##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064031_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	927795
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34572615717912	32.0	32.0	32.0	32.0	32.0
2	31.504736498903313	32.0	32.0	32.0	32.0	32.0
3	31.56299613599987	32.0	32.0	32.0	32.0	32.0
4	31.626336636864824	32.0	32.0	32.0	32.0	32.0
5	31.610371903276047	32.0	32.0	32.0	32.0	32.0
6	35.21505936117354	36.0	36.0	36.0	36.0	36.0
7	35.22591736321062	36.0	36.0	36.0	36.0	36.0
8	35.17886925452282	36.0	36.0	36.0	36.0	36.0
9	35.278585247818754	36.0	36.0	36.0	36.0	36.0
10	35.171686633361894	36.0	36.0	36.0	36.0	36.0
11	35.275414288716796	36.0	36.0	36.0	36.0	36.0
12	35.22353106020188	36.0	36.0	36.0	36.0	36.0
13	35.24281118134933	36.0	36.0	36.0	36.0	36.0
14	35.206248147489475	36.0	36.0	36.0	36.0	36.0
15	35.19002473606778	36.0	36.0	36.0	36.0	36.0
16	35.20252534234395	36.0	36.0	36.0	36.0	36.0
17	35.2092703668375	36.0	36.0	36.0	36.0	36.0
18	35.19770207858417	36.0	36.0	36.0	36.0	36.0
19	35.17115095468288	36.0	36.0	36.0	36.0	36.0
20	35.15768677347906	36.0	36.0	36.0	36.0	36.0
21	35.13691171002215	36.0	36.0	36.0	36.0	36.0
22	35.10978826141551	36.0	36.0	36.0	36.0	36.0
23	35.10307449382676	36.0	36.0	36.0	36.0	36.0
24	35.09135099887367	36.0	36.0	36.0	36.0	36.0
25	35.04679374215209	36.0	36.0	36.0	36.0	36.0
26	35.01631502648753	36.0	36.0	36.0	36.0	36.0
27	34.96003319698856	36.0	36.0	36.0	36.0	36.0
28	34.918778393934005	36.0	36.0	36.0	36.0	36.0
29	34.893743768828244	36.0	36.0	36.0	36.0	36.0
30	34.85266896243243	36.0	36.0	36.0	36.0	36.0
31	34.83590987233171	36.0	36.0	36.0	36.0	36.0
32	34.80470578091065	36.0	36.0	36.0	36.0	36.0
33	34.83416918608098	36.0	36.0	36.0	36.0	36.0
34	34.80946221956359	36.0	36.0	36.0	36.0	36.0
35	34.78798764813348	36.0	36.0	36.0	36.0	36.0
36	34.72473229538853	36.0	36.0	36.0	32.0	36.0
37	34.6400390172398	36.0	36.0	36.0	32.0	36.0
38	34.60593018931984	36.0	36.0	36.0	32.0	36.0
39	34.45494532736219	36.0	36.0	36.0	32.0	36.0
40	34.53468061371316	36.0	36.0	36.0	32.0	36.0
41	34.5152873210138	36.0	36.0	36.0	32.0	36.0
42	34.50131225108995	36.0	36.0	36.0	32.0	36.0
43	34.4949886558992	36.0	36.0	36.0	32.0	36.0
44	34.389863062422194	36.0	36.0	36.0	32.0	36.0
45	34.3606583350848	36.0	36.0	36.0	32.0	36.0
46	34.20385537753491	36.0	36.0	36.0	32.0	36.0
47	34.21662112858983	36.0	36.0	36.0	32.0	36.0
48	34.15471413404901	36.0	36.0	36.0	32.0	36.0
49	34.09623462079446	36.0	36.0	36.0	32.0	36.0
50	33.66561794361901	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	2.0
19	1.0
20	4.0
21	11.0
22	56.0
23	183.0
24	465.0
25	1233.0
26	2657.0
27	5533.0
28	9431.0
29	15048.0
30	21566.0
31	31044.0
32	44983.0
33	72106.0
34	148902.0
35	574567.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.96865423399593	21.938802458077195	13.01496038123695	25.077582926689928
2	10.934551133711938	22.63479030344053	47.30246940858575	19.12818915426178
3	17.13325831651922	29.244593615810437	31.21389276906893	22.40825529860141
4	9.84430827930739	18.90493050727801	42.047758394904044	29.203002818510555
5	9.345167844189719	42.35774066469425	37.30134350799476	10.995747983121271
6	27.585385517443566	41.59326659423664	19.945311017986793	10.876036870333007
7	24.97116816879609	33.648417644434005	24.536588941079593	16.843825245690315
8	21.836720396208214	43.37854806288027	21.140661460775277	13.644070080136236
9	26.775678874223154	14.85256298327416	21.935015186691757	36.43674295581093
10	13.752617788350127	31.329712531001192	36.536079940762264	18.381589739886415
11	32.2767769569538	22.291909626490362	29.063348113913218	16.367965302642613
12	24.19893230494302	25.427439094711858	35.214967131677	15.15866146866812
13	29.37260789852909	24.71833695662718	27.03383190000356	18.875223244840175
14	18.15476479179129	25.78522195096977	29.691365010589628	26.36864824664931
15	19.198213317553268	39.064384593744236	23.773353468002796	17.964048620699693
16	17.387827162915627	29.889942411350884	34.195348716892994	18.5268817088405
17	17.5794221783907	32.838396413000716	31.171541127080875	18.41064028152771
18	17.736734095573144	28.957891423939845	36.972834428406365	16.33254005208064
19	22.748045363518195	26.98756404977829	29.502777556224764	20.76161303047875
20	23.425045996449622	29.60727061864282	30.726598396833765	16.241084988073798
21	20.81569743316142	27.91748177129646	28.34710253881515	22.91971825672697
22	22.128056305541634	32.73266184879203	28.708173680608322	16.431108165058014
23	18.30933542001468	32.62692271626415	31.14641367811	17.91732818561117
24	22.096834517330986	28.35536959373306	32.25496314355958	17.292832745376373
25	19.821768667888936	30.96133470865493	31.591007811093757	17.625888812362373
26	16.55575173163113	32.51057949550412	32.80247224970843	18.131196523156323
27	18.193821962666185	31.87171378126152	31.268741821505643	18.665722434566657
28	17.26465011851164	32.49633255259729	29.526589397870346	20.71242793102073
29	19.69380474124086	29.880768376781415	29.130909036036794	21.29451784594093
30	17.692621104301356	30.22848729296775	31.212907732006546	20.865983870724353
31	21.530572868048264	28.534964288717045	30.63829741365372	19.296165429580974
32	18.65071580549406	31.93294429845457	30.631861913456625	18.78447798259475
33	17.22354980258754	32.55694134106815	32.868662240919726	17.35084661542458
34	19.008610979672742	31.70207239086454	32.44956449288562	16.8397521365771
35	17.91065046153581	32.571903441793886	31.7652397356247	17.7522063610456
36	16.984742081350415	31.279757253035683	33.29212096387361	18.443379701740298
37	17.684511420505928	31.13323366686032	29.34980021794406	21.832454694689687
38	18.813805413900486	33.63762674850144	29.256362082216352	18.292205755381723
39	21.328783261888155	29.30150439072553	30.374744947728434	18.994967399657884
40	22.190461408455562	29.08227056764191	31.419492804235542	17.307775219666986
41	21.63016297318272	28.059735276364577	32.457855479865486	17.852246270587223
42	19.374504190666713	32.3432595798986	31.376862518538957	16.90537371089573
43	17.096386516127293	32.86583492764949	32.145293848185176	17.892484708038047
44	16.707750444879654	32.40955011915553	31.058905191861424	19.82379424410339
45	17.258918264644795	32.769913784090534	30.675898670707824	19.29526928055685
46	18.489756236365363	29.37591077233494	31.110366205927242	21.02396678537246
47	21.367880651583203	29.59070718959743	30.243993399233215	18.797418759586147
48	17.54463116437138	30.82398566025452	30.37753928501753	21.253843890356563
49	20.456137703575163	29.533299561323144	30.02629905474299	19.9842636803587
50	18.034742888184464	33.52913977022743	29.05774709065766	19.37837025093044
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	6.5
2	7.0
3	37.5
4	68.0
5	92.0
6	116.0
7	128.5
8	141.0
9	204.0
10	267.0
11	420.5
12	574.0
13	1122.5
14	1671.0
15	2316.5
16	2962.0
17	3426.0
18	3890.0
19	4328.5
20	4767.0
21	5652.0
22	6537.0
23	8320.0
24	10103.0
25	12451.0
26	14799.0
27	20385.5
28	25972.0
29	33673.0
30	41374.0
31	51680.5
32	61987.0
33	76757.5
34	91528.0
35	106354.0
36	121180.0
37	119175.5
38	117171.0
39	106722.0
40	96273.0
41	86419.5
42	76566.0
43	68576.5
44	60587.0
45	54440.5
46	48294.0
47	43911.5
48	39529.0
49	34998.5
50	30468.0
51	26491.0
52	22514.0
53	19335.0
54	16156.0
55	13594.5
56	11033.0
57	9297.0
58	7561.0
59	6312.0
60	5063.0
61	4173.5
62	3284.0
63	2600.5
64	1917.0
65	1596.5
66	1276.0
67	1049.5
68	823.0
69	665.5
70	508.0
71	409.0
72	310.0
73	267.0
74	224.0
75	175.0
76	126.0
77	97.0
78	68.0
79	55.5
80	43.0
81	31.5
82	20.0
83	16.0
84	12.0
85	8.5
86	5.0
87	3.0
88	1.0
89	3.0
90	5.0
91	3.5
92	2.0
93	2.5
94	3.0
95	2.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008407029570109777
2	2.1556486077204553E-4
3	3.2334729115806834E-4
4	0.0
5	0.0
6	3.2334729115806834E-4
7	1.0778243038602277E-4
8	0.0
9	9.700418734742049E-4
10	8.622594430881821E-4
11	1.0778243038602277E-4
12	2.1556486077204553E-4
13	2.1556486077204553E-4
14	0.0
15	0.0045268620762129565
16	8.622594430881821E-4
17	0.0
18	3.2334729115806834E-4
19	1.0778243038602277E-4
20	6.466945823161367E-4
21	0.0
22	0.0
23	6.466945823161367E-4
24	6.466945823161367E-4
25	0.0012933891646322733
26	0.006143598532003298
27	0.006143598532003298
28	0.004095732354668866
29	0.0022634310381064783
30	0.004419079645826934
31	0.008299247139723754
32	0.0031256904811946605
33	0.0045268620762129565
34	0.002802343190036592
35	0.002478995898878524
36	0.008622594430881822
37	0.0036646026331247743
38	0.007760334987793639
39	0.004742426936985002
40	0.002910125620422615
41	0.003772385063510797
42	0.002047866177334433
43	0.0021556486077204556
44	0.0017245188861763642
45	8.622594430881821E-4
46	0.0011856067342462505
47	0.0025867783292645467
48	0.0025867783292645467
49	5.389121519301139E-4
50	8.622594430881821E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	927795.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.07557120102072
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.72661249105735	59.50916807528418
2	11.3515858143576	16.13640891585741
3	2.700482601402875	5.758150302393832
4	0.8878467889056415	2.524168706418421
5	0.38509503699707237	1.3685424860622564
6	0.21567891883357543	0.9197701411268975
7	0.13141287946183464	0.6538171829644547
8	0.09623693002213299	0.547207581756461
9	0.0678482498677834	0.4340117802908056
>10	0.3807978638219846	4.962303117119292
>50	0.03247309590196756	1.6408992719868172
>100	0.01996651091188254	2.7818219214429156
>500	0.003048321890982536	1.4019064214616543
>1k	9.144965672947607E-4	1.361824095834515
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2822	0.30416201854935626	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	2451	0.26417473687614185	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	2367	0.2551210127237159	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	1974	0.21276251758200895	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1501	0.1617814280094202	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	1456	0.15693121864204918	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	980	0.10562678177830231	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	929	0.10012987782861515	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	1.0778243038602278E-4	0.0
16	0.0	0.0	0.0	1.0778243038602278E-4	0.0
17	0.0	0.0	0.0	1.0778243038602278E-4	0.0
18	0.0	0.0	0.0	3.2334729115806834E-4	0.0
19	0.0	0.0	0.0	3.2334729115806834E-4	0.0
20	0.0	0.0	0.0	4.311297215440911E-4	0.0
21	0.0	0.0	0.0	5.389121519301139E-4	0.0
22	0.0	0.0	0.0	6.466945823161367E-4	0.0
23	0.0	0.0	0.0	9.70041873474205E-4	0.0
24	0.0	0.0	0.0	0.0018323013165623871	0.0
25	0.0	0.0	0.0	0.00194008374694841	0.0
26	0.0	0.0	0.0	0.0022634310381064783	0.0
27	0.0	0.0	0.0	0.002371213468492501	0.0
28	0.0	0.0	0.0	0.0042035147850548885	0.0
29	0.0	0.0	0.0	0.0076525525574076166	0.0
30	0.0	0.0	0.0	0.016382929418675463	0.0
31	0.0	0.0	0.0	0.040202846533986494	0.0
32	0.0	0.0	0.0	0.07221422835863527	0.0
33	0.0	0.0	0.0	0.1017466142844055	0.0
34	0.0	0.0	0.0	0.13214125965326393	0.0
35	0.0	0.0	0.0	0.16824837383258154	0.0
36	0.0	0.0	0.0	0.2200917228482585	0.0
37	0.0	0.0	0.0	0.30664101444823477	0.0
38	0.0	0.0	0.0	0.42487834058170176	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTAGG	35	7.2869134E-6	37.715626	1
GACGTGT	45	1.0370186E-6	34.221592	7
TAGGACC	1185	0.0	27.661938	4
GGCGTGC	50	8.340419E-5	26.399515	8
CGTGTAA	60	9.747098E-6	25.66758	9
TCGGAAA	60	9.751177E-6	25.666195	38
TTGCTCG	45	0.0013981189	24.443995	43
TCTCGCA	45	0.0013981189	24.443995	43
GTATCAA	2465	0.0	24.365978	1
AGGCGTG	55	1.5934272E-4	23.99956	7
TTGCGAG	65	1.8130597E-5	23.691872	18
GGACCTG	2235	0.0	22.83627	6
AGGACCT	2385	0.0	22.137959	5
TGCGTGT	60	2.87139E-4	22.000784	12
TCTTCGC	50	0.0025802653	21.999596	44
AAGGCGT	50	0.0025802653	21.999596	6
GACCTGG	2465	0.0	21.24099	7
TCCGTAA	75	5.4716507E-5	20.532957	26
TTAGGAC	2185	0.0	20.438982	3
TGTAGGA	3835	0.0	20.30732	2
>>END_MODULE