FastQCFastQC Report
Thu 2 Feb 2017
SRR4064030_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064030_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544188
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17110.3144134012510382No Hit
TATCAACGCAGAGTACTTTTTTTTT12400.22786242989555078No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11570.21261034789447764No Hit
GTATCTGATCGTCTTCGAACCTCCG8550.15711482061346446No Hit
GAGTACTTTTTTTTTTTTTTTTTTT7970.14645673921512417No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG7680.14112769851595405No Hit
GAACTACGACGGTATCTGATCGTCT7450.1369012179614398No Hit
CTATTGGAGCTGGAATTACCGCGGC7390.1357986578167839No Hit
GTCCTATTCCATTATTCCTAGCTGC7290.13396105757569077No Hit
GAATAGGACCGCGGTTCTATTTTGT7250.1332260174792535No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7090.13028585709350446No Hit
GCGCAAGACGGACCAGAGCGAAAGC6930.12734569670775542No Hit
GATTAAGAGGGACGGCCGGGGGCAT6930.12734569670775542No Hit
CTTTAATATACGCTATTGGAGCTGG6790.12477305637022498No Hit
GTCTTGCGCCGGTCCAAGAATTTCA6740.12385425624967843No Hit
ATATTAAAGTTGCTGCAGTTAAAAA6430.11815769550228965No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG6310.11595257521297786No Hit
ATACAGGACTCTTTCGAGGCCCTGT6210.1141149749718847No Hit
GAATAATGGAATAGGACCGCGGTTC5940.10915345432093321No Hit
GCTTTGAACACTCTAATTTTTTCAA5940.10915345432093321No Hit
GTACATGGGGAATAATTGCAATCCC5940.10915345432093321No Hit
GAATAACGCCGCCGCATCGCCAGTC5730.10529449381463758No Hit
CTCTTAATCATGGCCTCAGTTCCGA5690.10455945371820033No Hit
GGTATCAACGCAGAGTACTTTTTTT5670.1041919336699817No Hit
ATCAGATACCGTCGTAGTTCCGACC5480.1007004932119047No Hit
GTCCTGTATTGTTATTTTTCGTCAC5480.1007004932119047No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGT250.006044696618.9904382
ATAACGA753.259629E-916.4689812
TAAGACA755.8376827E-815.1965434
CGTTATT508.734774E-515.192352
CGTCTTA653.3574906E-614.61743715
ATAGGGT653.3767774E-614.6093723
GCGTTAT602.5699379E-514.2454481
GTACTGG400.005284451414.2454481
GTTATTC602.5721678E-514.2441383
ATAGATT551.9602446E-413.8150394
GTATTAG1450.013.7542261
TAACGAA903.6192432E-813.72288713
AACGAAC903.6192432E-813.72288714
TCTCGTC707.2312905E-613.57333412
CATAGGG852.7046372E-713.4050162
ACGCCCC655.4373068E-513.15448317
CGGTGAG801.9894505E-613.06433515
TAGGGTC801.9976887E-613.0595294
GTATTAA801.9997533E-613.0583281
CGAACGA957.338531E-813.00182516
Produced by FastQC (version 0.11.5)