FastQCFastQC Report
Thu 2 Feb 2017
SRR4064033_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064033_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857599
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10370.12091898428053204No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10290.11998614737190691No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10000.11660461357814082No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC10000.11660461357814082No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC9110.10622680296968628No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9000.10494415222032676No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA8820.10284526917592023No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8780.10237885072160766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG2450.021.5483061
GTCCTAC4850.020.4098011
GTCCTAA3550.019.828491
CCCGTCG1001.1227567E-619.79981641
TCCTACA6050.019.6338922
TTAGGAC4500.019.5530953
TAGGACA3200.019.2475764
TAGGACG5850.019.1770734
GATATAC2300.019.1280271
ATGTCGA708.1275724E-418.85476917
GAGTATA600.007415770618.3310281
CCTACAG6000.018.3310263
CGACGGT3150.018.1564457
GATATAA851.4315988E-418.1153661
CTATATA1102.797864E-617.9977341
TACTATA1102.797864E-617.9977342
AGGACGT6400.017.872755
TACGACG3200.017.872755
GTAGGAC6900.017.8528253
GGACGTG6200.017.73976
Produced by FastQC (version 0.11.5)