FastQCFastQC Report
Thu 2 Feb 2017
SRR4064029_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064029_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322490
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45641.4152376817885826No Hit
GTACTTTTTTTTTTTTTTTTTTTTT41861.298024744953332No Hit
TATCAACGCAGAGTACTTTTTTTTT35031.08623523210022No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25960.8049862011225154No Hit
GTACATGGGGTGGTATCAACGCAAA23690.734596421594468No Hit
GTACATGGAAGCAGTGGTATCAACG17900.5550559707277745No Hit
GTCCTAAAGTGTGTATTTCTCATTT17750.5504046637105026No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17750.5504046637105026No Hit
GGTATCAACGCAGAGTACTTTTTTT15890.4927284566963317No Hit
TCCATGTACTCTGCGTTGATACCAC15470.4797047970479705No Hit
GTCCTACAGTGGACATTTCTAAATT15410.47784427424106174No Hit
CTGTAGGACGTGGAATATGGCAAGA14760.4576886104995504No Hit
CTTTAGGACGTGAAATATGGCGAGG14720.4564482619616112No Hit
GTCCTACAGTGTGCATTTCTCATTT14140.43846320816149337No Hit
GTACATGGGTGGTATCAACGCAAAA12270.38047691401283756No Hit
GTATCAACGCAGAGTACATGGGGTG12240.37954665260938325No Hit
GTACATGGGAGTGGTATCAACGCAA10940.3392353251263605No Hit
CTGTAGGACCTGGAATATGGCGAGA9370.29055164501224845No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9020.2796985953052808No Hit
GTACATGGGAAGCAGTGGTATCAAC9020.2796985953052808No Hit
TATCAACGCAGAGTACATGGGGTGG8880.2753573754224937No Hit
CCCATGTACTCTGCGTTGATACCAC8690.2694657198672827No Hit
GTATTAGAGGCACTGCCTGCCCAGT7770.240937703494682No Hit
CATGTACTCTGCGTTGATACCACTG7600.23566622220844058No Hit
ATTTAGAAATGTCCACTGTAGGACG7250.22481317250147292No Hit
GTATCAACGCAGAGTACATGGGAAG7170.2223324754255946No Hit
CCATAGGGTCTTCTCGTCTTATTAT7000.21706099413935317No Hit
GAGTACATGGAAGCAGTGGTATCAA6690.20744829297032466No Hit
GGTATCAACGCAGAGTACATGGGGT6310.1956649818599026No Hit
GTATCAACGCAGAGTACATGGGAGT6160.19101367484263077No Hit
CTGAAGGACCTGGAATATGGCGAGA6010.18636236782535892No Hit
GTCCTTCAGTGTGCATTTCTCATTT5980.18543210642190455No Hit
GATATACACTGTTCTACAAATCCCG5670.17581940525287607No Hit
ATCAACGCAGAGTACTTTTTTTTTT5630.1745790567149369No Hit
TTTCTAAATTTTCCACCTTTTTCAG5590.17333870817699773No Hit
TATCAACGCAGAGTACATGGGAAGC5070.15721417718378863No Hit
CTCTAATACTTGTAATGCTAGAGGT5040.15628391578033426No Hit
TTGTAGAACAGTGTATATCAATGAG4960.15380321870445596No Hit
ACGCAGAGTACATGGGGTGGTATCA4940.1531830444354864No Hit
CCATTGGGATGTCCTGATCCAACAT4750.14729138888027535No Hit
GAATATGGCAAGAAAACTGAAAATC4610.1429501689974883No Hit
GCGTTGATACCACTGCTTCCATGTA4470.13860894911470123No Hit
GCTTCCATGTACTCTGCGTTGATAC4470.13860894911470123No Hit
CCTCTAGCATTACAAGTATTAGAGG4350.13488790350088375No Hit
CTTCTACACCATTGGGATGTCCTGA4330.13426772923191418No Hit
GATATATTTTGATCAACGGACCAAG4300.1333374678284598No Hit
GTATCAACGCAGAGTACATGGGTGG4130.12806598654221835No Hit
TATCAACGCAGAGTACATGGGAGTG4070.1262054637353096No Hit
CATGGAAGCAGTGGTATCAACGCAG4060.12589537660082484No Hit
GTGTATATCAATGAGTTACAATGAA4060.12589537660082484No Hit
GGTATCAACGCAGAGTACATGGGAA4020.12465502806288567No Hit
GTACATGGGAGAAATCGTAAATAGA4020.12465502806288567No Hit
GTTATATAATTTAAGCTCCATAGGG3880.1203138081800986No Hit
CTGTAGGACATGGAATATGGCAAGA3860.11969363391112903No Hit
ATCTATAACTTTATAGATGCAACAC3830.11876337250767464No Hit
GTCAGGATACCGCGGCCGTTAAACT3820.11845328537318986No Hit
GGAATATGGCGAGAAAACTGAAAAT3800.11783311110422029No Hit
ACCTATAACTTCTCTGTTAACCCAA3690.1144221526248876No Hit
GTGTATATCAATGAGTTACAATGAG3680.1141120654904028No Hit
GAGTACATGGGGTGGTATCAACGCA3600.11163136841452449No Hit
GGTATCAACGCAGAGTACATGGGAG3580.1110111941455549No Hit
TGCTAGAGGTGATGTTTTTGGTAAA3570.11070110701107011No Hit
GCCTAAAGGAAAGATCCAAAAAGAT3550.11008093274210054No Hit
CCTATAACTTCTCTGTTAACCCAAC3510.10884058420416137No Hit
CTGTTAGTATGAGTAACAAGAATTC3490.1082204099351918No Hit
GTATCAACGCAGAGTACATGGGAGA3490.1082204099351918No Hit
ATCCTGACCGTGCAAAGGTAGCATA3440.10666997426276785No Hit
GTTATAGATTAACCCAATTTTAAGT3380.10480945145585908No Hit
ATCGTAAATAGATAGAAACCGACCT3370.1044993643213743No Hit
GATTAAAGATAAGAGACAGTTGGAC3330.10325901578343513No Hit
GCGTTGATACCACTGCTTCCCATGT3290.10201866724549598No Hit
GTTCTACAGTGTGGTTTTTATCATT3270.10139849297652641No Hit
GTTCTACAAATCCCGTTTCCAACGA3230.10015814443858724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTTG250.00602193319.011
TCCAACG607.6292054E-817.41666818
GTCCTAT453.5248588E-516.888891
TTGTAGA855.638867E-1116.7647061
TATACTA508.6683365E-515.202365
CAAAAGG508.679825E-515.24
TTATACT508.679825E-515.24
GGACTTG456.7338394E-414.7800716
TGATTAA602.5520785E-514.25221210
CCAACGA602.5558184E-514.25000119
CCATTAA602.5558184E-514.2500011
CCACACT400.005267095314.254
AACCACA400.005272522614.2477882
CGAGGGT400.005272522614.24778817
TCGTCTT801.2800592E-714.24778814
CCTCTAG954.8457878E-914.01
CACCTTT1500.013.93117214
CCACCTT1500.013.93117213
CGCGTAT551.947436E-413.8224737
TAGGTGG551.9500032E-413.8203265
Produced by FastQC (version 0.11.5)