FastQCFastQC Report
Thu 2 Feb 2017
SRR4064030_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064030_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544188
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8020.14737553933567077No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7880.14480289899814036No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7760.1425977787088286No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC7550.13873881820253295No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT7020.12899953692473923No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA6970.12808073680419266No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC6860.12605937653899021No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG6830.12550809646666225No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA6400.1176064154299617No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6040.11099105456202636No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT6010.11043977448969841No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC6010.11043977448969841No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG6000.11025601446558911No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT5910.10860217424860526No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG5850.10749961410394936No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC5480.1007004932119047No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACT300.005734830629.34111832
CTCGCGT300.005739986429.33572439
GTATTAT454.0734252E-529.324941
CGGTTAT554.951329E-628.0022835
AGCGGTT655.912152E-727.08161733
CGTCTTA701.1313641E-625.14028415
CGTCGTA1900.024.31106810
GTATCAA11600.023.5105131
ACCGTCG2000.023.093398
GCGGTTA703.2091448E-522.00179134
CCGTCGT2100.021.9957289
CGCTTAG703.2159835E-521.99572618
CTATACA703.218266E-521.9937064
TATACAA500.002582919821.9937065
ATACCGT2100.021.9937066
TACCGTC2150.021.4822247
GTCGTAG2300.021.0393911
TCGCTCG1057.6974175E-820.9598727
CGGCGTT2350.020.59742241
ACTATAC654.9368886E-420.3018823
Produced by FastQC (version 0.11.5)