##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064033_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	857599
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.188905304227266	32.0	32.0	32.0	32.0	32.0
2	30.813357991322285	32.0	32.0	32.0	32.0	32.0
3	30.83567378226887	32.0	32.0	32.0	32.0	32.0
4	30.86457773388262	32.0	32.0	32.0	32.0	32.0
5	30.782569709153112	32.0	32.0	32.0	32.0	32.0
6	34.404384799888994	36.0	36.0	36.0	32.0	36.0
7	34.33658854546239	36.0	36.0	36.0	32.0	36.0
8	34.29023354738054	36.0	36.0	36.0	32.0	36.0
9	34.42332022308795	36.0	36.0	36.0	32.0	36.0
10	34.13943229877833	36.0	36.0	36.0	32.0	36.0
11	34.40319310073822	36.0	36.0	36.0	32.0	36.0
12	34.21950468692244	36.0	36.0	36.0	32.0	36.0
13	34.31057289012697	36.0	36.0	36.0	32.0	36.0
14	34.2167691426879	36.0	36.0	36.0	32.0	36.0
15	34.15015525904298	36.0	36.0	36.0	32.0	36.0
16	34.16004566236668	36.0	36.0	36.0	32.0	36.0
17	34.085703224933795	36.0	36.0	36.0	32.0	36.0
18	34.07619528474264	36.0	36.0	36.0	32.0	36.0
19	34.077500090368574	36.0	36.0	36.0	32.0	36.0
20	34.05489278788804	36.0	36.0	36.0	32.0	36.0
21	34.02768893154027	36.0	36.0	36.0	32.0	36.0
22	34.011528698144474	36.0	36.0	36.0	32.0	36.0
23	33.95822289904722	36.0	36.0	36.0	32.0	36.0
24	33.940461684307	36.0	36.0	36.0	32.0	36.0
25	33.55020469939914	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	6.0
5	8.0
6	36.0
7	14.0
8	45.0
9	60.0
10	75.0
11	24.0
12	45.0
13	51.0
14	84.0
15	162.0
16	251.0
17	320.0
18	406.0
19	613.0
20	861.0
21	1317.0
22	2040.0
23	3161.0
24	4692.0
25	6778.0
26	9846.0
27	13099.0
28	17679.0
29	24185.0
30	31793.0
31	43179.0
32	61161.0
33	86330.0
34	181722.0
35	367553.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.32361092965528	17.531588868773213	11.209849008006868	25.934951193564643
2	16.114418317192484	20.02215601331693	38.358589245004694	25.504836424485894
3	18.440323325419666	24.53334235087245	28.968105897004154	28.058228426703728
4	12.01681955938445	15.535120625299538	36.72177179085629	35.72628802445973
5	13.86739932036729	37.33361942900591	34.22384498590124	14.575136264725568
6	33.37049640692166	36.40566215967216	17.179019501283992	13.044821932122192
7	29.601270820236554	30.66693569738243	21.560207091447506	18.17158639093351
8	27.662239952408445	33.86010649776331	19.67794049889478	18.79971305093346
9	27.479214229634497	13.664303538159839	18.837676753418407	40.01880547878726
10	15.486756122004916	26.848385218751787	32.41754587784808	25.24731278139522
11	37.15864080501677	20.609012687764327	23.48560595012396	18.74674055709494
12	24.66063665194167	23.595933007096466	29.850206406657286	21.893223934304576
13	29.560270182918362	19.571343062065296	25.73295109147785	25.13543566353849
14	22.942565699043424	20.35357648766531	25.672276065501848	31.031581747789417
15	24.619197242702178	28.64453470015903	22.487168507626556	24.249099549512234
16	24.979609648749218	25.528953131946285	25.320907373547737	24.170529845756768
17	23.13645644944869	26.342570445131557	26.288431246718396	24.23254185870136
18	24.204451964874405	24.8379963240657	27.862298334159934	23.09525337689996
19	25.389598038677995	24.909886309136926	25.566149691997296	24.13436596018778
20	25.40345120133875	24.641885639132756	25.617590460708655	24.337072698819842
21	26.4807679292004	24.294419537104677	24.62057473329957	24.604237800395353
22	25.445513243159034	24.95165899784464	25.238845977456815	24.363981781539515
23	23.674705473896733	24.814298301405675	25.991819205611023	25.519177019086577
24	24.571079146429415	25.397762679742986	25.881472906392446	24.149685267435157
25	24.277557155343736	25.00738109436248	26.099666362865104	24.615395387428684
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	103.0
1	103.0
2	260.5
3	418.0
4	418.0
5	418.0
6	1323.0
7	2228.0
8	2228.0
9	2228.0
10	2156.0
11	2084.0
12	2084.0
13	2084.0
14	2230.0
15	2376.0
16	2376.0
17	2376.0
18	4101.0
19	5826.0
20	5826.0
21	5826.0
22	9958.5
23	14091.0
24	14091.0
25	14091.0
26	21766.5
27	29442.0
28	29442.0
29	29442.0
30	37388.0
31	45334.0
32	45334.0
33	45334.0
34	53410.0
35	61486.0
36	61486.0
37	61486.0
38	67709.5
39	73933.0
40	73933.0
41	73933.0
42	82726.5
43	91520.0
44	91520.0
45	91520.0
46	102250.0
47	112980.0
48	112980.0
49	112980.0
50	112072.5
51	111165.0
52	111165.0
53	111165.0
54	100964.5
55	90764.0
56	90764.0
57	90764.0
58	83095.0
59	75426.0
60	75426.0
61	75426.0
62	66868.5
63	58311.0
64	58311.0
65	58311.0
66	48229.0
67	38147.0
68	38147.0
69	38147.0
70	28765.0
71	19383.0
72	19383.0
73	19383.0
74	14939.5
75	10496.0
76	10496.0
77	10496.0
78	8624.5
79	6753.0
80	6753.0
81	6753.0
82	4657.0
83	2561.0
84	2561.0
85	2561.0
86	1993.0
87	1425.0
88	1425.0
89	1425.0
90	1025.5
91	626.0
92	626.0
93	626.0
94	433.0
95	240.0
96	240.0
97	240.0
98	360.5
99	481.0
100	481.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03906254554867718
2	0.005130602997438197
3	0.0012826507493595492
4	0.003031719953031662
5	0.008978555245516844
6	0.013642739788642477
7	0.023320922715628165
8	0.03544780252775481
9	0.046875054658412615
10	0.056669842198976444
11	0.05527058683603875
12	0.06448235130871188
13	0.06413253746797747
14	0.06821369894321239
15	0.06156723596925836
16	0.06716425742100912
17	0.06401593285439931
18	0.07859150955166692
19	0.07299448809991615
20	0.07929113723313577
21	0.07520997575790084
22	0.0776586726430418
23	0.08325569409479255
24	0.07940774184671391
25	0.07929113723313577
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	857599.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.266140758545596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.1787774716054	42.17547905892092
2	12.601399305691238	13.424558183431765
3	3.6915424391998917	5.899026575476984
4	1.4967030200974114	3.1889437496899604
5	0.7713934362735467	2.0544575678382455
6	0.46128511616104745	1.4742526756353844
7	0.3115174366925644	1.1615332142125214
8	0.21033638794382964	0.8963046117488067
9	0.15617763048468755	0.748708168386014
>10	0.9013622644449295	8.879469561877302
>50	0.10161560042050671	3.7697609323868617
>100	0.10623286818695667	11.421498291138386
>500	0.010557303666060686	4.0442109028452125
>1k	0.0010997191318813215	0.8617965064116276
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2013	0.2347250871327975	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1634	0.19053193858668213	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1610	0.18773342786080674	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1051	0.12255144887062602	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1048	0.12220163502989159	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1000	0.11660461357814082	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	999	0.1164880089645627	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	983	0.11462233514731243	No Hit
GTATCTGATCGTCTTCGAACCTCCG	955	0.1113574059671245	No Hit
CTATTGGAGCTGGAATTACCGCGGC	943	0.1099581506041868	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	929	0.10832568601409284	No Hit
GTCCTATTCCATTATTCCTAGCTGC	926	0.1079758721733584	No Hit
GAATAACGCCGCCGCATCGCCAGTC	915	0.10669322142399887	No Hit
GAACTACGACGGTATCTGATCGTCT	904	0.1054105706746393	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	874	0.10191243226729509	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1660461357814083E-4
2	0.0	0.0	0.0	0.0	1.1660461357814083E-4
3	0.0	0.0	0.0	0.0	1.1660461357814083E-4
4	0.0	0.0	0.0	0.0	1.1660461357814083E-4
5	0.0	0.0	0.0	0.0	1.1660461357814083E-4
6	0.0	0.0	0.0	0.0	1.1660461357814083E-4
7	0.0	0.0	0.0	0.0	1.1660461357814083E-4
8	0.0	0.0	0.0	0.0	1.1660461357814083E-4
9	0.0	0.0	0.0	0.0	1.1660461357814083E-4
10	0.0	0.0	0.0	0.0	1.1660461357814083E-4
11	0.0	0.0	0.0	1.1660461357814083E-4	1.1660461357814083E-4
12	0.0	1.1660461357814083E-4	0.0	1.1660461357814083E-4	5.830230678907041E-4
13	0.0	1.1660461357814083E-4	0.0	1.1660461357814083E-4	6.99627681468845E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTCGT	25	0.0060243546	19.003036	15
TTCGTAC	30	7.710575E-4	19.003035	17
CGCATCG	175	0.0	14.658629	13
CATCGCC	185	0.0	14.380675	15
GTACGAA	40	0.005268913	14.253109	19
GCCGCGT	40	0.0052770814	14.249782	8
CGCGTAT	40	0.0052770814	14.249782	7
ATCGCCA	205	0.0	13.90466	16
GCATCGC	185	0.0	13.86627	14
GGGTCGC	90	3.631976E-8	13.722012	6
TCCTATA	70	7.280889E-6	13.566469	2
CCGCATC	190	0.0	13.5013685	12
CGCGTAA	85	2.6947419E-7	13.411558	10
TCGCGTA	85	2.6947419E-7	13.411558	9
TCGCCAG	200	0.0	13.302125	17
CCGACCA	150	0.0	13.299796	9
CTCCTAA	50	0.0015033833	13.294366	1
GCTCGTA	80	1.996761E-6	13.0623	9
GCGTAAC	80	1.996761E-6	13.0623	11
CCGTCAA	175	0.0	13.031414	18
>>END_MODULE