##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064030_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544188
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.973448514116445	32.0	32.0	32.0	32.0	32.0
2	30.734428175556978	32.0	32.0	32.0	32.0	32.0
3	31.13135166523334	32.0	32.0	32.0	32.0	32.0
4	31.32529383227855	32.0	32.0	32.0	32.0	32.0
5	31.188580049541702	32.0	32.0	32.0	32.0	32.0
6	34.75430549736488	36.0	36.0	36.0	32.0	36.0
7	34.78997515564474	36.0	36.0	36.0	32.0	36.0
8	34.691055297066455	36.0	36.0	36.0	32.0	36.0
9	34.927197953648374	36.0	36.0	36.0	32.0	36.0
10	34.636355450689834	36.0	36.0	36.0	32.0	36.0
11	34.93859291274339	36.0	36.0	36.0	36.0	36.0
12	34.75825817548347	36.0	36.0	36.0	32.0	36.0
13	34.862586091571295	36.0	36.0	36.0	32.0	36.0
14	34.785710085485164	36.0	36.0	36.0	32.0	36.0
15	34.714793416980896	36.0	36.0	36.0	32.0	36.0
16	34.75852095231795	36.0	36.0	36.0	32.0	36.0
17	34.69020632575507	36.0	36.0	36.0	32.0	36.0
18	34.70404529317074	36.0	36.0	36.0	32.0	36.0
19	34.708016347291746	36.0	36.0	36.0	32.0	36.0
20	34.700654920725924	36.0	36.0	36.0	32.0	36.0
21	34.69204392599616	36.0	36.0	36.0	32.0	36.0
22	34.67475945812844	36.0	36.0	36.0	32.0	36.0
23	34.62183657118496	36.0	36.0	36.0	32.0	36.0
24	34.58260380603762	36.0	36.0	36.0	32.0	36.0
25	34.552353230868746	36.0	36.0	36.0	32.0	36.0
26	34.461138430101364	36.0	36.0	36.0	32.0	36.0
27	34.48305916337736	36.0	36.0	36.0	32.0	36.0
28	34.45399567796423	36.0	36.0	36.0	32.0	36.0
29	34.396135159172935	36.0	36.0	36.0	32.0	36.0
30	34.38560203459099	36.0	36.0	36.0	32.0	36.0
31	34.38056517233015	36.0	36.0	36.0	32.0	36.0
32	34.32936044161209	36.0	36.0	36.0	32.0	36.0
33	34.294300866612275	36.0	36.0	36.0	32.0	36.0
34	34.290037634052936	36.0	36.0	36.0	32.0	36.0
35	34.23323924820099	36.0	36.0	36.0	32.0	36.0
36	34.18846979352724	36.0	36.0	36.0	32.0	36.0
37	34.17497629495689	36.0	36.0	36.0	32.0	36.0
38	34.12640117018383	36.0	36.0	36.0	32.0	36.0
39	34.14082265687593	36.0	36.0	36.0	32.0	36.0
40	34.107402221291174	36.0	36.0	36.0	32.0	36.0
41	34.05503245202026	36.0	36.0	36.0	32.0	36.0
42	33.985063985240394	36.0	36.0	36.0	32.0	36.0
43	34.001165038552855	36.0	36.0	36.0	32.0	36.0
44	33.91075143149059	36.0	36.0	36.0	32.0	36.0
45	33.86102045616588	36.0	36.0	36.0	32.0	36.0
46	33.8301836865201	36.0	36.0	36.0	32.0	36.0
47	33.81801877292406	36.0	36.0	36.0	32.0	36.0
48	33.760968635839085	36.0	36.0	36.0	32.0	36.0
49	33.71604666034532	36.0	36.0	36.0	27.0	36.0
50	32.98910303057032	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	5.0
21	19.0
22	51.0
23	166.0
24	378.0
25	994.0
26	2177.0
27	4013.0
28	7215.0
29	11699.0
30	17596.0
31	26605.0
32	41192.0
33	68925.0
34	130784.0
35	232367.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.13771291952198	19.663849485722057	12.164782114116962	26.033655480639002
2	15.470657956393504	21.7111827779156	37.529700377628934	25.288458888061964
3	17.210659590654668	26.57657492107478	29.853028192566512	26.359737295704043
4	11.413702617477783	16.795114923519076	38.688467955926996	33.10271450307614
5	13.465567046682397	38.350717031614074	33.542819760817956	14.640896160885578
6	31.64702574666429	38.670346774178725	17.167811799987874	12.514815679169109
7	28.752065007065585	31.978713199690546	21.743077287770564	17.5261445054733
8	27.45227752173881	33.51360191698457	19.960013818753815	19.074106742522805
9	27.78007479074212	13.728419563200015	19.043523801648337	39.44798184440953
10	15.05373182600003	26.83338723666995	32.71797774282228	25.39490319450774
11	36.71084257646255	21.537226105684066	23.42756547369659	18.324365844156798
12	23.944893942707193	24.81959326481522	29.555649069161888	21.679863723315698
13	28.49582403043083	19.989525620882603	26.288302691180387	25.226347657506174
14	22.933251008842532	20.691011194660668	26.313332892309276	30.062404904187524
15	24.651341432711515	28.153823253215528	23.01578404761861	24.17905126645435
16	24.91537757588454	26.488013201465687	24.470857176459347	24.125752046190428
17	22.90366564496093	26.549648283313854	26.183230795239883	24.363455276485332
18	24.075687078730144	25.323601402456504	27.5151234499842	23.085588068829153
19	24.958653994575407	25.60989952001882	26.538990201915514	22.892456283490265
20	25.130884279736783	24.939955860436655	25.66030177348429	24.26885808634228
21	26.79184399508993	24.708740361786734	24.995222239373156	23.504193403750172
22	24.96940384353879	25.073779920835893	25.80735263310706	24.149463602518257
23	23.243608779383443	24.67272099142937	26.89715243373565	25.18651779545154
24	23.98853331128201	25.9005669027996	26.06540055312072	24.04549923279767
25	23.976985387080653	25.2420172885243	26.26631824997795	24.5146790744171
26	23.912767822521808	25.875987996111416	26.872575378061846	23.33866880330493
27	24.67490223176218	25.556837895851096	25.593225322708697	24.175034549678028
28	23.31347419721188	25.447749399999264	26.9537236799065	24.285052722882355
29	23.80303949133543	25.525111637907273	26.67806016502196	23.993788705735337
30	23.681981149238567	25.61538786663702	26.84294768720747	23.859683296916952
31	24.44593094627276	25.477069075779742	25.63106903167474	24.44593094627276
32	23.897854698750017	25.734977929528856	25.37828530264662	24.98888206907451
33	23.64187014953625	25.232243374516905	26.359691922616786	24.766194553330063
34	24.281933954462758	25.341713067607547	26.433283716485654	23.943069261444034
35	25.514042564139316	25.233981479950092	25.965374600076814	23.28660135583378
36	23.85473351086411	25.75298962933558	25.84910618950216	24.543170670298146
37	25.057105841059236	25.639465970799296	25.314013212904175	23.989414975237292
38	23.80694222895242	25.384821776428474	26.43104449524762	24.377191499371495
39	24.69517405630298	25.396618292680685	25.287827360551894	24.62038029046444
40	25.06395036863345	25.624802452165856	26.023573428989366	23.28767375021133
41	23.86327092035408	25.92181501770583	26.399238474670643	23.815675587269443
42	25.405934844609096	26.381905853988414	25.343455058657494	22.868704242744993
43	24.25112232878399	25.2020926860732	26.05493392823344	24.491851056909375
44	24.065243237978677	25.897544741711826	25.45008168268162	24.587130337627876
45	24.364638041229156	26.34928277144611	25.37772567064453	23.908353516680204
46	23.908177778757842	26.007659202252185	25.440018229195378	24.64414478979459
47	24.30238690720115	25.743645909327295	25.831117448918274	24.122849734553277
48	25.49482980721346	26.374506824066437	24.59420372415593	23.536459644564175
49	24.12400952618967	26.589168369522078	25.076444731928905	24.21037737235935
50	24.363167934021586	26.52036649772685	24.913171793053007	24.203293775198556
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	36.5
2	19.0
3	55.5
4	92.0
5	107.5
6	123.0
7	129.5
8	136.0
9	180.0
10	224.0
11	304.5
12	385.0
13	728.0
14	1071.0
15	1380.0
16	1689.0
17	1872.5
18	2056.0
19	2081.0
20	2106.0
21	2531.0
22	2956.0
23	3333.0
24	3710.0
25	4579.0
26	5448.0
27	6623.5
28	7799.0
29	8758.0
30	9717.0
31	10399.0
32	11081.0
33	12639.0
34	14197.0
35	16106.0
36	18015.0
37	21317.0
38	24619.0
39	26079.0
40	27539.0
41	29743.5
42	31948.0
43	31458.0
44	30968.0
45	33641.5
46	36315.0
47	38338.5
48	40362.0
49	41052.5
50	41743.0
51	39268.5
52	36794.0
53	35973.0
54	35152.0
55	35163.0
56	35174.0
57	34402.0
58	33630.0
59	29462.5
60	25295.0
61	21899.5
62	18504.0
63	15966.0
64	13428.0
65	11290.0
66	9152.0
67	7788.0
68	6424.0
69	6311.0
70	6198.0
71	4762.5
72	3327.0
73	2897.5
74	2468.0
75	1872.0
76	1276.0
77	1214.0
78	1152.0
79	941.5
80	731.0
81	590.0
82	449.0
83	388.5
84	328.0
85	239.5
86	151.0
87	107.0
88	63.0
89	54.5
90	46.0
91	38.0
92	30.0
93	26.0
94	22.0
95	15.0
96	8.0
97	7.5
98	7.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.004961520650951509
2	5.512800723279455E-4
3	3.6752004821863035E-4
4	0.0
5	0.0
6	1.8376002410931517E-4
7	1.8376002410931517E-4
8	0.0
9	5.512800723279455E-4
10	7.350400964372607E-4
11	0.0
12	1.8376002410931517E-4
13	5.512800723279455E-4
14	0.0
15	0.0056965607473887705
16	0.001102560144655891
17	0.0
18	0.0
19	0.0
20	9.188001205465758E-4
21	0.0
22	3.6752004821863035E-4
23	7.350400964372607E-4
24	5.512800723279455E-4
25	0.002205120289311782
26	0.0056965607473887705
27	0.008085441060809868
28	0.004042720530404934
29	0.0033076804339676733
30	0.003491440458076988
31	0.0058803207714980855
32	0.004042720530404934
33	0.007166640940263292
34	0.0033076804339676733
35	0.0038589605062956185
36	0.010474321374230964
37	0.004226480554514248
38	0.007350400964372606
39	0.004594000602732879
40	0.0029401603857490428
41	0.003123920409858358
42	0.002205120289311782
43	0.001286320168765206
44	0.0016538402169838367
45	0.0018376002410931515
46	0.001102560144655891
47	0.002021360265202467
48	0.002021360265202467
49	7.350400964372607E-4
50	0.0018376002410931515
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	544188.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.106441425034994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.72507758013118	52.38354431806867
2	9.400660638061833	11.48881837272715
3	2.11661454026394	3.8801634727204743
4	0.7838618434088572	1.9159603127833313
5	0.39523333684146833	1.2075651373462155
6	0.25282621990320936	0.9269586358337089
7	0.17872427455131956	0.7644843089871458
8	0.12377211632853162	0.6050618861185628
9	0.10095278999926378	0.5551979173905484
>10	0.7206876055409115	9.031486544062412
>50	0.10668806184298717	4.634263215125735
>100	0.08885634388282701	10.249934387275152
>500	0.0060446492437297135	2.3565614915609148
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	802	0.14737553933567077	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	788	0.14480289899814036	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	776	0.1425977787088286	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	755	0.13873881820253295	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	702	0.12899953692473923	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	697	0.12808073680419266	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	686	0.12605937653899021	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	683	0.12550809646666225	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	640	0.1176064154299617	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	604	0.11099105456202636	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	601	0.11043977448969841	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	601	0.11043977448969841	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	600	0.11025601446558911	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	591	0.10860217424860526	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	585	0.10749961410394936	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC	548	0.1007004932119047	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	1.8376002410931517E-4	0.0
26	0.0	0.0	0.0	5.512800723279455E-4	0.0
27	0.0	0.0	0.0	5.512800723279455E-4	0.0
28	0.0	0.0	0.0	0.001102560144655891	0.0
29	0.0	0.0	0.0	0.0016538402169838365	0.0
30	0.0	0.0	0.0	0.003123920409858358	0.0
31	0.0	0.0	0.0	0.0055128007232794545	0.0
32	0.0	0.0	0.0	0.009555521253684389	0.0
33	0.0	0.0	0.0	0.012128161591214801	0.0
34	0.0	0.0	0.0	0.018008482362712887	0.0
35	0.0	0.0	0.0	0.024072563158320286	0.0
36	0.0	0.0	0.0	0.03307680433967673	0.0
37	0.0	0.0	0.0	0.049247686461296465	0.0
38	0.0	0.0	0.0	0.07111512933030496	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCACT	30	0.0057348306	29.341118	32
CTCGCGT	30	0.0057399864	29.335724	39
GTATTAT	45	4.0734252E-5	29.32494	1
CGGTTAT	55	4.951329E-6	28.00228	35
AGCGGTT	65	5.912152E-7	27.081617	33
CGTCTTA	70	1.1313641E-6	25.140284	15
CGTCGTA	190	0.0	24.311068	10
GTATCAA	1160	0.0	23.510513	1
ACCGTCG	200	0.0	23.09339	8
GCGGTTA	70	3.2091448E-5	22.001791	34
CCGTCGT	210	0.0	21.995728	9
CGCTTAG	70	3.2159835E-5	21.995726	18
CTATACA	70	3.218266E-5	21.993706	4
TATACAA	50	0.0025829198	21.993706	5
ATACCGT	210	0.0	21.993706	6
TACCGTC	215	0.0	21.482224	7
GTCGTAG	230	0.0	21.03939	11
TCGCTCG	105	7.6974175E-8	20.95987	27
CGGCGTT	235	0.0	20.597422	41
ACTATAC	65	4.9368886E-4	20.301882	3
>>END_MODULE