##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064031_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	927795
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35028643180875	32.0	32.0	32.0	32.0	32.0
2	31.047134334632112	32.0	32.0	32.0	32.0	32.0
3	31.07164837059911	32.0	32.0	32.0	32.0	32.0
4	31.071322867659344	32.0	32.0	32.0	32.0	32.0
5	31.0738234200443	32.0	32.0	32.0	32.0	32.0
6	34.744968446693505	36.0	36.0	36.0	32.0	36.0
7	34.67843650806482	36.0	36.0	36.0	32.0	36.0
8	34.64543029440771	36.0	36.0	36.0	32.0	36.0
9	34.70833750990251	36.0	36.0	36.0	32.0	36.0
10	34.501720746501114	36.0	36.0	36.0	32.0	36.0
11	34.713178018851146	36.0	36.0	36.0	32.0	36.0
12	34.57290349700095	36.0	36.0	36.0	32.0	36.0
13	34.60976401036867	36.0	36.0	36.0	32.0	36.0
14	34.56851890773285	36.0	36.0	36.0	32.0	36.0
15	34.5558835734187	36.0	36.0	36.0	32.0	36.0
16	34.53788067407132	36.0	36.0	36.0	32.0	36.0
17	34.509572696554734	36.0	36.0	36.0	32.0	36.0
18	34.518936834106675	36.0	36.0	36.0	32.0	36.0
19	34.45691235671673	36.0	36.0	36.0	32.0	36.0
20	34.41546246746318	36.0	36.0	36.0	32.0	36.0
21	34.400873037686125	36.0	36.0	36.0	32.0	36.0
22	34.3574194730517	36.0	36.0	36.0	32.0	36.0
23	34.36055809742454	36.0	36.0	36.0	32.0	36.0
24	34.340595713492746	36.0	36.0	36.0	32.0	36.0
25	33.98905361636999	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	11.0
5	4.0
6	33.0
7	15.0
8	46.0
9	74.0
10	72.0
11	22.0
12	57.0
13	43.0
14	90.0
15	156.0
16	285.0
17	343.0
18	396.0
19	471.0
20	724.0
21	952.0
22	1545.0
23	2309.0
24	3387.0
25	5165.0
26	7696.0
27	10266.0
28	14638.0
29	20377.0
30	27557.0
31	38486.0
32	54960.0
33	80901.0
34	187219.0
35	469493.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.71099123500306	21.03814913972779	12.639214007605892	24.611645617663264
2	11.265644183020909	21.718453749483963	48.15665147923828	18.859250588256852
3	17.471687822880615	28.645229854890324	30.89737772168216	22.985704600546896
4	9.855512980118888	18.2171131698221	41.983153061389466	29.944220788669547
5	9.62322846018859	41.812435595069225	37.7215637409402	10.842772203801992
6	27.28480033632106	41.45954715232002	20.3261883074535	10.92946420390542
7	25.109827736206448	33.422884488712135	24.505573050133194	16.961714724948227
8	21.76844343381087	43.089002243735926	21.43031965419943	13.712234668253778
9	27.094781701015364	14.863935161621477	21.94970194704923	36.091581190313924
10	13.804263193879773	30.825225442851504	36.91870684988234	18.451804513386378
11	32.38192192509563	22.3483725138549	28.973060178649806	16.29664538239966
12	24.232521771953948	25.264957264957268	35.27212920272857	15.230391760360215
13	29.414981158691916	24.74649974439668	26.90197990116672	18.93653919574469
14	18.0917806401931	25.743295056347737	29.614134766482348	26.550789536976822
15	19.174020937428217	39.00375199921488	23.821366443818693	18.00086061953822
16	17.33111729951617	29.621260982201985	34.592561115293435	18.45506060298841
17	17.496675470961463	32.45398237055907	31.62956388003007	18.419778278449396
18	17.630993356624877	28.841243936666274	37.235907232207474	16.291855474501368
19	22.556536145812885	26.961857651660114	29.48292859716149	20.998677605365515
20	23.157390608382	29.544851480235835	30.949150129114795	16.348607782267372
21	20.707586040270773	28.015993545631435	28.232038001389242	23.044382412708547
22	21.823973180481637	32.93329852893906	28.776726215259423	16.466002075319874
23	18.206314499574454	32.567060140813616	31.351903529617353	17.874721829994574
24	22.031155954515157	28.21833761560516	32.50104090486453	17.249465525015157
25	19.716225457730452	30.826449668527	31.874262464485035	17.583062409257515
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	87.0
1	87.0
2	253.5
3	420.0
4	420.0
5	420.0
6	1192.0
7	1964.0
8	1964.0
9	1964.0
10	2409.5
11	2855.0
12	2855.0
13	2855.0
14	3961.0
15	5067.0
16	5067.0
17	5067.0
18	9559.0
19	14051.0
20	14051.0
21	14051.0
22	24248.0
23	34445.0
24	34445.0
25	34445.0
26	53262.5
27	72080.0
28	72080.0
29	72080.0
30	96406.5
31	120733.0
32	120733.0
33	120733.0
34	128888.0
35	137043.0
36	137043.0
37	137043.0
38	138324.0
39	139605.0
40	139605.0
41	139605.0
42	134433.0
43	129261.0
44	129261.0
45	129261.0
46	122441.0
47	115621.0
48	115621.0
49	115621.0
50	98784.0
51	81947.0
52	81947.0
53	81947.0
54	60002.0
55	38057.0
56	38057.0
57	38057.0
58	28447.5
59	18838.0
60	18838.0
61	18838.0
62	13740.5
63	8643.0
64	8643.0
65	8643.0
66	6257.5
67	3872.0
68	3872.0
69	3872.0
70	2720.5
71	1569.0
72	1569.0
73	1569.0
74	1144.5
75	720.0
76	720.0
77	720.0
78	509.0
79	298.0
80	298.0
81	298.0
82	211.5
83	125.0
84	125.0
85	125.0
86	96.0
87	67.0
88	67.0
89	67.0
90	49.5
91	32.0
92	32.0
93	32.0
94	39.5
95	47.0
96	47.0
97	47.0
98	197.5
99	348.0
100	348.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038370545217424105
2	0.00582025124084523
3	0.001508954025404319
4	0.003233472911580683
5	0.0076525525574076166
6	0.011856067342462506
7	0.02220318065952069
8	0.03492150744507137
9	0.04774761666100809
10	0.05486125706648559
11	0.05453790977532752
12	0.06143598532003298
13	0.06219046233273514
14	0.0653161528139298
15	0.05992703129462867
16	0.06607062982663196
17	0.06423832851006957
18	0.07609439585253208
19	0.07426209453596969
20	0.07684887286523423
21	0.07232201078902128
22	0.07641774314369015
23	0.08126795251106117
24	0.07728000258677833
25	0.07771113230832241
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	927795.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.709361406142826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.24824536498167	48.287419621538206
2	11.942850756257977	14.023142825375512
3	2.85279627303391	5.024575422349353
4	0.9991384333684803	2.346351175175898
5	0.4653787722603572	1.3661045265690173
6	0.28702805843102847	1.0110740409678436
7	0.19034210624203243	0.782240445431894
8	0.13750294633179105	0.6458168136482064
9	0.09600262630785363	0.5072627595461988
>10	0.6507634223180817	7.337967019126765
>50	0.06128603488058411	2.4549974908220653
>100	0.05722100473976277	6.722068306999901
>500	0.006275852077026119	2.674522731579278
>1k	0.004430013230841967	4.358128289027239
>5k	7.383355384736612E-4	2.4583285318426227
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	6483	0.6987534961925856	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5604	0.6040127398832716	No Hit
GTCCTACAGTGGACATTTCTAAATT	5491	0.591833325249651	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5107	0.5504448719814183	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3738	0.4028907247829531	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2869	0.30922779277749934	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2738	0.29510829439693037	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2616	0.2819588378898356	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2561	0.2760308042186043	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2384	0.25695331404027827	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2024	0.21815163910131008	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1895	0.20424770558151317	No Hit
GAATATGGCAAGAAAACTGAAAATC	1700	0.18323013165623872	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1389	0.14970979580618562	No Hit
GCCATATTCCACGTCCTACAGTGGA	1325	0.14281172026148017	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	1314	0.14162611352723392	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1291	0.1391471176283554	No Hit
GATATACACTGTTCTACAAATCCCG	1282	0.1381770757548812	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	1211	0.1305245231974736	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	1192	0.12847665702013913	No Hit
GAAATATGGCGAGGAAAACTGAAAA	1164	0.1254587489693305	No Hit
TTGTAGAACAGTGTATATCAATGAG	1134	0.12222527605774981	No Hit
CTGTAGGACATGGAATATGGCAAGA	1111	0.1197462801588713	No Hit
GACCTGGAATATGGCGAGAAAACTG	1071	0.11543498294343038	No Hit
GTCCACTGTAGGACGTGGAATATGG	1070	0.11532720051304438	No Hit
ACAGTGGACATTTCTAAATTTTCCA	1060	0.11424937620918413	No Hit
CCATATTTCACGTCCTAAAGTGTGT	1058	0.1140338113484121	No Hit
ACCTGGAATATGGCGAGAAAACTGA	1019	0.1098302965633572	No Hit
ATACACACTTTAGGACGTGAAATAT	970	0.10454895747444208	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	959	0.10336335074019586	No Hit
CCACTGTAGGACGTGGAATATGGCA	949	0.10228552643633561	No Hit
GTGTATATCAATGAGTTACAATGAG	943	0.10163883185401947	No Hit
GGTATCAACGCAGAGTACTTTTTTT	938	0.10109991970208936	No Hit
CACTTTAGGACGTGAAATATGGCGA	933	0.10056100755015925	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.0778243038602278E-4	0.0
12	0.0	0.0	0.0	1.0778243038602278E-4	0.0
13	0.0	0.0	0.0	1.0778243038602278E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACGT	45	3.5310695E-5	16.891861	19
GTCGAGA	50	8.694841E-5	15.202675	19
TCCAACG	170	0.0	15.090891	18
AATGTCC	805	0.0	14.396943	8
TGTCGAG	100	6.566552E-10	14.252508	18
TATTGCG	40	0.005272708	14.251739	16
CTACCGT	40	0.005285928	14.246362	4
TGTCCAC	820	0.0	13.787524	10
AAATGTC	820	0.0	13.786037	7
CCAACGA	140	0.0	13.573817	19
TAGGACC	965	0.0	13.286762	4
CTAGGAC	160	0.0	13.057756	3
GGCGAGG	1340	0.0	13.047071	19
ATGTCCA	880	0.0	12.955427	9
TAGAAAT	875	0.0	12.9167	4
GTCCTAT	185	0.0	12.840097	1
ATAGGAC	215	0.0	12.809301	3
AATGGCG	60	4.085053E-4	12.668213	16
TCTACAC	120	7.421477E-10	12.662067	3
ATGTCGA	115	5.296897E-9	12.393485	17
>>END_MODULE