##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064030_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544188
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97442979264519	32.0	32.0	32.0	32.0	32.0
2	30.38997736076503	32.0	32.0	32.0	32.0	32.0
3	30.407956441523886	32.0	32.0	32.0	21.0	32.0
4	30.388790271009285	32.0	32.0	32.0	21.0	32.0
5	30.329150955184605	32.0	32.0	32.0	21.0	32.0
6	33.690118120943495	36.0	36.0	36.0	21.0	36.0
7	33.67205083537307	36.0	36.0	36.0	21.0	36.0
8	33.5844138422751	36.0	36.0	36.0	21.0	36.0
9	33.84782832403508	36.0	36.0	36.0	32.0	36.0
10	33.30960623902034	36.0	36.0	36.0	21.0	36.0
11	33.86697244334678	36.0	36.0	36.0	32.0	36.0
12	33.53647820238594	36.0	36.0	36.0	21.0	36.0
13	33.74361250156196	36.0	36.0	36.0	32.0	36.0
14	33.51402272743978	36.0	36.0	36.0	21.0	36.0
15	33.402048556748774	36.0	36.0	36.0	21.0	36.0
16	33.4405609826016	36.0	36.0	36.0	21.0	36.0
17	33.31529912456725	36.0	36.0	36.0	21.0	36.0
18	33.34614691981447	36.0	36.0	36.0	21.0	36.0
19	33.322254441479785	36.0	36.0	36.0	21.0	36.0
20	33.30457856476071	36.0	36.0	36.0	21.0	36.0
21	33.25518754548061	36.0	36.0	36.0	21.0	36.0
22	33.22907524605467	36.0	36.0	36.0	21.0	36.0
23	33.19874749167567	36.0	36.0	36.0	21.0	36.0
24	33.15025873411395	36.0	36.0	36.0	21.0	36.0
25	32.33997074540416	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	7.0
5	6.0
6	20.0
7	8.0
8	40.0
9	38.0
10	44.0
11	21.0
12	29.0
13	23.0
14	130.0
15	311.0
16	479.0
17	542.0
18	746.0
19	927.0
20	1214.0
21	1504.0
22	2149.0
23	2859.0
24	3901.0
25	5395.0
26	7896.0
27	10875.0
28	14720.0
29	20367.0
30	27606.0
31	39533.0
32	57758.0
33	80053.0
34	137822.0
35	127164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.39081059817874	18.37347293655725	11.403482972989865	25.832233492274153
2	16.16994141472056	20.439977653795545	38.461015429172086	24.929065502311804
3	17.749792807907664	25.779290346410477	29.480081075965536	26.990835769716327
4	11.53493466232246	15.845328599544631	38.35471440148629	34.26502233664662
5	13.389152096327797	37.96545754600821	34.426124063204085	14.219266294459901
6	31.430876588111833	37.95736610055484	17.879111447637566	12.73264586369576
7	28.813727544414913	31.629337185750465	21.97605472784952	17.580880541985096
8	27.506332697306256	32.990687534237004	20.364814835239574	19.13816493321716
9	27.842399707316893	13.887004465623523	19.51545502846858	38.755140798591
10	15.10347718647957	26.59260730150325	32.775681758009	25.528233754008177
11	36.24524961252283	21.6985381584584	23.60494426395883	18.451267965059948
12	24.036141919919977	24.75269188902945	29.628982671883737	21.58218351916684
13	28.312062266825777	20.096423114334204	26.468365532597964	25.12314908624205
14	22.89415377260483	20.72059648251832	26.519384762482645	29.865864982394204
15	24.46665526707227	28.099080861735793	23.221341511622988	24.212922359568946
16	24.855614619284115	26.320889498526267	24.815714424931553	24.007781457258066
17	22.850912514111517	26.507588042994616	26.390650913249562	24.250848529644298
18	23.78431260769382	25.262097107874958	27.746893544715117	23.2066967397161
19	24.62536847118707	25.572406082372044	26.828380234681436	22.97384521175945
20	25.001103419865974	24.715317674579413	25.93091856025776	24.352660345296854
21	26.690045273831288	24.56390377380002	25.214324331282338	23.531726621086353
22	24.797204726219483	25.03627419428992	25.941611879913566	24.22490919957703
23	23.210692992103105	24.724785658325946	26.902408792146502	25.162112557424443
24	23.71585402855803	25.76548578994309	26.42987835713169	24.08878182436719
25	23.815355871362577	25.26903669049931	26.516756406861447	24.398851031276664
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	106.0
1	106.0
2	226.0
3	346.0
4	346.0
5	346.0
6	1030.0
7	1714.0
8	1714.0
9	1714.0
10	1722.5
11	1731.0
12	1731.0
13	1731.0
14	1972.5
15	2214.0
16	2214.0
17	2214.0
18	3695.5
19	5177.0
20	5177.0
21	5177.0
22	8253.0
23	11329.0
24	11329.0
25	11329.0
26	16325.0
27	21321.0
28	21321.0
29	21321.0
30	25024.0
31	28727.0
32	28727.0
33	28727.0
34	34763.5
35	40800.0
36	40800.0
37	40800.0
38	44931.5
39	49063.0
40	49063.0
41	49063.0
42	54389.5
43	59716.0
44	59716.0
45	59716.0
46	65653.0
47	71590.0
48	71590.0
49	71590.0
50	70977.5
51	70365.0
52	70365.0
53	70365.0
54	63056.5
55	55748.0
56	55748.0
57	55748.0
58	50702.5
59	45657.0
60	45657.0
61	45657.0
62	39847.0
63	34037.0
64	34037.0
65	34037.0
66	27830.5
67	21624.0
68	21624.0
69	21624.0
70	15754.5
71	9885.0
72	9885.0
73	9885.0
74	7756.5
75	5628.0
76	5628.0
77	5628.0
78	4992.5
79	4357.0
80	4357.0
81	4357.0
82	2922.0
83	1487.0
84	1487.0
85	1487.0
86	1188.0
87	889.0
88	889.0
89	889.0
90	615.5
91	342.0
92	342.0
93	342.0
94	219.0
95	96.0
96	96.0
97	96.0
98	167.5
99	239.0
100	239.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031239204098583575
2	0.004410240578623564
3	0.001286320168765206
4	0.0027564003616397273
5	0.008452961109028496
6	0.012311921615324115
7	0.020948642748461928
8	0.03417936448433262
9	0.04685880614787537
10	0.055128007232794554
11	0.052371606871154816
12	0.061008328004292636
13	0.06155960807662059
14	0.061192088028401946
15	0.056781847449778385
16	0.06082456798018332
17	0.05623056737745044
18	0.07368776966783538
19	0.07111512933030496
20	0.07791425022234963
21	0.07093136930619565
22	0.07589288995714716
23	0.07901681036700553
24	0.07038008923386771
25	0.0727689695472888
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	544188.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.475857337619104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.1164607237276	46.664327748549525
2	10.139817725800608	11.250301631719516
3	2.418175966758035	4.0245115484738365
4	0.9201400507542556	2.0418223274509075
5	0.4729684052713467	1.311916388801722
6	0.29271401667052976	0.9743136617721462
7	0.20340382532741708	0.789880111705285
8	0.14706437434866118	0.6526817800650031
9	0.12688765783713207	0.6335281443669629
>10	0.9026275862773764	10.3390887410855
>50	0.13485205258606836	5.377683610639138
>100	0.11525532521386639	12.080394518413458
>500	0.008635845693258001	3.102601895960187
>1k	9.964437338374616E-4	0.7569478909968196
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1711	0.3144134012510382	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1240	0.22786242989555078	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1157	0.21261034789447764	No Hit
GTATCTGATCGTCTTCGAACCTCCG	855	0.15711482061346446	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	797	0.14645673921512417	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	768	0.14112769851595405	No Hit
GAACTACGACGGTATCTGATCGTCT	745	0.1369012179614398	No Hit
CTATTGGAGCTGGAATTACCGCGGC	739	0.1357986578167839	No Hit
GTCCTATTCCATTATTCCTAGCTGC	729	0.13396105757569077	No Hit
GAATAGGACCGCGGTTCTATTTTGT	725	0.1332260174792535	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	709	0.13028585709350446	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	693	0.12734569670775542	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	693	0.12734569670775542	No Hit
CTTTAATATACGCTATTGGAGCTGG	679	0.12477305637022498	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	674	0.12385425624967843	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	643	0.11815769550228965	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	631	0.11595257521297786	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	621	0.1141149749718847	No Hit
GAATAATGGAATAGGACCGCGGTTC	594	0.10915345432093321	No Hit
GCTTTGAACACTCTAATTTTTTCAA	594	0.10915345432093321	No Hit
GTACATGGGGAATAATTGCAATCCC	594	0.10915345432093321	No Hit
GAATAACGCCGCCGCATCGCCAGTC	573	0.10529449381463758	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	569	0.10455945371820033	No Hit
GGTATCAACGCAGAGTACTTTTTTT	567	0.1041919336699817	No Hit
ATCAGATACCGTCGTAGTTCCGACC	548	0.1007004932119047	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	548	0.1007004932119047	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCCGT	25	0.0060446966	18.990438	2
ATAACGA	75	3.259629E-9	16.46898	12
TAAGACA	75	5.8376827E-8	15.196543	4
CGTTATT	50	8.734774E-5	15.19235	2
CGTCTTA	65	3.3574906E-6	14.617437	15
ATAGGGT	65	3.3767774E-6	14.609372	3
GCGTTAT	60	2.5699379E-5	14.245448	1
GTACTGG	40	0.0052844514	14.245448	1
GTTATTC	60	2.5721678E-5	14.244138	3
ATAGATT	55	1.9602446E-4	13.815039	4
GTATTAG	145	0.0	13.754226	1
TAACGAA	90	3.6192432E-8	13.722887	13
AACGAAC	90	3.6192432E-8	13.722887	14
TCTCGTC	70	7.2312905E-6	13.573334	12
CATAGGG	85	2.7046372E-7	13.405016	2
ACGCCCC	65	5.4373068E-5	13.154483	17
CGGTGAG	80	1.9894505E-6	13.064335	15
TAGGGTC	80	1.9976887E-6	13.059529	4
GTATTAA	80	1.9997533E-6	13.058328	1
CGAACGA	95	7.338531E-8	13.001825	16
>>END_MODULE