##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064034_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	953149
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.135819268550875	32.0	32.0	32.0	32.0	32.0
2	30.639010270167624	32.0	32.0	32.0	32.0	32.0
3	30.6710713644981	32.0	32.0	32.0	32.0	32.0
4	30.71510330493973	32.0	32.0	32.0	32.0	32.0
5	30.577491032356956	32.0	32.0	32.0	32.0	32.0
6	34.22727926064026	36.0	36.0	36.0	32.0	36.0
7	34.148987199273144	36.0	36.0	36.0	32.0	36.0
8	34.084361416735476	36.0	36.0	36.0	32.0	36.0
9	34.26931885780712	36.0	36.0	36.0	32.0	36.0
10	33.883291069916666	36.0	36.0	36.0	32.0	36.0
11	34.27426561849197	36.0	36.0	36.0	32.0	36.0
12	34.02519648029846	36.0	36.0	36.0	32.0	36.0
13	34.13917446275451	36.0	36.0	36.0	32.0	36.0
14	34.01877670752422	36.0	36.0	36.0	32.0	36.0
15	33.94579651240257	36.0	36.0	36.0	32.0	36.0
16	33.94015626098333	36.0	36.0	36.0	32.0	36.0
17	33.86255454288889	36.0	36.0	36.0	32.0	36.0
18	33.856406500977286	36.0	36.0	36.0	32.0	36.0
19	33.8797722077031	36.0	36.0	36.0	32.0	36.0
20	33.85264213674882	36.0	36.0	36.0	32.0	36.0
21	33.8317408925572	36.0	36.0	36.0	32.0	36.0
22	33.80756209155127	36.0	36.0	36.0	32.0	36.0
23	33.75784373691836	36.0	36.0	36.0	32.0	36.0
24	33.74068272641528	36.0	36.0	36.0	32.0	36.0
25	33.31591283209656	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	1.0
4	8.0
5	12.0
6	44.0
7	20.0
8	61.0
9	51.0
10	82.0
11	31.0
12	54.0
13	49.0
14	78.0
15	150.0
16	294.0
17	369.0
18	506.0
19	741.0
20	1075.0
21	1732.0
22	2686.0
23	4177.0
24	6348.0
25	9102.0
26	12936.0
27	16907.0
28	22885.0
29	30240.0
30	39279.0
31	52701.0
32	72659.0
33	100257.0
34	201670.0
35	375942.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.058330805714064	16.953275952002873	10.962241041470099	26.02615220081297
2	16.781048648977592	19.864168554380317	37.12030200620506	26.234480790437033
3	18.755350757919125	24.007696698057778	28.638515385002773	28.598437159020328
4	12.558085855327006	15.201103749285238	35.82222502006579	36.418585375321975
5	14.629926332566336	36.69878403092333	33.623659197097375	15.047630439412968
6	34.11338565161231	35.5126320417455	16.96503615375656	13.408946152885637
7	30.211967968165453	30.41513148436344	21.141913284018607	18.2309872634525
8	28.41521279844837	32.69951920806295	19.566689021760006	19.318578971728677
9	27.780815993634793	13.753950701128081	18.373554995964014	40.091678309273114
10	15.735804016539806	26.736201870438197	32.022305012224294	25.505689100797703
11	37.2811396119063	20.879903002818587	22.668157315991415	19.1708000692837
12	24.714887577045708	23.52184050921819	29.16876198794985	22.59450992578626
13	29.851698963383523	19.37320082895723	25.213590867192572	25.561509340466674
14	23.58201559242174	19.368639201103605	25.398366848151316	31.65097835832334
15	25.254708632221206	27.1359569328514	22.349586744758067	25.25974769016932
16	25.633630695222088	25.45368073265042	23.94583863086845	24.96684994125904
17	24.040460243139393	25.596615365234005	25.39137464043505	24.971549751191553
18	24.684374986875206	24.724589351488248	26.32014414172256	24.270891519913985
19	25.514735991096458	24.710372415820586	25.512111126277	24.262780466805957
20	25.862860082844648	24.079337662814932	25.038928584553517	25.0188736697869
21	26.662305946188848	23.963978908951937	24.602757963214557	24.770957181644658
22	25.87396067058862	24.14499111208641	24.972674784259414	25.008373433065557
23	24.61466251271858	24.054782552998013	25.425291411638824	25.905263522644585
24	24.783678303571712	24.778743407451273	25.42269485227336	25.014883436703656
25	24.998687529005608	24.325862398440997	25.422878154392787	25.25257191816061
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	188.0
1	188.0
2	337.0
3	486.0
4	486.0
5	486.0
6	1441.0
7	2396.0
8	2396.0
9	2396.0
10	2319.5
11	2243.0
12	2243.0
13	2243.0
14	2377.0
15	2511.0
16	2511.0
17	2511.0
18	4313.0
19	6115.0
20	6115.0
21	6115.0
22	9632.0
23	13149.0
24	13149.0
25	13149.0
26	19668.5
27	26188.0
28	26188.0
29	26188.0
30	32674.0
31	39160.0
32	39160.0
33	39160.0
34	48353.5
35	57547.0
36	57547.0
37	57547.0
38	67187.5
39	76828.0
40	76828.0
41	76828.0
42	87939.0
43	99050.0
44	99050.0
45	99050.0
46	111335.5
47	123621.0
48	123621.0
49	123621.0
50	127634.5
51	131648.0
52	131648.0
53	131648.0
54	122969.0
55	114290.0
56	114290.0
57	114290.0
58	104764.0
59	95238.0
60	95238.0
61	95238.0
62	82980.0
63	70722.0
64	70722.0
65	70722.0
66	57920.5
67	45119.0
68	45119.0
69	45119.0
70	33830.0
71	22541.0
72	22541.0
73	22541.0
74	17266.0
75	11991.0
76	11991.0
77	11991.0
78	9446.5
79	6902.0
80	6902.0
81	6902.0
82	4720.5
83	2539.0
84	2539.0
85	2539.0
86	1946.5
87	1354.0
88	1354.0
89	1354.0
90	970.0
91	586.0
92	586.0
93	586.0
94	402.0
95	218.0
96	218.0
97	218.0
98	368.5
99	519.0
100	519.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035881063716166096
2	0.005875261895044741
3	0.0013639000827782436
4	0.003567123293420021
5	0.008603062060601229
6	0.014268493173680084
7	0.02329121679821308
8	0.03630072528009787
9	0.049310233761982646
10	0.05644448034882269
11	0.05707397269472034
12	0.06609669631925333
13	0.06578195014630452
14	0.06662127327416804
15	0.061900080679935666
16	0.06945398883070748
17	0.06389347310861157
18	0.07900128941015518
19	0.07543416611673516
20	0.08015535871096754
21	0.07417518142493987
22	0.0767980661995134
23	0.08414214356831932
24	0.07847671245524047
25	0.07837179706425752
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	953149.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.20352119553876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.63882676104225	42.85936618699958
2	13.017620958973817	14.372370290484012
3	3.9765278074830586	6.585550113151209
4	1.7236459467607934	3.806053022224557
5	0.9142473974522114	2.5234837791609652
6	0.5765093748235455	1.9095208495499036
7	0.395736363755102	1.5292228521080147
8	0.28116157372621203	1.2416887115652784
9	0.2193469193725523	1.0897850081482938
>10	1.1046014481172968	10.780073637571522
>50	0.07407804526284249	2.8414202599659473
>100	0.07158851658959808	7.664020023155482
>500	0.005345275810633197	2.000734540342818
>1k	7.636108300904566E-4	0.7967107255723839
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2592	0.27194069342778515	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2008	0.21067010509374715	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1831	0.19210008088976646	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1129	0.1184494764197413	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.049153909829418E-4
2	0.0	0.0	0.0	0.0	1.049153909829418E-4
3	0.0	0.0	0.0	0.0	1.049153909829418E-4
4	0.0	0.0	0.0	0.0	1.049153909829418E-4
5	0.0	0.0	0.0	0.0	2.098307819658836E-4
6	0.0	0.0	0.0	0.0	2.098307819658836E-4
7	0.0	0.0	0.0	0.0	2.098307819658836E-4
8	0.0	0.0	0.0	0.0	2.098307819658836E-4
9	0.0	0.0	0.0	0.0	2.098307819658836E-4
10	0.0	0.0	0.0	0.0	2.098307819658836E-4
11	0.0	0.0	0.0	1.049153909829418E-4	2.098307819658836E-4
12	0.0	0.0	0.0	2.098307819658836E-4	2.098307819658836E-4
13	0.0	0.0	0.0	2.098307819658836E-4	3.147461729488254E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	85	3.6379788E-12	17.88532	7
CGCGGTC	90	1.3460522E-10	15.835128	10
ACGAGAC	55	1.1267422E-5	15.546401	19
GTATTAG	115	2.5465852E-11	14.036958	1
TCGCGTA	55	1.9570132E-4	13.819748	9
CGTCGTA	105	1.366061E-9	13.572968	10
GAACCGC	120	5.0931703E-11	13.460567	6
TGCCCGG	85	2.6915768E-7	13.413285	10
TAGAACC	135	1.8189894E-12	13.369779	4
TAGACAG	100	1.00444595E-8	13.30081	5
CCGTCGT	110	2.73576E-9	12.956014	9
AACGAAC	60	4.085525E-4	12.668103	14
AGAACCG	150	1.8189894E-12	12.667437	5
ACGGTAT	115	5.302354E-9	12.39271	9
AACGAGA	70	1.08942506E-4	12.215029	18
CGTCAAT	140	5.2750693E-11	12.215029	19
ACTCTAA	125	1.4042598E-9	12.161379	10
ACGAACG	65	8.0107874E-4	11.693633	15
ATAACGA	65	8.0143195E-4	11.693019	12
TTAGAGT	130	2.6102498E-9	11.69179	4
>>END_MODULE