FastQCFastQC Report
Thu 2 Feb 2017
SRR4064034_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064034_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953149
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25920.27194069342778515No Hit
TATCAACGCAGAGTACTTTTTTTTT20080.21067010509374715No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18310.19210008088976646No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11290.1184494764197413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG853.6379788E-1217.885327
CGCGGTC901.3460522E-1015.83512810
ACGAGAC551.1267422E-515.54640119
GTATTAG1152.5465852E-1114.0369581
TCGCGTA551.9570132E-413.8197489
CGTCGTA1051.366061E-913.57296810
GAACCGC1205.0931703E-1113.4605676
TGCCCGG852.6915768E-713.41328510
TAGAACC1351.8189894E-1213.3697794
TAGACAG1001.00444595E-813.300815
CCGTCGT1102.73576E-912.9560149
AACGAAC604.085525E-412.66810314
AGAACCG1501.8189894E-1212.6674375
ACGGTAT1155.302354E-912.392719
AACGAGA701.08942506E-412.21502918
CGTCAAT1405.2750693E-1112.21502919
ACTCTAA1251.4042598E-912.16137910
ACGAACG658.0107874E-411.69363315
ATAACGA658.0143195E-411.69301912
TTAGAGT1302.6102498E-911.691794
Produced by FastQC (version 0.11.5)