FastQCFastQC Report
Thu 2 Feb 2017
SRR4064033_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064033_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857599
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20130.2347250871327975No Hit
TATCAACGCAGAGTACTTTTTTTTT16340.19053193858668213No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16100.18773342786080674No Hit
GAATAGGACCGCGGTTCTATTTTGT10510.12255144887062602No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG10480.12220163502989159No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10000.11660461357814082No Hit
GATTAAGAGGGACGGCCGGGGGCAT9990.1164880089645627No Hit
GTCCTAAAGTGTGTATTTCTCATTT9830.11462233514731243No Hit
GTATCTGATCGTCTTCGAACCTCCG9550.1113574059671245No Hit
CTATTGGAGCTGGAATTACCGCGGC9430.1099581506041868No Hit
GCGCAAGACGGACCAGAGCGAAAGC9290.10832568601409284No Hit
GTCCTATTCCATTATTCCTAGCTGC9260.1079758721733584No Hit
GAATAACGCCGCCGCATCGCCAGTC9150.10669322142399887No Hit
GAACTACGACGGTATCTGATCGTCT9040.1054105706746393No Hit
GTCTTGCGCCGGTCCAAGAATTTCA8740.10191243226729509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGT250.006024354619.00303615
TTCGTAC307.710575E-419.00303517
CGCATCG1750.014.65862913
CATCGCC1850.014.38067515
GTACGAA400.00526891314.25310919
GCCGCGT400.005277081414.2497828
CGCGTAT400.005277081414.2497827
ATCGCCA2050.013.9046616
GCATCGC1850.013.8662714
GGGTCGC903.631976E-813.7220126
TCCTATA707.280889E-613.5664692
CCGCATC1900.013.501368512
CGCGTAA852.6947419E-713.41155810
TCGCGTA852.6947419E-713.4115589
TCGCCAG2000.013.30212517
CCGACCA1500.013.2997969
CTCCTAA500.001503383313.2943661
GCTCGTA801.996761E-613.06239
GCGTAAC801.996761E-613.062311
CCGTCAA1750.013.03141418
Produced by FastQC (version 0.11.5)