##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064033_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	857599
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.245836340760658	32.0	32.0	32.0	32.0	32.0
2	31.303128851596142	32.0	32.0	32.0	32.0	32.0
3	31.406354251812328	32.0	32.0	32.0	32.0	32.0
4	31.512860905854602	32.0	32.0	32.0	32.0	32.0
5	31.441732091571936	32.0	32.0	32.0	32.0	32.0
6	35.01516676208811	36.0	36.0	36.0	36.0	36.0
7	35.03120339459351	36.0	36.0	36.0	36.0	36.0
8	34.97413942880064	36.0	36.0	36.0	36.0	36.0
9	35.10212581871014	36.0	36.0	36.0	36.0	36.0
10	34.92843625050869	36.0	36.0	36.0	32.0	36.0
11	35.10071723497812	36.0	36.0	36.0	36.0	36.0
12	34.980879175465454	36.0	36.0	36.0	36.0	36.0
13	35.0494881640487	36.0	36.0	36.0	36.0	36.0
14	34.97727376081362	36.0	36.0	36.0	36.0	36.0
15	34.94563892914987	36.0	36.0	36.0	36.0	36.0
16	34.96135489896793	36.0	36.0	36.0	36.0	36.0
17	34.92954516038382	36.0	36.0	36.0	36.0	36.0
18	34.935516482645156	36.0	36.0	36.0	36.0	36.0
19	34.92260951796819	36.0	36.0	36.0	32.0	36.0
20	34.90268178950768	36.0	36.0	36.0	32.0	36.0
21	34.90830563002056	36.0	36.0	36.0	32.0	36.0
22	34.88108661507301	36.0	36.0	36.0	32.0	36.0
23	34.83599910914075	36.0	36.0	36.0	32.0	36.0
24	34.797624530812186	36.0	36.0	36.0	32.0	36.0
25	34.76639548320369	36.0	36.0	36.0	32.0	36.0
26	34.70518039316744	36.0	36.0	36.0	32.0	36.0
27	34.68970462885334	36.0	36.0	36.0	32.0	36.0
28	34.666454834952	36.0	36.0	36.0	32.0	36.0
29	34.625899750349525	36.0	36.0	36.0	32.0	36.0
30	34.60971852812328	36.0	36.0	36.0	32.0	36.0
31	34.59269308849474	36.0	36.0	36.0	32.0	36.0
32	34.55003445666331	36.0	36.0	36.0	32.0	36.0
33	34.52429049007753	36.0	36.0	36.0	32.0	36.0
34	34.51819789901807	36.0	36.0	36.0	32.0	36.0
35	34.46135781408327	36.0	36.0	36.0	32.0	36.0
36	34.437224157211006	36.0	36.0	36.0	32.0	36.0
37	34.41735006687274	36.0	36.0	36.0	32.0	36.0
38	34.357543560568516	36.0	36.0	36.0	32.0	36.0
39	34.33607198702424	36.0	36.0	36.0	32.0	36.0
40	34.32095769701224	36.0	36.0	36.0	32.0	36.0
41	34.297903798861704	36.0	36.0	36.0	32.0	36.0
42	34.209727390073915	36.0	36.0	36.0	32.0	36.0
43	34.21394614499317	36.0	36.0	36.0	32.0	36.0
44	34.12881778080432	36.0	36.0	36.0	32.0	36.0
45	34.085800006763066	36.0	36.0	36.0	32.0	36.0
46	34.06257819796898	36.0	36.0	36.0	32.0	36.0
47	34.01916979847225	36.0	36.0	36.0	32.0	36.0
48	33.967854440128775	36.0	36.0	36.0	32.0	36.0
49	33.94773081591746	36.0	36.0	36.0	32.0	36.0
50	33.3341480109002	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	3.0
20	7.0
21	32.0
22	91.0
23	300.0
24	782.0
25	1785.0
26	3653.0
27	6577.0
28	11253.0
29	17032.0
30	24385.0
31	34624.0
32	49705.0
33	79858.0
34	161182.0
35	466327.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.38165411710103	18.413985234387482	11.764561830273587	26.439798818237897
2	15.454111896380235	20.870059013732558	37.8096005466437	25.8662285432435
3	17.77520729385179	25.21423182629644	29.372119137265784	27.63844174258599
4	11.756310350175315	16.169678369494367	37.05624656745169	35.01776471287863
5	13.56169958220567	37.752026296672454	33.904074048593806	14.78220007252807
6	33.63802154389353	36.78028633462298	16.811606370350678	12.770085751132815
7	29.508299945428853	30.982187416918922	21.404250952663027	18.105261684989202
8	27.622117096685045	33.948617010980655	19.563922066140467	18.86534382619383
9	27.606399787311204	13.423415124459096	18.751596040536807	40.2185890476929
10	15.435365892053184	27.176304733386274	32.217414169258234	25.170915205302308
11	37.37632623172368	20.615928889842454	23.380507673166598	18.627237205267264
12	24.526847629253297	23.741865370645254	29.76869142804504	21.962595572056404
13	29.67096395039156	19.640133582712608	25.756766589396406	24.932135877499427
14	23.042354293789987	20.29888094552349	25.624680066091493	31.034084694595027
15	24.734304924891727	28.688188142664906	22.269876696961852	24.30763023548152
16	25.000932844522804	25.72563643317568	25.14365805651172	24.12977266578979
17	23.335265083098278	26.46971370069228	25.963766282376728	24.231254933832712
18	24.347973110850692	24.87409558124756	27.643351465435316	23.134579842466433
19	25.530113724479627	24.882841514507362	25.573957059185005	24.01308770182801
20	25.658035123969352	24.62805696421721	25.497235861850232	24.21667204996321
21	26.684616003516798	24.284426637624346	24.473792530075244	24.557164828783616
22	25.492013740703683	25.01218519632742	25.195371257862075	24.30042980510682
23	23.728977596703345	24.78984884508691	26.056177901741727	25.424995656468013
24	24.79573691544377	25.25959223176441	25.775336843148573	24.169334009643244
25	24.583393094312736	24.946506635509333	25.998066659437047	24.472033610740883
26	23.807369297470256	26.018764911527846	26.50747821138629	23.666387579615606
27	24.66307890548018	25.368464628678378	25.468401075162234	24.500055390679208
28	23.742690058479532	25.43130841393947	26.203028341874962	24.62297318570604
29	24.16161460683722	24.921668293354134	25.86561345941036	25.05110364039829
30	23.965213254225905	25.38515896115527	26.288527556998396	24.361100227620433
31	24.91612606802972	25.049414777438173	25.27762650474557	24.75683264978654
32	24.197996434114494	25.6399761652388	25.17995594533864	24.982071455308073
33	23.805936645462975	25.18054235588388	26.077627674026736	24.93589332462641
34	24.61816706196783	25.365041384183755	26.17803244967781	23.838759104170602
35	25.228319021609845	25.265517064456393	25.926919255759284	23.579244658174474
36	24.01241715965636	25.88222123488233	25.633131706088353	24.47222989937296
37	24.951548692689272	25.721055737077904	25.087515538235138	24.23988003199769
38	24.33813814584225	25.62180555377398	25.682327300205355	24.357729000178416
39	25.199926303048397	24.889979313545545	25.095327939773732	24.814766443632326
40	25.656325138937795	25.32842493627402	25.62495772979251	23.390292194995673
41	24.222190867440403	25.377316577441583	26.40265681245474	23.997835742663273
42	25.606327565559404	26.396573618118875	25.266534589735546	22.73056422658618
43	24.324084806280013	25.30801862920924	25.846745135719434	24.521151428791317
44	23.999631522976177	25.96468684117241	25.218870687885968	24.816810947965447
45	24.28575259946291	26.110186676243995	25.256039954243388	24.34802077004971
46	24.15571149715597	25.37634709521844	25.382760446182655	25.085180961442934
47	24.85097600223886	25.25397047505772	25.72296462137643	24.17208890132699
48	25.261491029429255	25.89769841251332	24.471129291426358	24.369681266631062
49	24.389572712559293	26.022742643389197	24.949743235742705	24.63794140830881
50	24.395835301059133	26.535321698389318	24.48818723187914	24.580655768672404
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	23.5
2	24.0
3	71.5
4	119.0
5	133.5
6	148.0
7	153.5
8	159.0
9	220.0
10	281.0
11	403.5
12	526.0
13	949.5
14	1373.0
15	1833.5
16	2294.0
17	2570.0
18	2846.0
19	2897.0
20	2948.0
21	3383.5
22	3819.0
23	4248.5
24	4678.0
25	5474.0
26	6270.0
27	8183.5
28	10097.0
29	11843.5
30	13590.0
31	15347.0
32	17104.0
33	20567.0
34	24030.0
35	28235.5
36	32441.0
37	35859.0
38	39277.0
39	40510.5
40	41744.0
41	43846.5
42	45949.0
43	45611.5
44	45274.0
45	49671.0
46	54068.0
47	57430.0
48	60792.0
49	62695.0
50	64598.0
51	61057.0
52	57516.0
53	56409.5
54	55303.0
55	55887.0
56	56471.0
57	54932.5
58	53394.0
59	48742.5
60	44091.0
61	38776.0
62	33461.0
63	29032.5
64	24604.0
65	21010.0
66	17416.0
67	14935.5
68	12455.0
69	11489.5
70	10524.0
71	8282.0
72	6040.0
73	5232.0
74	4424.0
75	3479.5
76	2535.0
77	2195.5
78	1856.0
79	1560.0
80	1264.0
81	998.5
82	733.0
83	650.0
84	567.0
85	398.0
86	229.0
87	166.0
88	103.0
89	73.5
90	44.0
91	39.5
92	35.0
93	24.5
94	14.0
95	17.0
96	20.0
97	16.0
98	12.0
99	14.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007695904496157295
2	2.3320922715628167E-4
3	0.0
4	0.0
5	0.0
6	1.1660461357814083E-4
7	3.498138407344225E-4
8	0.0
9	9.328369086251267E-4
10	0.0011660461357814085
11	0.0
12	0.0
13	3.498138407344225E-4
14	0.0
15	0.004314370702391211
16	8.162322950469858E-4
17	0.0
18	4.6641845431256334E-4
19	0.0
20	9.328369086251267E-4
21	0.0
22	1.1660461357814083E-4
23	2.3320922715628167E-4
24	4.6641845431256334E-4
25	0.0018656738172502533
26	0.004780789156703774
27	0.006296649133219604
28	0.003964556861656789
29	0.0032649291801879433
30	0.003614743020922366
31	0.007229486041844732
32	0.0032649291801879433
33	0.005130602997438197
34	0.0029151153394535207
35	0.003148324566609802
36	0.008861950631938703
37	0.004314370702391211
38	0.006529858360375887
39	0.0038479522480786474
40	0.0024486968851409573
41	0.003498138407344225
42	0.002098883044406535
43	0.0024486968851409573
44	0.0017490692036721124
45	0.002098883044406535
46	0.001515859976515831
47	0.002215487657984676
48	0.0019822784308283943
49	3.498138407344225E-4
50	0.0011660461357814085
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	857599.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.64641118412601
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.10170653893029	46.175759116933705
2	12.396296795126183	14.29204044424615
3	3.4725915092303072	6.005473140467851
4	1.3409333612603143	3.091999836548971
5	0.7091305421818243	2.0439415408917827
6	0.41962254550913086	1.4513840280329395
7	0.27573614576159405	1.112663946582927
8	0.1972837658502893	0.9098160868926891
9	0.1518871356785864	0.7880173449216235
>10	0.7581302888181759	8.070206937413204
>50	0.0858006070094094	3.451932266881156
>100	0.08356150844545261	9.555282087797066
>500	0.006912630854151569	2.809342942106608
>1k	4.06625344361857E-4	0.24214028028336468
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1037	0.12091898428053204	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1029	0.11998614737190691	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1000	0.11660461357814082	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1000	0.11660461357814082	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	911	0.10622680296968628	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	900	0.10494415222032676	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	882	0.10284526917592023	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	878	0.10237885072160766	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	1.1660461357814083E-4	0.0	0.0	0.0
10	0.0	1.1660461357814083E-4	0.0	0.0	0.0
11	0.0	1.1660461357814083E-4	0.0	1.1660461357814083E-4	0.0
12	0.0	1.1660461357814083E-4	0.0	1.1660461357814083E-4	0.0
13	0.0	1.1660461357814083E-4	0.0	1.1660461357814083E-4	0.0
14	0.0	1.1660461357814083E-4	0.0	1.1660461357814083E-4	0.0
15	0.0	1.1660461357814083E-4	0.0	1.1660461357814083E-4	1.1660461357814083E-4
16	0.0	1.1660461357814083E-4	0.0	1.1660461357814083E-4	1.1660461357814083E-4
17	0.0	1.1660461357814083E-4	0.0	1.1660461357814083E-4	1.1660461357814083E-4
18	0.0	1.1660461357814083E-4	0.0	1.1660461357814083E-4	1.1660461357814083E-4
19	0.0	1.1660461357814083E-4	0.0	1.1660461357814083E-4	1.1660461357814083E-4
20	0.0	1.1660461357814083E-4	0.0	3.498138407344225E-4	2.3320922715628167E-4
21	0.0	1.1660461357814083E-4	0.0	3.498138407344225E-4	2.3320922715628167E-4
22	0.0	1.1660461357814083E-4	0.0	6.99627681468845E-4	2.3320922715628167E-4
23	0.0	1.1660461357814083E-4	0.0	0.0011660461357814083	2.3320922715628167E-4
24	0.0	1.1660461357814083E-4	0.0	0.0016324645900939717	2.3320922715628167E-4
25	0.0	1.1660461357814083E-4	0.0	0.0019822784308283943	2.3320922715628167E-4
26	0.0	1.1660461357814083E-4	0.0	0.0025653014987190984	2.3320922715628167E-4
27	0.0	1.1660461357814083E-4	0.0	0.0037313476345005067	2.3320922715628167E-4
28	0.0	1.1660461357814083E-4	0.0	0.005363812224594478	2.3320922715628167E-4
29	0.0	1.1660461357814083E-4	0.0	0.00979478754056383	2.3320922715628167E-4
30	0.0	1.1660461357814083E-4	0.0	0.01539180899231459	2.3320922715628167E-4
31	0.0	1.1660461357814083E-4	0.0	0.028334921099488222	2.3320922715628167E-4
32	0.0	1.1660461357814083E-4	0.0	0.04419314854611538	2.3320922715628167E-4
33	0.0	1.1660461357814083E-4	0.0	0.05970156215200811	2.3320922715628167E-4
34	0.0	1.1660461357814083E-4	0.0	0.07590960343936969	2.3320922715628167E-4
35	0.0	1.1660461357814083E-4	0.0	0.0947995508390285	2.3320922715628167E-4
36	0.0	1.1660461357814083E-4	0.0	0.12465033191503255	2.3320922715628167E-4
37	0.0	1.1660461357814083E-4	0.0	0.16569515589453812	2.3320922715628167E-4
38	0.0	1.1660461357814083E-4	0.0	0.2230646257749834	2.3320922715628167E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	245	0.0	21.548306	1
GTCCTAC	485	0.0	20.409801	1
GTCCTAA	355	0.0	19.82849	1
CCCGTCG	100	1.1227567E-6	19.799816	41
TCCTACA	605	0.0	19.633892	2
TTAGGAC	450	0.0	19.553095	3
TAGGACA	320	0.0	19.247576	4
TAGGACG	585	0.0	19.177073	4
GATATAC	230	0.0	19.128027	1
ATGTCGA	70	8.1275724E-4	18.854769	17
GAGTATA	60	0.0074157706	18.331028	1
CCTACAG	600	0.0	18.331026	3
CGACGGT	315	0.0	18.156445	7
GATATAA	85	1.4315988E-4	18.115366	1
CTATATA	110	2.797864E-6	17.997734	1
TACTATA	110	2.797864E-6	17.997734	2
AGGACGT	640	0.0	17.87275	5
TACGACG	320	0.0	17.87275	5
GTAGGAC	690	0.0	17.852825	3
GGACGTG	620	0.0	17.7397	6
>>END_MODULE