FastQCFastQC Report
Thu 2 Feb 2017
SRR4064031_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064031_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927795
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA28220.30416201854935626No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA24510.26417473687614185No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA23670.2551210127237159No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC19740.21276251758200895No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA15010.1617814280094202No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA14560.15693121864204918No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA9800.10562678177830231No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA9290.10012987782861515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG357.2869134E-637.7156261
GACGTGT451.0370186E-634.2215927
TAGGACC11850.027.6619384
GGCGTGC508.340419E-526.3995158
CGTGTAA609.747098E-625.667589
TCGGAAA609.751177E-625.66619538
TTGCTCG450.001398118924.44399543
TCTCGCA450.001398118924.44399543
GTATCAA24650.024.3659781
AGGCGTG551.5934272E-423.999567
TTGCGAG651.8130597E-523.69187218
GGACCTG22350.022.836276
AGGACCT23850.022.1379595
TGCGTGT602.87139E-422.00078412
TCTTCGC500.002580265321.99959644
AAGGCGT500.002580265321.9995966
GACCTGG24650.021.240997
TCCGTAA755.4716507E-520.53295726
TTAGGAC21850.020.4389823
TGTAGGA38350.020.307322
Produced by FastQC (version 0.11.5)