##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064035_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240417
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.904703078401276	32.0	32.0	32.0	32.0	32.0
2	31.271461668683994	32.0	32.0	32.0	32.0	32.0
3	31.392597029328208	32.0	32.0	32.0	32.0	32.0
4	31.48601804364916	32.0	32.0	32.0	32.0	32.0
5	31.379827549632513	32.0	32.0	32.0	32.0	32.0
6	34.882429279127514	36.0	36.0	36.0	36.0	36.0
7	34.946850680276356	36.0	36.0	36.0	36.0	36.0
8	34.904050046377755	36.0	36.0	36.0	36.0	36.0
9	35.03539683133888	36.0	36.0	36.0	36.0	36.0
10	34.87497556329211	36.0	36.0	36.0	32.0	36.0
11	35.057408585915304	36.0	36.0	36.0	36.0	36.0
12	34.96113419600111	36.0	36.0	36.0	36.0	36.0
13	35.00713759842274	36.0	36.0	36.0	36.0	36.0
14	34.94527841209232	36.0	36.0	36.0	32.0	36.0
15	34.895202918262854	36.0	36.0	36.0	32.0	36.0
16	34.929726267277275	36.0	36.0	36.0	36.0	36.0
17	34.89316063339947	36.0	36.0	36.0	32.0	36.0
18	34.903434449310986	36.0	36.0	36.0	36.0	36.0
19	34.854136770694254	36.0	36.0	36.0	32.0	36.0
20	34.822063331627966	36.0	36.0	36.0	32.0	36.0
21	34.785426987276274	36.0	36.0	36.0	32.0	36.0
22	34.79382073647038	36.0	36.0	36.0	32.0	36.0
23	34.82048274456465	36.0	36.0	36.0	32.0	36.0
24	34.77810221406972	36.0	36.0	36.0	32.0	36.0
25	34.775510883173816	36.0	36.0	36.0	32.0	36.0
26	34.722802464052045	36.0	36.0	36.0	32.0	36.0
27	34.709147023712966	36.0	36.0	36.0	32.0	36.0
28	34.65873045583299	36.0	36.0	36.0	32.0	36.0
29	34.62436100608526	36.0	36.0	36.0	32.0	36.0
30	34.556117079907	36.0	36.0	36.0	32.0	36.0
31	34.53718331066439	36.0	36.0	36.0	32.0	36.0
32	34.445900248318544	36.0	36.0	36.0	32.0	36.0
33	34.409426122112826	36.0	36.0	36.0	32.0	36.0
34	34.338224834350314	36.0	36.0	36.0	32.0	36.0
35	34.26276012095651	36.0	36.0	36.0	32.0	36.0
36	34.218965380983875	36.0	36.0	36.0	32.0	36.0
37	34.14083446678063	36.0	36.0	36.0	32.0	36.0
38	34.063926427831646	36.0	36.0	36.0	32.0	36.0
39	33.929589005769145	36.0	36.0	36.0	32.0	36.0
40	33.936697488114405	36.0	36.0	36.0	32.0	36.0
41	33.885561337176654	36.0	36.0	36.0	32.0	36.0
42	33.819060216207674	36.0	36.0	36.0	32.0	36.0
43	33.744327564190556	36.0	36.0	36.0	27.0	36.0
44	33.59712915476027	36.0	36.0	36.0	21.0	36.0
45	33.48354317706318	36.0	36.0	36.0	21.0	36.0
46	33.26964399356119	36.0	36.0	36.0	21.0	36.0
47	33.20005240893947	36.0	36.0	36.0	14.0	36.0
48	33.01338091732282	36.0	36.0	36.0	14.0	36.0
49	32.924726620829645	36.0	36.0	36.0	14.0	36.0
50	32.363168993873145	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	3.0
21	7.0
22	29.0
23	100.0
24	222.0
25	626.0
26	1183.0
27	2404.0
28	4072.0
29	5993.0
30	8465.0
31	11616.0
32	16338.0
33	25197.0
34	46793.0
35	117366.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.554557177919214	21.74092100336952	13.267190814925744	23.437331003785516
2	11.905980026370848	22.704717220496054	45.41276199270434	19.976540760428755
3	18.108271114531124	29.916602541438763	30.675706590686936	21.299419753343177
4	9.501823914282268	18.210442689160917	41.452975455146685	30.834757941410135
5	9.394094427598713	42.62219393803267	35.56071326070951	12.422998373659102
6	26.877467067636644	42.01158819883785	19.107217875607798	12.003726857917702
7	24.62679427827483	34.27211886014716	23.059933365776963	18.041153495801048
8	23.87892703095039	39.33166123859794	20.39830793995433	16.391103790497343
9	27.369642619459604	14.026936643151869	22.14328497271396	36.46013576467456
10	15.177379303460237	28.976320310127818	34.669761289759045	21.1765390966529
11	31.946992101224126	23.054526094244583	28.445159868062575	16.553321936468716
12	24.789844312174264	26.663671870125654	33.2102139199807	15.33626989771938
13	29.24543605485469	23.138130831014443	28.789145526314698	18.82728758781617
14	19.650856636593918	24.938752251296705	30.007029452992096	25.403361659117284
15	20.672370977067676	35.480838425477216	26.24634467955891	17.6004459178962
16	18.522893651004924	28.52805137761214	33.08057699986689	19.86847797151604
17	16.8781741723755	30.079403702733167	31.661654541900113	21.38076758299122
18	17.083650490605905	27.952682214652043	37.523552826963154	17.4401144677789
19	22.54873823398512	25.53604778364259	31.747754942454154	20.167459039918143
20	22.410322108345536	28.444446293091975	32.31981232530281	16.825419273259683
21	22.29418052799927	25.297295948289843	30.072748599308703	22.335774924402184
22	21.630333961408716	29.851882354409216	30.820615846633142	17.697167837548925
23	19.760244912899523	30.006405670266044	32.70053075553633	17.532818661298105
24	22.160107148502167	27.935145207849793	32.81797233106225	17.08677531258579
25	20.29241712075205	29.14853791439624	33.51732457052535	17.041720394326358
26	16.879227294282078	30.36746782472692	35.362850558647594	17.39045432234341
27	17.848050716318077	30.864906238040568	33.287575500424296	17.99946754521706
28	16.921931415450658	30.596412705279448	32.43706427513685	20.044591604133043
29	18.61010702683366	28.0391169974252	31.700824019267326	21.649951956473814
30	17.608545543788427	29.430213888172506	32.60817117709209	20.35306939094698
31	20.816919102628635	27.68032081067927	32.12391478882321	19.37884529786889
32	17.39515652687537	31.63149006264403	32.2991106711147	18.674242739365905
33	17.954168243892497	31.137973635716993	33.35551848785987	17.552339632530646
34	18.51186316595122	30.03186250041596	34.3170776346877	17.139196698945128
35	18.343475855528332	31.885926782498014	31.882599092372516	17.887998269601134
36	17.128404798748733	30.65442020665901	33.16694121366412	19.050233780928135
37	18.481620292760844	30.82225115536125	29.911273247615444	20.78485530426246
38	18.693217744350893	33.35510333122566	30.178208925421814	17.77346999900163
39	19.799414299738764	30.147340221967088	30.271718331419823	19.781527146874325
40	21.59745099996672	30.63250806961499	31.10711789957073	16.66292303084756
41	20.7966423885961	28.990179236218278	32.65143983794284	17.561738537242782
42	18.827576453362617	33.9489534458088	30.414961232571297	16.808508868257295
43	16.54111882468918	33.62588545544542	31.690881940660447	18.14211377920495
44	16.968370963179876	32.71508909705007	30.540072874898094	19.77646706487197
45	17.398610706709373	33.064764360883494	30.541158853625056	18.99546607878208
46	19.569657214876067	29.950543439830625	29.076214680570516	21.40358466472279
47	21.613347365093365	31.13927631058996	28.29618105961973	18.951195264696953
48	18.59463911350704	32.1203953279425	27.66006122924362	21.624904329306847
49	20.17710967656063	30.854435644882205	28.974777053107946	19.993677625449223
50	17.971648669783537	33.57735886727784	27.890870672013047	20.560121790925578
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	14.5
2	6.0
3	31.5
4	57.0
5	67.0
6	77.0
7	89.0
8	101.0
9	150.5
10	200.0
11	343.0
12	486.0
13	982.0
14	1478.0
15	2132.5
16	2787.0
17	3372.5
18	3958.0
19	4035.5
20	4113.0
21	4247.5
22	4382.0
23	4496.0
24	4610.0
25	4694.5
26	4779.0
27	6233.5
28	7688.0
29	9322.0
30	10956.0
31	12136.5
32	13317.0
33	15993.5
34	18670.0
35	21917.0
36	25164.0
37	25447.0
38	25730.0
39	23224.5
40	20719.0
41	18959.5
42	17200.0
43	14349.5
44	11499.0
45	10808.0
46	10117.0
47	9438.0
48	8759.0
49	7907.5
50	7056.0
51	6941.5
52	6827.0
53	6199.5
54	5572.0
55	5419.0
56	5266.0
57	5021.5
58	4777.0
59	4116.5
60	3456.0
61	3152.0
62	2848.0
63	2501.0
64	2154.0
65	1888.5
66	1623.0
67	1434.5
68	1246.0
69	1127.5
70	1009.0
71	855.0
72	701.0
73	529.0
74	357.0
75	311.5
76	266.0
77	219.5
78	173.0
79	141.0
80	109.0
81	78.0
82	47.0
83	32.5
84	18.0
85	14.5
86	11.0
87	8.5
88	6.0
89	5.0
90	4.0
91	3.0
92	2.0
93	3.5
94	5.0
95	5.0
96	5.0
97	3.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.011230487028787482
2	0.0
3	8.31887928058332E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	4.15943964029166E-4
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.005823215496408324
16	4.15943964029166E-4
17	0.0
18	0.0
19	0.0
20	4.15943964029166E-4
21	0.0
22	0.0
23	0.00207971982014583
24	0.001247831892087498
25	0.002911607748204162
26	0.0062391594604374895
27	0.008734823244612486
28	0.005407271532379158
29	0.00415943964029166
30	0.004575383604320826
31	0.010814543064758316
32	0.004575383604320826
33	0.006655103424466656
34	0.003743495676262494
35	0.00415943964029166
36	0.008734823244612486
37	0.006655103424466656
38	0.01039859910072915
39	0.008734823244612486
40	0.003743495676262494
41	0.003327551712233328
42	0.003743495676262494
43	0.001663775856116664
44	0.00207971982014583
45	0.002911607748204162
46	0.001663775856116664
47	0.00415943964029166
48	0.003743495676262494
49	4.15943964029166E-4
50	0.00207971982014583
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	240417.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.42348364121446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.34169266047033	31.69246516838953
2	14.949852759295206	13.28249078982231
3	5.320870273768651	7.09114780691358
4	2.5119447590527106	4.463573476456499
5	1.4389181125228179	3.1960877616352295
6	0.8473316330195105	2.2584853762875454
7	0.5685290320929672	1.7679228109716192
8	0.4550210945336109	1.6170897719537085
9	0.32359253359701884	1.2937596860399678
>10	1.9306026683215873	16.140369395271758
>50	0.1861901486002896	5.8022352213327455
>100	0.12047597850606974	9.793649357428697
>500	0.003982676975407264	1.1416045972145004
>1k	9.95669243851816E-4	0.4591187802823135
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1038	0.4317498346622743	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	738	0.3069666454535245	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	729	0.303223149777262	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	565	0.2350083396764788	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	549	0.22835323625201212	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	475	0.19757338291385385	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	435	0.1809356243526872	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	411	0.1709529692159872	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	405	0.16845730543181223	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	400	0.1663775856116664	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	392	0.16305003389943307	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	370	0.15389926669079143	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	365	0.15181954687064558	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	338	0.1405890598418581	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	338	0.1405890598418581	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	332	0.13809339605768312	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	326	0.1355977322735081	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	321	0.13351801245336228	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	301	0.12519913317277895	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	294	0.1222875254245748	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	273	0.11355270217996231	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	268	0.11147298235981648	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC	255	0.10606571082743732	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	254	0.10564976686340817	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	253	0.10523382289937899	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.001663775856116664	0.0
22	0.0	0.0	0.0	0.001663775856116664	0.0
23	0.0	0.0	0.0	0.002911607748204162	0.0
24	0.0	0.0	0.0	0.004575383604320826	0.0
25	0.0	0.0	0.0	0.005407271532379158	0.0
26	0.0	0.0	0.0	0.007071047388495822	0.0
27	0.0	0.0	0.0	0.00831887928058332	0.0
28	0.0	0.0	0.0	0.010814543064758316	0.0
29	0.0	0.0	0.0	0.01746964648922497	0.0
30	0.0	0.0	0.0	0.03493929297844994	0.0
31	0.0	0.0	0.0	0.060727818748258236	0.0
32	0.0	0.0	0.0	0.09441927983462067	0.0
33	0.0	0.0	0.0	0.11771214182025398	0.0
34	0.0	0.0	0.0	0.15223549083467475	0.0
35	0.0	0.0	0.0	0.20048499066205802	0.0
36	0.0	0.0	0.0	0.2657881930146371	0.0
37	0.0	0.0	0.0	0.33941027464779944	0.0
38	0.0	0.0	0.0	0.43632521826659515	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACGG	20	7.850809E-4	43.992306	5
CGTATTT	25	0.0023486754	35.201164	30
GGAAGGT	25	0.0023486754	35.201164	29
AGATTTG	25	0.0023486754	35.201164	30
GGACCTA	25	0.0023510742	35.193844	6
GACCTAG	25	0.0023510742	35.193844	7
CACGTAT	30	0.005735051	29.334303	28
GGTAATA	30	0.005740886	29.328201	13
ATTGAAC	30	0.005740886	29.328201	8
CAATAAG	70	3.5715857E-8	28.280767	15
TAGGACC	285	0.0	27.78461	4
AGGACCT	430	0.0	27.111536	5
GGACCTG	400	0.0	26.395382	6
TCTCGCA	45	0.0013946922	24.445253	43
TGTAGGA	820	0.0	23.87884	2
CTGTAGG	790	0.0	23.671669	1
TTAGGAC	525	0.0	23.467443	3
CTTTAGG	525	0.0	23.467443	1
TAGGGTC	105	3.0449883E-9	23.043587	4
AAATTTA	425	0.0	22.781963	44
>>END_MODULE