FastQCFastQC Report
Thu 2 Feb 2017
SRR4064034_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064034_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953149
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG2600.020.3053938
CTATACT1201.4322723E-820.1643834
ATACCGT2750.019.1978246
TACCGTC2750.019.1978247
TCTATAC1051.79382E-618.8550073
GTCTAGT708.1306574E-418.8540171
CGTCGTA2700.018.74058310
TATACTC1303.5895937E-818.6132775
ACCGCCT951.5980906E-518.5242188
CAATACT951.5980906E-518.5242184
TACGCTC600.007402369318.33702934
GATTTCG1203.1639865E-718.33125741
GTGTAAG1801.4551915E-1118.3302961
GTATCAA18950.018.223891
GTATAAT1456.315531E-918.203881
GTATAAA1456.315531E-918.203881
ACGCTAT2800.018.07127610
ATTTCGT1102.796789E-617.99890542
ATGGGTG3700.017.8358175
TAATACC1002.4845367E-517.5980074
Produced by FastQC (version 0.11.5)