##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064032_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1339864
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22790596657571	32.0	32.0	32.0	32.0	32.0
2	30.88961715517396	32.0	32.0	32.0	32.0	32.0
3	30.914662980720433	32.0	32.0	32.0	32.0	32.0
4	30.939092325788287	32.0	32.0	32.0	32.0	32.0
5	30.86795600150463	32.0	32.0	32.0	32.0	32.0
6	34.5139947039401	36.0	36.0	36.0	32.0	36.0
7	34.440321555023495	36.0	36.0	36.0	32.0	36.0
8	34.3981627986124	36.0	36.0	36.0	32.0	36.0
9	34.51599117522375	36.0	36.0	36.0	32.0	36.0
10	34.25754703462441	36.0	36.0	36.0	32.0	36.0
11	34.4974997462429	36.0	36.0	36.0	32.0	36.0
12	34.32771908193667	36.0	36.0	36.0	32.0	36.0
13	34.40339168751456	36.0	36.0	36.0	32.0	36.0
14	34.32505687144367	36.0	36.0	36.0	32.0	36.0
15	34.26604789739854	36.0	36.0	36.0	32.0	36.0
16	34.263061027089314	36.0	36.0	36.0	32.0	36.0
17	34.19887988631682	36.0	36.0	36.0	32.0	36.0
18	34.18037278410346	36.0	36.0	36.0	32.0	36.0
19	34.19777305756405	36.0	36.0	36.0	32.0	36.0
20	34.16550858893142	36.0	36.0	36.0	32.0	36.0
21	34.154457467325045	36.0	36.0	36.0	32.0	36.0
22	34.1290511574309	36.0	36.0	36.0	32.0	36.0
23	34.08094105073351	36.0	36.0	36.0	32.0	36.0
24	34.071414710746765	36.0	36.0	36.0	32.0	36.0
25	33.67746129457915	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	7.0
5	14.0
6	60.0
7	23.0
8	81.0
9	95.0
10	126.0
11	33.0
12	89.0
13	89.0
14	130.0
15	235.0
16	425.0
17	545.0
18	687.0
19	905.0
20	1309.0
21	1933.0
22	2972.0
23	4506.0
24	6913.0
25	10009.0
26	14277.0
27	19095.0
28	25977.0
29	34764.0
30	46588.0
31	63200.0
32	88966.0
33	125900.0
34	272858.0
35	617052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.627241294091384	17.147061744175716	11.265995644152495	25.959701317580407
2	16.61106110342336	19.950231154938745	37.59660067144651	25.84210707019139
3	18.861122845461345	23.969097941106664	28.731510934838777	28.43826827859321
4	12.369543556386516	15.28248302937346	36.34554024249616	36.00243317174386
5	14.30654994715377	36.89532863190957	33.728659974801005	15.069461446135657
6	33.71461841633823	36.005314702018396	17.02473725068673	13.255329630956647
7	29.87064274015226	30.693160219955118	20.91268235655961	18.52351468333301
8	28.030942744275883	32.92082811635559	19.641295915937036	19.40693322343149
9	27.389738826031373	13.936173072557617	18.673759543124767	40.00032855828624
10	15.498792085640567	26.89978604793572	31.83173584947931	25.769686016944405
11	37.364601123591314	21.1363760259697	22.46674816425074	19.03227468618825
12	24.445563048042114	23.771687362907784	29.315944856161934	22.466804732888164
13	29.453636473626588	19.500404044326874	25.63829294632708	25.407666535719457
14	23.434317633377923	19.889298920548708	25.53593527922857	31.1404481668448
15	24.946416595844468	27.520287012876448	22.654615192743425	24.87868119853566
16	25.375709332946954	25.920471061306984	24.320392192595005	24.38342741315106
17	23.599024941075253	25.97812092045085	25.687828791124094	24.735025347349808
18	24.28825921817754	25.294462557747828	26.771686479259333	23.6455917448153
19	25.213618396876093	25.10202892661984	25.837369177404852	23.846983499099217
20	25.417063176089027	24.46529117112326	25.458818045872096	24.658827606915622
21	26.603720877426756	24.33053186976033	24.741041391549597	24.32470586126332
22	25.459199564982594	24.66677173611832	25.363143180034015	24.51088551886507
23	24.133513506649308	24.427303577844658	25.734232407143885	25.704950508362153
24	24.31022917914695	25.34893761325627	25.73593232036977	24.604900887227007
25	24.463986399476532	24.8851186012256	25.803945705467395	24.846949293830463
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	118.0
1	118.0
2	354.5
3	591.0
4	591.0
5	591.0
6	1895.5
7	3200.0
8	3200.0
9	3200.0
10	3219.5
11	3239.0
12	3239.0
13	3239.0
14	3542.0
15	3845.0
16	3845.0
17	3845.0
18	6794.0
19	9743.0
20	9743.0
21	9743.0
22	15553.0
23	21363.0
24	21363.0
25	21363.0
26	31788.5
27	42214.0
28	42214.0
29	42214.0
30	53542.0
31	64870.0
32	64870.0
33	64870.0
34	77332.0
35	89794.0
36	89794.0
37	89794.0
38	100413.5
39	111033.0
40	111033.0
41	111033.0
42	126376.0
43	141719.0
44	141719.0
45	141719.0
46	157867.0
47	174015.0
48	174015.0
49	174015.0
50	176175.5
51	178336.0
52	178336.0
53	178336.0
54	163013.0
55	147690.0
56	147690.0
57	147690.0
58	134605.0
59	121520.0
60	121520.0
61	121520.0
62	107819.0
63	94118.0
64	94118.0
65	94118.0
66	78004.5
67	61891.0
68	61891.0
69	61891.0
70	47006.5
71	32122.0
72	32122.0
73	32122.0
74	25104.5
75	18087.0
76	18087.0
77	18087.0
78	14693.0
79	11299.0
80	11299.0
81	11299.0
82	7927.5
83	4556.0
84	4556.0
85	4556.0
86	3506.5
87	2457.0
88	2457.0
89	2457.0
90	1716.5
91	976.0
92	976.0
93	976.0
94	656.0
95	336.0
96	336.0
97	336.0
98	534.0
99	732.0
100	732.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03843673686284578
2	0.005224410835726611
3	0.001343419929186843
4	0.0036570875850086273
5	0.00955320838532866
6	0.013732737053909948
7	0.023733752082300892
8	0.036346972528555135
9	0.05075141954705851
10	0.05814022915758614
11	0.05709534699044082
12	0.06664855537576948
13	0.06732026534036291
14	0.0703802774012885
15	0.06194658562361553
16	0.06941002967465354
17	0.06478269436300997
18	0.0797842169055964
19	0.0767242048446708
20	0.08194861568039741
21	0.07761981813079537
22	0.07963494802457563
23	0.08605350990846833
24	0.08105300239427285
25	0.08150080903733514
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1339864.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.46599046314093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.61889697466273	38.16182533556566
2	14.247406510705385	14.380189621874997
3	4.561034591390603	6.905313845735223
4	1.9695674636209006	3.975846913424201
5	0.9872142286117734	2.4910371923099395
6	0.5736977509687442	1.7371335137468427
7	0.3610126367676443	1.2753202198932507
8	0.24710528312539645	0.9976330289278399
9	0.17944905195752264	0.8150466730237218
>10	1.0513456897906115	9.724520215943528
>50	0.09558984696581882	3.332543209804515
>100	0.09355071119584607	9.870155662454492
>500	0.0104110338588897	3.4852992276979218
>1k	0.003718226378174893	2.848135339597832
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3992	0.29794068651743755	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2826	0.21091692888233435	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2316	0.17285336422204045	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1594	0.11896729817354598	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1591	0.11874339485201484	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1524	0.11374288733781936	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1480	0.11045897195536264	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1471	0.10978726199076921	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1464	0.10926482090719655	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1447	0.1079960354185201	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1415	0.10560773332218792	No Hit
GAACTACGACGGTATCTGATCGTCT	1414	0.10553309888167754	No Hit
GTACATGGGGTGGTATCAACGCAAA	1410	0.10523456111963601	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1340	0.10001015028390942	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	7.463444051038016E-5	0.0	0.0	0.0
6	0.0	7.463444051038016E-5	0.0	0.0	0.0
7	0.0	7.463444051038016E-5	0.0	7.463444051038016E-5	0.0
8	0.0	7.463444051038016E-5	0.0	7.463444051038016E-5	0.0
9	0.0	7.463444051038016E-5	0.0	7.463444051038016E-5	0.0
10	0.0	7.463444051038016E-5	0.0	7.463444051038016E-5	0.0
11	0.0	7.463444051038016E-5	7.463444051038016E-5	7.463444051038016E-5	0.0
12	0.0	7.463444051038016E-5	7.463444051038016E-5	7.463444051038016E-5	0.0
13	0.0	7.463444051038016E-5	7.463444051038016E-5	7.463444051038016E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTGGT	45	6.754108E-4	14.7799	15
CGCCAGT	205	0.0	13.904955	18
CGCCCGA	55	1.9580676E-4	13.819649	16
CGAACGA	105	1.36788E-9	13.572869	16
TTCGGTA	50	0.0014990029	13.300418	12
ACGAACG	110	2.739398E-9	12.956405	15
CGGTCCA	235	0.0	12.937544	10
TCGCGTA	125	1.0186341E-10	12.921372	9
CGCATCG	230	0.0	12.804751	13
CCCTTAA	75	1.4811512E-5	12.6642275	1
TTAGGAC	195	0.0	12.663282	3
AAATCGC	130	1.9645086E-10	12.4243965	14
CGCGTAA	130	1.9645086E-10	12.4243965	10
CCGCATC	230	0.0	12.391694	12
ATTGATC	70	1.0892225E-4	12.216039	15
GTCCTAA	140	5.2750693E-11	12.211933	1
ATTAGTC	55	0.003074212	12.087677	3
AATCGCT	135	3.6925485E-10	11.96468	15
GTCGTAG	175	0.0	11.944571	11
CGACGCT	80	2.8614571E-5	11.876705	15
>>END_MODULE