FastQCFastQC Report
Thu 2 Feb 2017
SRR4064031_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064031_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927795
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT64830.6987534961925856No Hit
CTGTAGGACGTGGAATATGGCAAGA56040.6040127398832716No Hit
GTCCTACAGTGGACATTTCTAAATT54910.591833325249651No Hit
CTTTAGGACGTGAAATATGGCGAGG51070.5504448719814183No Hit
GTCCTACAGTGTGCATTTCTCATTT37380.4028907247829531No Hit
GTATCAACGCAGAGTACTTTTTTTT28690.30922779277749934No Hit
ATTTAGAAATGTCCACTGTAGGACG27380.29510829439693037No Hit
CTGTAGGACCTGGAATATGGCGAGA26160.2819588378898356No Hit
CTGAAGGACCTGGAATATGGCGAGA25610.2760308042186043No Hit
GTCCTTCAGTGTGCATTTCTCATTT23840.25695331404027827No Hit
TTTCTAAATTTTCCACCTTTTTCAG20240.21815163910131008No Hit
TATCAACGCAGAGTACTTTTTTTTT18950.20424770558151317No Hit
GAATATGGCAAGAAAACTGAAAATC17000.18323013165623872No Hit
GGAATATGGCGAGAAAACTGAAAAT13890.14970979580618562No Hit
GCCATATTCCACGTCCTACAGTGGA13250.14281172026148017No Hit
CCATATTCCAGGTCCTTCAGTGTGC13140.14162611352723392No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12910.1391471176283554No Hit
GATATACACTGTTCTACAAATCCCG12820.1381770757548812No Hit
ATTCCAGGTCCTTCAGTGTGCATTT12110.1305245231974736No Hit
TTCCAGGTCCTTCAGTGTGCATTTC11920.12847665702013913No Hit
GAAATATGGCGAGGAAAACTGAAAA11640.1254587489693305No Hit
TTGTAGAACAGTGTATATCAATGAG11340.12222527605774981No Hit
CTGTAGGACATGGAATATGGCAAGA11110.1197462801588713No Hit
GACCTGGAATATGGCGAGAAAACTG10710.11543498294343038No Hit
GTCCACTGTAGGACGTGGAATATGG10700.11532720051304438No Hit
ACAGTGGACATTTCTAAATTTTCCA10600.11424937620918413No Hit
CCATATTTCACGTCCTAAAGTGTGT10580.1140338113484121No Hit
ACCTGGAATATGGCGAGAAAACTGA10190.1098302965633572No Hit
ATACACACTTTAGGACGTGAAATAT9700.10454895747444208No Hit
CCTAAAGTGTGTATTTCTCATTTTC9590.10336335074019586No Hit
CCACTGTAGGACGTGGAATATGGCA9490.10228552643633561No Hit
GTGTATATCAATGAGTTACAATGAG9430.10163883185401947No Hit
GGTATCAACGCAGAGTACTTTTTTT9380.10109991970208936No Hit
CACTTTAGGACGTGAAATATGGCGA9330.10056100755015925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT453.5310695E-516.89186119
GTCGAGA508.694841E-515.20267519
TCCAACG1700.015.09089118
AATGTCC8050.014.3969438
TGTCGAG1006.566552E-1014.25250818
TATTGCG400.00527270814.25173916
CTACCGT400.00528592814.2463624
TGTCCAC8200.013.78752410
AAATGTC8200.013.7860377
CCAACGA1400.013.57381719
TAGGACC9650.013.2867624
CTAGGAC1600.013.0577563
GGCGAGG13400.013.04707119
ATGTCCA8800.012.9554279
TAGAAAT8750.012.91674
GTCCTAT1850.012.8400971
ATAGGAC2150.012.8093013
AATGGCG604.085053E-412.66821316
TCTACAC1207.421477E-1012.6620673
ATGTCGA1155.296897E-912.39348517
Produced by FastQC (version 0.11.5)