACCTGGAATATGGCGAGA4660.19382988723759134No Hit
ACGCAGAGTACATGGGGTGGTATCA4570.19008639156132887No Hit
ATTTAGAAATGTCCACTGTAGGACG4510.18759072777715385No Hit
GTGTATATCAATGAGTTACAATGAG4480.18634289588506636No Hit
CCATAGGGTCTTCTCGTCTTATTAT4280.17802401660448305No Hit
GTCCTTCAGTGTGCATTTCTCATTT4260.17719212867642473No Hit
ATCAACGCAGAGTACTTTTTTTTTT3990.16596164164763724No Hit
TTTCTAAATTTTCCACCTTTTTCAG3880.1613862580433164No Hit
CTCTAATACTTGTAATGCTAGAGGT3850.1601384261512289No Hit
GAATATGGCAAGAAAACTGAAAATC3730.15514709858287892No Hit
CCATTGGGATGTCCTGATCCAACAT3610.15015577101452893No Hit
GTGTATATCAATGAGTTACAATGAA3460.14391661155409144No Hit
GTTCTACAGTGTGGTTTTTATCATT3200.13310206848933312No Hit
GTTCTACAAATCCCGTTTCCAACGA3080.12811074092098312No Hit
CTTCTACACCATTGGGATGTCCTGA2940.1222875254245748No Hit
CTGTAGGACATGGAATATGGCAAGA2880.1197918616403998No Hit
GATATATTTTGATCAACGGACCAAG2820.1172961978562248No Hit
GTATCAACGCAGAGTACATGGGAGT2810.11688025389219564No Hit
GTATCAACGCAGAGTACATGGGTGG2730.11355270217996231No Hit
GATATACACTGTTCTACAATGCCGG2720.11313675821593315No Hit
GGAATATGGCGAGAAAACTGAAAAT2710.11272081425190397No Hit
GTCAGGATACCGCGGCCGTTAAACT2560.1064816547914665No Hit
CCTCTAGCATTACAAGTATTAGAGG2520.10481787893534983No Hit
ACCTATAACTTCTCTGTTAACCCAA2520.10481787893534983No Hit
GTATCCTGACCGTGCAAAGGTAGCA2470.10273815911520401No Hit
CCTATAACTTCTCTGTTAACCCAAC2430.10107438325908734No Hit
GAAATATGGCGAGGAAAACTGAAAA2420.10065843929505816No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT401.2740935E-519.00645619
CCAACGA654.783942E-1019.00645419
GTTTAGA250.006012996719.0024991
AAATTCC250.006012996719.00249917
TAGGACC2550.016.759934
AACCTTG402.7549258E-416.6202646
AACGGAC350.002161123316.28785715
GGACCAA350.002161123316.28785718
AGGCACT702.474735E-816.2844668
TCTTACA350.00216714516.2810762
TCCAACG1250.015.96209818
CCACCTT1200.015.83541413
CCTGATC755.7656507E-815.20199913
AATCGTA508.6554435E-515.20199913
CACCTTT1200.015.04364414
GACCAAG456.715599E-414.78279919
AGTATTA456.7262165E-414.77972115
ATTGCGA456.7262165E-414.77972117
CAACGGA400.005257336414.25187414
ACGGACC400.005257336414.25187416
Produced by FastQC (version 0.11.5)