##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064035_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240417
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.075360727402803	32.0	32.0	32.0	32.0	32.0
2	30.903280550044297	32.0	32.0	32.0	32.0	32.0
3	30.93664341539908	32.0	32.0	32.0	32.0	32.0
4	30.90813461610452	32.0	32.0	32.0	32.0	32.0
5	30.989231210771283	32.0	32.0	32.0	32.0	32.0
6	34.51514659945012	36.0	36.0	36.0	32.0	36.0
7	34.362790484865876	36.0	36.0	36.0	32.0	36.0
8	34.22978408348827	36.0	36.0	36.0	32.0	36.0
9	34.313783967023966	36.0	36.0	36.0	32.0	36.0
10	34.19264444694011	36.0	36.0	36.0	32.0	36.0
11	34.439024694593144	36.0	36.0	36.0	32.0	36.0
12	34.28610289621782	36.0	36.0	36.0	32.0	36.0
13	34.33569589504902	36.0	36.0	36.0	32.0	36.0
14	34.301101835560715	36.0	36.0	36.0	32.0	36.0
15	34.251937259012465	36.0	36.0	36.0	32.0	36.0
16	34.34011737938665	36.0	36.0	36.0	32.0	36.0
17	34.254657532537216	36.0	36.0	36.0	32.0	36.0
18	34.28311641855609	36.0	36.0	36.0	32.0	36.0
19	34.177653826476494	36.0	36.0	36.0	32.0	36.0
20	34.043249853379756	36.0	36.0	36.0	32.0	36.0
21	34.014520603784256	36.0	36.0	36.0	32.0	36.0
22	33.99401040691798	36.0	36.0	36.0	32.0	36.0
23	34.01037780190253	36.0	36.0	36.0	32.0	36.0
24	33.99518336889654	36.0	36.0	36.0	32.0	36.0
25	33.424242046111544	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	3.0
5	8.0
6	6.0
7	6.0
8	16.0
9	35.0
10	20.0
11	6.0
12	16.0
13	14.0
14	38.0
15	89.0
16	137.0
17	201.0
18	270.0
19	281.0
20	398.0
21	532.0
22	684.0
23	908.0
24	1239.0
25	1629.0
26	2202.0
27	2970.0
28	4133.0
29	5787.0
30	7665.0
31	11375.0
32	16954.0
33	25651.0
34	58256.0
35	98887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.41736228434465	20.731626122489743	12.626397130421033	23.224614462744576
2	12.295763868090912	21.866992778942464	46.29088882233536	19.54635453063126
3	18.57046594624273	29.390135349854834	30.411706473000738	21.627692230901694
4	9.574676067469479	17.549551798007528	41.25371768474033	31.62205444978266
5	9.556394918342388	42.39248899722955	35.86986364052348	12.18125244390459
6	26.60889750480485	41.471491209824364	19.86130409098851	12.058307194382275
7	24.993966930457432	33.79517520866099	22.94147506469947	18.269382796182107
8	24.17024534771464	39.214437897400046	20.415543127267885	16.199773627617432
9	27.57740968869652	14.238804727817547	21.88488430164808	36.29890128183785
10	15.413749760681911	28.478852605029424	35.20514762804559	20.90225000624308
11	32.17352217722636	23.019615514277746	28.371949772962807	16.43491253553309
12	25.037772681298463	26.34805808876476	33.28532838299626	15.328840846940517
13	29.17039546799752	23.201122178424704	28.657173658776173	18.9713086948016
14	19.57659183902831	25.06649572720726	29.764278370469654	25.592634063294774
15	20.493300979359773	35.77805618103797	26.013177446006186	17.715465393596077
16	18.333673269758286	28.623340728684944	33.41602807181122	19.626957929745547
17	16.759485873166508	30.42555316917237	31.661755157085057	21.15320580057606
18	16.978744307254246	28.491203823193935	37.27738970435188	17.252662165199943
19	22.554594423584458	25.764904714726967	30.933787350245183	20.74671351144339
20	22.62501457046276	28.687575974555806	31.65703628461526	17.030373170366175
21	22.795485861054104	25.549177222830455	28.825716105452017	22.829620810663425
22	21.901122323242415	30.434692110433943	29.665384487294766	17.998801079028873
23	20.04554291399716	30.613663706793552	31.72184686091094	17.618946518298344
24	22.0428518501559	28.47354333265341	32.28625069206529	17.197354125125404
25	20.219546005170198	29.984639272675807	33.02098466841227	16.77483005374173
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	112.0
1	112.0
2	285.0
3	458.0
4	458.0
5	458.0
6	1468.5
7	2479.0
8	2479.0
9	2479.0
10	2321.5
11	2164.0
12	2164.0
13	2164.0
14	1759.5
15	1355.0
16	1355.0
17	1355.0
18	2591.0
19	3827.0
20	3827.0
21	3827.0
22	6241.0
23	8655.0
24	8655.0
25	8655.0
26	13769.0
27	18883.0
28	18883.0
29	18883.0
30	24281.0
31	29679.0
32	29679.0
33	29679.0
34	29930.5
35	30182.0
36	30182.0
37	30182.0
38	29400.0
39	28618.0
40	28618.0
41	28618.0
42	27646.5
43	26675.0
44	26675.0
45	26675.0
46	28694.5
47	30714.0
48	30714.0
49	30714.0
50	27612.0
51	24510.0
52	24510.0
53	24510.0
54	18438.0
55	12366.0
56	12366.0
57	12366.0
58	10039.0
59	7712.0
60	7712.0
61	7712.0
62	6462.5
63	5213.0
64	5213.0
65	5213.0
66	4208.5
67	3204.0
68	3204.0
69	3204.0
70	2489.0
71	1774.0
72	1774.0
73	1774.0
74	1369.5
75	965.0
76	965.0
77	965.0
78	716.0
79	467.0
80	467.0
81	467.0
82	322.0
83	177.0
84	177.0
85	177.0
86	117.0
87	57.0
88	57.0
89	57.0
90	42.0
91	27.0
92	27.0
93	27.0
94	20.5
95	14.0
96	14.0
97	14.0
98	72.0
99	130.0
100	130.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0428422282950041
2	0.003743495676262494
3	0.001247831892087498
4	0.004991327568349992
5	0.009566711172670819
6	0.014558038741020811
7	0.031195797302187446
8	0.04367411622306243
9	0.056984323071995746
10	0.06280753856840407
11	0.06155970667631657
12	0.06821481010078323
13	0.07071047388495821
14	0.07320613766913321
15	0.06571914631660823
16	0.07320613766913321
17	0.06863075406481238
18	0.08277284884180403
19	0.0794452971295707
20	0.08526851262597904
21	0.0806931290216582
22	0.08360473676986237
23	0.08485256866194986
24	0.0806931290216582
25	0.0806931290216582
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	240417.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.2624814385006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.7288179577008	24.58811148962012
2	14.973400804463473	10.559985358772467
3	5.512108236903877	5.831118431724878
4	2.683510857897779	3.7850900726654104
5	1.5475895584887411	2.728592404031329
6	0.9495499958715218	2.009009346260872
7	0.6723521709897732	1.6596164164763723
8	0.5072130412729868	1.4308472362603308
9	0.38453825919765977	1.2203795904615728
>10	2.531346945515883	17.269993386490974
>50	0.30196869433926654	7.37635025809323
>100	0.1851917383252533	12.539046739623238
>500	0.01061608691036484	2.766443304757983
>1k	0.0117956521226276	6.235415964761228
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2557	1.0635687160225775	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2000	0.831887928058332	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1978	0.8227371608496903	No Hit
GTACATGGGGTGGTATCAACGCAAA	1510	0.6280753856840406	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1450	0.6031187478422907	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1242	0.5166024033242241	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1184	0.49247765341053257	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1055	0.4388208820507701	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1012	0.42093529159751597	No Hit
GTCCTACAGTGGACATTTCTAAATT	1003	0.4171917959212535	No Hit
GTATCAACGCAGAGTACATGGGGTG	996	0.4142801881730493	No Hit
CTTTAGGACGTGAAATATGGCGAGG	895	0.37226984780610356	No Hit
GGTATCAACGCAGAGTACTTTTTTT	892	0.37102201591401607	No Hit
TATCAACGCAGAGTACATGGGGTGG	715	0.2973999342808537	No Hit
GTACATGGGTGGTATCAACGCAAAA	684	0.28450567139594957	No Hit
CTGTAGGACCTGGAATATGGCGAGA	679	0.2824259515758037	No Hit
GATATACACTGTTCTACAAATCCCG	650	0.2703635766189579	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	578	0.24041561120885796	No Hit
TTGTAGAACAGTGTATATCAATGAG	562	0.23376050778439128	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	499	0.20755603805055384	No Hit
GGTATCAACGCAGAGTACATGGGGT	494	0.20547631823040802	No Hit
GTACATGGGAGTGGTATCAACGCAA	478	0.19882121480594134	No Hit
CTGAAGGACCTGGAATATGGCGAGA	466	0.19382988723759134	No Hit
ACGCAGAGTACATGGGGTGGTATCA	457	0.19008639156132887	No Hit
ATTTAGAAATGTCCACTGTAGGACG	451	0.18759072777715385	No Hit
GTGTATATCAATGAGTTACAATGAG	448	0.18634289588506636	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	428	0.17802401660448305	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	426	0.17719212867642473	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	399	0.16596164164763724	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	388	0.1613862580433164	No Hit
CTCTAATACTTGTAATGCTAGAGGT	385	0.1601384261512289	No Hit
GAATATGGCAAGAAAACTGAAAATC	373	0.15514709858287892	No Hit
CCATTGGGATGTCCTGATCCAACAT	361	0.15015577101452893	No Hit
GTGTATATCAATGAGTTACAATGAA	346	0.14391661155409144	No Hit
GTTCTACAGTGTGGTTTTTATCATT	320	0.13310206848933312	No Hit
GTTCTACAAATCCCGTTTCCAACGA	308	0.12811074092098312	No Hit
CTTCTACACCATTGGGATGTCCTGA	294	0.1222875254245748	No Hit
CTGTAGGACATGGAATATGGCAAGA	288	0.1197918616403998	No Hit
GATATATTTTGATCAACGGACCAAG	282	0.1172961978562248	No Hit
GTATCAACGCAGAGTACATGGGAGT	281	0.11688025389219564	No Hit
GTATCAACGCAGAGTACATGGGTGG	273	0.11355270217996231	No Hit
GATATACACTGTTCTACAATGCCGG	272	0.11313675821593315	No Hit
GGAATATGGCGAGAAAACTGAAAAT	271	0.11272081425190397	No Hit
GTCAGGATACCGCGGCCGTTAAACT	256	0.1064816547914665	No Hit
CCTCTAGCATTACAAGTATTAGAGG	252	0.10481787893534983	No Hit
ACCTATAACTTCTCTGTTAACCCAA	252	0.10481787893534983	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	247	0.10273815911520401	No Hit
CCTATAACTTCTCTGTTAACCCAAC	243	0.10107438325908734	No Hit
GAAATATGGCGAGGAAAACTGAAAA	242	0.10065843929505816	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACGT	40	1.2740935E-5	19.006456	19
CCAACGA	65	4.783942E-10	19.006454	19
GTTTAGA	25	0.0060129967	19.002499	1
AAATTCC	25	0.0060129967	19.002499	17
TAGGACC	255	0.0	16.75993	4
AACCTTG	40	2.7549258E-4	16.620264	6
AACGGAC	35	0.0021611233	16.287857	15
GGACCAA	35	0.0021611233	16.287857	18
AGGCACT	70	2.474735E-8	16.284466	8
TCTTACA	35	0.002167145	16.281076	2
TCCAACG	125	0.0	15.962098	18
CCACCTT	120	0.0	15.835414	13
CCTGATC	75	5.7656507E-8	15.201999	13
AATCGTA	50	8.6554435E-5	15.201999	13
CACCTTT	120	0.0	15.043644	14
GACCAAG	45	6.715599E-4	14.782799	19
AGTATTA	45	6.7262165E-4	14.779721	15
ATTGCGA	45	6.7262165E-4	14.779721	17
CAACGGA	40	0.0052573364	14.251874	14
ACGGACC	40	0.0052573364	14.251874	16
>>END_MODULE