FastQCFastQC Report
Thu 2 Feb 2017
SRR4064032_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064032_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339864
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39920.29794068651743755No Hit
TATCAACGCAGAGTACTTTTTTTTT28260.21091692888233435No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23160.17285336422204045No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15940.11896729817354598No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15910.11874339485201484No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG15240.11374288733781936No Hit
GCGCAAGACGGACCAGAGCGAAAGC14800.11045897195536264No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14710.10978726199076921No Hit
GAATAACGCCGCCGCATCGCCAGTC14640.10926482090719655No Hit
GAATAGGACCGCGGTTCTATTTTGT14470.1079960354185201No Hit
GTATCTGATCGTCTTCGAACCTCCG14150.10560773332218792No Hit
GAACTACGACGGTATCTGATCGTCT14140.10553309888167754No Hit
GTACATGGGGTGGTATCAACGCAAA14100.10523456111963601No Hit
GTCCTATTCCATTATTCCTAGCTGC13400.10001015028390942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGGT456.754108E-414.779915
CGCCAGT2050.013.90495518
CGCCCGA551.9580676E-413.81964916
CGAACGA1051.36788E-913.57286916
TTCGGTA500.001499002913.30041812
ACGAACG1102.739398E-912.95640515
CGGTCCA2350.012.93754410
TCGCGTA1251.0186341E-1012.9213729
CGCATCG2300.012.80475113
CCCTTAA751.4811512E-512.66422751
TTAGGAC1950.012.6632823
AAATCGC1301.9645086E-1012.424396514
CGCGTAA1301.9645086E-1012.424396510
CCGCATC2300.012.39169412
ATTGATC701.0892225E-412.21603915
GTCCTAA1405.2750693E-1112.2119331
ATTAGTC550.00307421212.0876773
AATCGCT1353.6925485E-1011.9646815
GTCGTAG1750.011.94457111
CGACGCT802.8614571E-511.87670515
Produced by FastQC (version 0.11.5)