FastQCFastQC Report
Thu 2 Feb 2017
SRR4064032_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064032_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339864
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA15060.1123994674086325No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15060.1123994674086325No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14760.11016043419332111No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT14390.10739895989443704No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14070.10501065779810487No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA13680.10209991461820006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG609.759335E-625.664865
TGTAGGA4550.019.822782
TAGGACG3350.019.0434364
TTAACGG1303.5848643E-818.61685235
TAACACG600.00741552118.3320434
GGCGTTA3650.018.08294342
GTAGGAC4500.017.598763
TACGACG4500.017.598765
GTATCAA33100.017.3455641
GTCCTAC4600.017.2155361
CGAAAAC4150.016.96392823
TAATACT2350.016.8498784
GTCTAAA1702.4720066E-916.8217161
CGTCTTA1602.1305823E-816.49945415
TGCGTAC800.001989437716.49883816
ATTGTCG1753.623427E-916.34353643
CGACGGT4750.016.2093857
GTATTAG3800.016.208781
ACGACGG4900.016.1621276
AACCGCG3950.016.15256941
Produced by FastQC (version 0.11.5)