##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064034_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	953149
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.289819325205187	32.0	32.0	32.0	32.0	32.0
2	31.372279675056053	32.0	32.0	32.0	32.0	32.0
3	31.44911446164241	32.0	32.0	32.0	32.0	32.0
4	31.538506571375514	32.0	32.0	32.0	32.0	32.0
5	31.485961796109528	32.0	32.0	32.0	32.0	32.0
6	35.054573838927595	36.0	36.0	36.0	36.0	36.0
7	35.07692081720696	36.0	36.0	36.0	36.0	36.0
8	35.021489819535034	36.0	36.0	36.0	36.0	36.0
9	35.142638768964765	36.0	36.0	36.0	36.0	36.0
10	34.98799663011764	36.0	36.0	36.0	36.0	36.0
11	35.13586752963073	36.0	36.0	36.0	36.0	36.0
12	35.0360122079549	36.0	36.0	36.0	36.0	36.0
13	35.08505385831596	36.0	36.0	36.0	36.0	36.0
14	35.022676412607055	36.0	36.0	36.0	36.0	36.0
15	34.99015578886407	36.0	36.0	36.0	36.0	36.0
16	35.01206946657868	36.0	36.0	36.0	36.0	36.0
17	34.966951651840375	36.0	36.0	36.0	36.0	36.0
18	34.966037838784914	36.0	36.0	36.0	36.0	36.0
19	34.96336564377658	36.0	36.0	36.0	36.0	36.0
20	34.95181026261371	36.0	36.0	36.0	36.0	36.0
21	34.95082300878457	36.0	36.0	36.0	36.0	36.0
22	34.93283526500054	36.0	36.0	36.0	36.0	36.0
23	34.887094252839795	36.0	36.0	36.0	32.0	36.0
24	34.85446766455192	36.0	36.0	36.0	32.0	36.0
25	34.828741361529	36.0	36.0	36.0	32.0	36.0
26	34.76695983524087	36.0	36.0	36.0	32.0	36.0
27	34.76076982717288	36.0	36.0	36.0	32.0	36.0
28	34.73318966919128	36.0	36.0	36.0	32.0	36.0
29	34.70874123563053	36.0	36.0	36.0	32.0	36.0
30	34.68462853132092	36.0	36.0	36.0	32.0	36.0
31	34.679973435423	36.0	36.0	36.0	32.0	36.0
32	34.63164101310498	36.0	36.0	36.0	32.0	36.0
33	34.60999067302174	36.0	36.0	36.0	32.0	36.0
34	34.58976088733241	36.0	36.0	36.0	32.0	36.0
35	34.552014427964565	36.0	36.0	36.0	32.0	36.0
36	34.51693806529724	36.0	36.0	36.0	32.0	36.0
37	34.50672350283114	36.0	36.0	36.0	32.0	36.0
38	34.47030002654359	36.0	36.0	36.0	32.0	36.0
39	34.46116294514289	36.0	36.0	36.0	32.0	36.0
40	34.441518587335246	36.0	36.0	36.0	32.0	36.0
41	34.42312587014202	36.0	36.0	36.0	32.0	36.0
42	34.358409860368106	36.0	36.0	36.0	32.0	36.0
43	34.34415605534917	36.0	36.0	36.0	32.0	36.0
44	34.28038323494018	36.0	36.0	36.0	32.0	36.0
45	34.24199784084125	36.0	36.0	36.0	32.0	36.0
46	34.213189123631246	36.0	36.0	36.0	32.0	36.0
47	34.17411548456747	36.0	36.0	36.0	32.0	36.0
48	34.113753463519345	36.0	36.0	36.0	32.0	36.0
49	34.1053056762374	36.0	36.0	36.0	32.0	36.0
50	33.50639511765736	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	4.0
21	27.0
22	110.0
23	288.0
24	786.0
25	1830.0
26	3845.0
27	7118.0
28	11827.0
29	17857.0
30	25455.0
31	36167.0
32	51516.0
33	81745.0
34	167778.0
35	546793.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.913189915945225	17.821584796064084	11.66610182473672	26.59912346325397
2	15.93227487470453	20.501454654948294	36.795793303656204	26.77047716669097
3	17.88846397977223	24.61587691274675	28.956139936735752	28.53951917074527
4	12.152664483727097	15.835614368792287	36.01661440131606	35.99510674616456
5	14.075239023489509	37.117071937336135	33.53137861971213	15.276310419462224
6	34.17552591572968	35.824799322664816	16.679064194714982	13.320610566890522
7	29.907349023812696	30.64868273204448	21.125177963105376	18.318790281037447
8	28.13075395347422	32.86705436400815	19.48625031343473	19.515941369082903
9	27.72450911990432	13.516028684289214	18.309368557444643	40.45009363836183
10	15.673828586056867	26.79870701458228	32.15110429389924	25.376360105461615
11	37.512917707514774	20.746074328357896	22.559117199934114	19.181890764193216
12	24.569846445672656	23.50883598349889	29.259779173853378	22.661538396975075
13	29.783988341728367	19.35344434151014	25.28865028647328	25.57391703028821
14	23.59772417056341	19.270269958358995	25.421682070026975	31.710323801050627
15	25.20671865803105	27.29357774100914	22.27850598096541	25.221197619994395
16	25.633509347495604	25.445499783348353	23.976045567139455	24.944945302016592
17	24.063498991238514	25.662724295991495	25.35815491596802	24.915621796801968
18	24.78746720075707	24.718432728634724	26.36812579801437	24.12597427259384
19	25.617637554713436	24.60877009656423	25.45187106304582	24.321721285676517
20	25.961267092136225	23.97751439185935	25.113965060856557	24.947253455147862
21	26.688796819804665	23.97043903943665	24.638854995388968	24.701909145369715
22	25.9066797601212	24.076848506213096	25.04700214447284	24.969469589192865
23	24.580257107275614	23.955062356855162	25.603818104943333	25.860862430925895
24	24.82570857529547	24.67242654580363	25.467059051875633	25.03480582702527
25	24.990609885325192	24.177709231689278	25.622003294408945	25.209677588576586
26	24.6014350959546	24.89814889618899	25.82238930151599	24.67802670634042
27	25.137160142401026	24.63594461342821	25.1651744916235	25.061720752547263
28	24.67469651981408	24.534418902330266	25.71591946365058	25.074965114205078
29	24.712864971257645	24.56576958063125	25.479502645513747	25.24186280259736
30	24.57607735421069	24.882753729323216	25.704054675057336	24.83711424140876
31	25.127690738696696	24.537291571362022	24.94470581816503	25.390311871776255
32	24.804746516703037	24.778936545240892	24.824261373174416	25.59205556488165
33	24.532269030292348	24.489356969590144	25.361657391786064	25.616716608331448
34	25.211778604537948	24.523826011050048	25.443963916325156	24.82043146808685
35	25.678694221171167	24.405713044673423	25.396986938644467	24.51860579551094
36	24.661021128275294	25.041995293169638	25.121631589273967	25.175351989281104
37	25.52444104038359	24.83984010240161	24.91380847960886	24.721910377605944
38	24.910710163722516	24.67505214694316	25.296303757517762	25.11793393181656
39	25.365567559570962	24.583598693535983	24.83341324741084	25.21742049948222
40	25.555358542391694	24.744235528887668	25.199161073649467	24.50124485507117
41	24.64723638336112	25.02232149882963	25.507886230127045	24.822555887682203
42	25.62299347420106	25.261241790293138	24.956879367144385	24.158885368361418
43	24.98748857709712	24.522703642424055	25.240339974966663	25.24946780551216
44	24.703793893217867	24.959790670176478	24.92170590376829	25.41470953283736
45	24.83315619895146	25.116955904555173	24.888552222817918	25.16133567367545
46	24.614432297458926	24.90935224625973	24.99045260927041	25.485762847010935
47	24.95339055514813	24.907436842497322	25.220509624574955	24.918662977779597
48	25.402830673675155	25.260142897611026	24.419753863586287	24.917272565127526
49	24.79045773535932	25.32374266772561	24.652284165434786	25.23351543148028
50	24.778231834944684	25.598367492537783	24.59168952981477	25.03171114270277
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	95.0
1	67.0
2	39.0
3	121.5
4	204.0
5	227.5
6	251.0
7	221.5
8	192.0
9	293.5
10	395.0
11	545.0
12	695.0
13	1292.5
14	1890.0
15	2419.0
16	2948.0
17	3116.0
18	3284.0
19	3248.5
20	3213.0
21	3587.0
22	3961.0
23	4394.5
24	4828.0
25	5322.0
26	5816.0
27	7022.0
28	8228.0
29	9622.0
30	11016.0
31	12682.0
32	14348.0
33	16703.5
34	19059.0
35	22129.5
36	25200.0
37	29432.5
38	33665.0
39	36644.5
40	39624.0
41	43200.5
42	46777.0
43	48763.5
44	50750.0
45	55984.5
46	61219.0
47	65049.5
48	68880.0
49	71702.0
50	74524.0
51	73439.5
52	72355.0
53	72171.0
54	71987.0
55	71371.5
56	70756.0
57	68217.0
58	65678.0
59	59854.0
60	54030.0
61	47882.0
62	41734.0
63	35896.5
64	30059.0
65	25576.0
66	21093.0
67	17768.0
68	14443.0
69	12946.5
70	11450.0
71	8937.0
72	6424.0
73	5515.0
74	4606.0
75	3597.0
76	2588.0
77	2268.0
78	1948.0
79	1601.5
80	1255.0
81	1023.0
82	791.0
83	611.5
84	432.0
85	314.0
86	196.0
87	151.5
88	107.0
89	76.0
90	45.0
91	35.5
92	26.0
93	21.5
94	17.0
95	15.0
96	13.0
97	10.0
98	7.0
99	7.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008393231278635343
2	2.098307819658836E-4
3	4.196615639317672E-4
4	0.0
5	0.0
6	2.098307819658836E-4
7	2.098307819658836E-4
8	0.0
9	0.0014688154737611853
10	6.294923458976509E-4
11	0.0
12	1.049153909829418E-4
13	6.294923458976509E-4
14	2.098307819658836E-4
15	0.004406446421283556
16	6.294923458976509E-4
17	0.0
18	2.098307819658836E-4
19	1.049153909829418E-4
20	6.294923458976509E-4
21	0.0
22	1.049153909829418E-4
23	6.294923458976509E-4
24	4.196615639317672E-4
25	0.0019933924286758943
26	0.004406446421283556
27	0.006924415804874159
28	0.004091700248334731
29	0.002727800165556487
30	0.0028327155565394287
31	0.006819500413891217
32	0.0030425463385053125
33	0.003881869466368847
34	0.0032523771204711963
35	0.0033572925114541377
36	0.006924415804874159
37	0.004091700248334731
38	0.0076588235417547525
39	0.004406446421283556
40	0.002727800165556487
41	0.0033572925114541377
42	9.442385188464763E-4
43	0.002098307819658836
44	0.0012589846917953017
45	0.0012589846917953017
46	9.442385188464763E-4
47	0.0016786462557270689
48	0.0019933924286758943
49	0.0
50	0.0014688154737611853
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	953149.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.337975435775725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.30849563880646	45.68359094989242
2	12.968009198167396	15.130548041072064
3	3.8186968588883694	6.683251306515108
4	1.6445838094184682	3.837667595037162
5	0.8465420440107462	2.469277448442514
6	0.5178837761700632	1.8127374607677549
7	0.35748380814649633	1.4598417132836503
8	0.2590278071552332	1.2088926280803878
9	0.19965412941720345	1.04826759278328
>10	0.9518317052581302	9.828007155595051
>50	0.06834104953303484	2.8506893853321236
>100	0.05473766131490534	6.233777323552893
>500	0.00471251371353905	1.7534513996455516
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.049153909829418E-4
6	0.0	0.0	0.0	0.0	4.196615639317672E-4
7	0.0	0.0	0.0	0.0	4.196615639317672E-4
8	0.0	0.0	0.0	0.0	4.196615639317672E-4
9	0.0	0.0	0.0	0.0	4.196615639317672E-4
10	0.0	0.0	0.0	0.0	4.196615639317672E-4
11	0.0	0.0	0.0	1.049153909829418E-4	4.196615639317672E-4
12	0.0	0.0	0.0	1.049153909829418E-4	5.245769549147091E-4
13	0.0	0.0	0.0	1.049153909829418E-4	7.344077368805926E-4
14	0.0	0.0	0.0	2.098307819658836E-4	7.344077368805926E-4
15	0.0	0.0	0.0	2.098307819658836E-4	8.393231278635344E-4
16	0.0	0.0	0.0	2.098307819658836E-4	8.393231278635344E-4
17	0.0	0.0	0.0	3.147461729488254E-4	9.442385188464762E-4
18	0.0	0.0	0.0	3.147461729488254E-4	9.442385188464762E-4
19	0.0	0.0	0.0	4.196615639317672E-4	9.442385188464762E-4
20	0.0	0.0	0.0	5.245769549147091E-4	0.0012589846917953017
21	0.0	0.0	0.0	7.344077368805926E-4	0.0012589846917953017
22	0.0	0.0	0.0	0.0011540693008123598	0.0012589846917953017
23	0.0	0.0	0.0	0.0019933924286758943	0.0012589846917953017
24	0.0	0.0	0.0	0.003252377120471196	0.0012589846917953017
25	0.0	0.0	0.0	0.003986784857351789	0.0012589846917953017
26	0.0	0.0	0.0	0.004826107985215323	0.0012589846917953017
27	0.0	0.0	0.0	0.005875261895044741	0.0012589846917953017
28	0.0	0.0	0.0	0.009337469797481821	0.0012589846917953017
29	0.0	0.0	0.0	0.014688154737611854	0.0012589846917953017
30	0.0	0.0	0.0	0.026228847745735452	0.0012589846917953017
31	0.0	0.0	0.0	0.05161837236360737	0.0012589846917953017
32	0.0	0.0	0.0	0.0787914586281893	0.0014688154737611853
33	0.0	0.0	0.0	0.1011384369075559	0.0014688154737611853
34	0.0	0.0	0.0	0.12317066901397368	0.0015737308647441272
35	0.0	0.0	0.0	0.15391087857197563	0.0015737308647441272
36	0.0	0.0	0.0	0.2015424660782312	0.0015737308647441272
37	0.0	0.0	0.0	0.26910797787124574	0.0015737308647441272
38	0.0	0.0	0.0	0.3637416605378592	0.0015737308647441272
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCG	260	0.0	20.305393	8
CTATACT	120	1.4322723E-8	20.164383	4
ATACCGT	275	0.0	19.197824	6
TACCGTC	275	0.0	19.197824	7
TCTATAC	105	1.79382E-6	18.855007	3
GTCTAGT	70	8.1306574E-4	18.854017	1
CGTCGTA	270	0.0	18.740583	10
TATACTC	130	3.5895937E-8	18.613277	5
ACCGCCT	95	1.5980906E-5	18.524218	8
CAATACT	95	1.5980906E-5	18.524218	4
TACGCTC	60	0.0074023693	18.337029	34
GATTTCG	120	3.1639865E-7	18.331257	41
GTGTAAG	180	1.4551915E-11	18.330296	1
GTATCAA	1895	0.0	18.22389	1
GTATAAT	145	6.315531E-9	18.20388	1
GTATAAA	145	6.315531E-9	18.20388	1
ACGCTAT	280	0.0	18.071276	10
ATTTCGT	110	2.796789E-6	17.998905	42
ATGGGTG	370	0.0	17.835817	5
TAATACC	100	2.4845367E-5	17.598007	4
>>END_MODULE