FastQCFastQC Report
Thu 2 Feb 2017
SRR4064035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240417
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10380.4317498346622743No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA7380.3069666454535245No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.303223149777262No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA5650.2350083396764788No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA5490.22835323625201212No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT4750.19757338291385385No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA4350.1809356243526872No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4110.1709529692159872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.16845730543181223No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG4000.1663775856116664No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA3920.16305003389943307No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC3700.15389926669079143No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3650.15181954687064558No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3380.1405890598418581No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT3380.1405890598418581No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.13809339605768312No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA3260.1355977322735081No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA3210.13351801245336228No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG3010.12519913317277895No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC2940.1222875254245748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2730.11355270217996231No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA2680.11147298235981648No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC2550.10606571082743732No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC2540.10564976686340817No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA2530.10523382289937899No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGG207.850809E-443.9923065
CGTATTT250.002348675435.20116430
GGAAGGT250.002348675435.20116429
AGATTTG250.002348675435.20116430
GGACCTA250.002351074235.1938446
GACCTAG250.002351074235.1938447
CACGTAT300.00573505129.33430328
GGTAATA300.00574088629.32820113
ATTGAAC300.00574088629.3282018
CAATAAG703.5715857E-828.28076715
TAGGACC2850.027.784614
AGGACCT4300.027.1115365
GGACCTG4000.026.3953826
TCTCGCA450.001394692224.44525343
TGTAGGA8200.023.878842
CTGTAGG7900.023.6716691
TTAGGAC5250.023.4674433
CTTTAGG5250.023.4674431
TAGGGTC1053.0449883E-923.0435874
AAATTTA4250.022.78196344
Produced by FastQC (version 0.11.5)