##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064029_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	322490
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98144438587243	32.0	32.0	32.0	32.0	32.0
2	30.875428695463427	32.0	32.0	32.0	32.0	32.0
3	30.92519457967689	32.0	32.0	32.0	32.0	32.0
4	30.860519706037397	32.0	32.0	32.0	32.0	32.0
5	30.94767899779838	32.0	32.0	32.0	32.0	32.0
6	34.50673199168966	36.0	36.0	36.0	32.0	36.0
7	34.32044404477658	36.0	36.0	36.0	32.0	36.0
8	34.20481875406989	36.0	36.0	36.0	32.0	36.0
9	34.2144159508822	36.0	36.0	36.0	32.0	36.0
10	34.162718223820896	36.0	36.0	36.0	32.0	36.0
11	34.423045675834906	36.0	36.0	36.0	32.0	36.0
12	34.27000837235263	36.0	36.0	36.0	32.0	36.0
13	34.29755651338026	36.0	36.0	36.0	32.0	36.0
14	34.2551614003535	36.0	36.0	36.0	32.0	36.0
15	34.27832801017086	36.0	36.0	36.0	32.0	36.0
16	34.26575707773885	36.0	36.0	36.0	32.0	36.0
17	34.21274768209867	36.0	36.0	36.0	32.0	36.0
18	34.21911687184099	36.0	36.0	36.0	32.0	36.0
19	34.13245682036652	36.0	36.0	36.0	32.0	36.0
20	34.04019349437192	36.0	36.0	36.0	32.0	36.0
21	33.95691339266334	36.0	36.0	36.0	32.0	36.0
22	33.96256318025365	36.0	36.0	36.0	32.0	36.0
23	34.01640981115693	36.0	36.0	36.0	32.0	36.0
24	33.99628825700022	36.0	36.0	36.0	32.0	36.0
25	33.66603305528854	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	3.0
5	7.0
6	15.0
7	4.0
8	14.0
9	25.0
10	23.0
11	9.0
12	35.0
13	24.0
14	71.0
15	101.0
16	152.0
17	197.0
18	219.0
19	281.0
20	452.0
21	594.0
22	948.0
23	1422.0
24	1960.0
25	2688.0
26	3580.0
27	4831.0
28	6506.0
29	8680.0
30	10987.0
31	15105.0
32	20861.0
33	29765.0
34	64977.0
35	147952.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.5656219757314	20.05415767140624	12.778232710489092	22.60198764237326
2	11.594508517256244	22.218983263196087	46.43447465290592	19.752033566641757
3	18.198985999348807	30.44389661534645	30.532893002775324	20.82422438252942
4	9.482817432507023	17.75252885468156	40.334534448861625	32.43011926394979
5	8.88498824638553	43.0774622116643	36.02684413900897	12.010705402941195
6	25.96644988416557	40.7913956885403	20.76149893159411	12.480655495700015
7	24.83017884726332	34.54643018877288	23.068071141880534	17.555319822083263
8	24.93524621946491	38.20922838309422	20.87630864676231	15.979216750678557
9	28.203107547965946	14.491548561642137	22.289582764526298	35.015761125865616
10	15.622343165302437	28.712431969517375	35.24646117375466	20.41876369142552
11	31.13201692874784	22.90035123428444	28.7977982698919	17.16983356707582
12	25.276552963189364	26.733091094141265	33.44204101517046	14.548314927498907
13	29.72721376994852	23.51435300542718	28.386089795107782	18.37234342951652
14	20.0822342901474	25.533281613653998	30.1377812257564	24.246702870442203
15	20.700879046546337	35.011372063509796	27.948901734821476	16.338847155122394
16	17.74549900391607	28.523996003252012	34.535253923825955	19.195251069005963
17	16.40084401278431	30.32860644800943	32.904707233065444	20.365842306140813
18	16.947369401074397	26.748224078056776	39.4973109019418	16.80709561892703
19	21.753513761781072	26.956891441906944	31.752179323657103	19.53741547265488
20	21.93502136679194	27.691796827721898	34.0251808496442	16.34800095584196
21	21.713750159048377	25.741630951900667	29.97092130131056	22.573697587740398
22	21.27750036465429	30.886373722546185	30.042858517238063	17.79326739556146
23	18.279806832853314	31.248137848842983	32.85858824113616	17.613467077167545
24	21.231437660020173	29.706261152921094	32.8525098921561	16.20979129490263
25	19.806715308532397	30.009713890242352	33.52223177404188	16.661339027183374
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	214.0
1	214.0
2	542.5
3	871.0
4	871.0
5	871.0
6	2990.5
7	5110.0
8	5110.0
9	5110.0
10	4455.0
11	3800.0
12	3800.0
13	3800.0
14	2892.0
15	1984.0
16	1984.0
17	1984.0
18	3479.0
19	4974.0
20	4974.0
21	4974.0
22	8242.5
23	11511.0
24	11511.0
25	11511.0
26	18721.5
27	25932.0
28	25932.0
29	25932.0
30	32328.0
31	38724.0
32	38724.0
33	38724.0
34	39022.5
35	39321.0
36	39321.0
37	39321.0
38	37576.5
39	35832.0
40	35832.0
41	35832.0
42	35296.0
43	34760.0
44	34760.0
45	34760.0
46	43062.0
47	51364.0
48	51364.0
49	51364.0
50	43221.0
51	35078.0
52	35078.0
53	35078.0
54	24870.0
55	14662.0
56	14662.0
57	14662.0
58	11427.5
59	8193.0
60	8193.0
61	8193.0
62	6392.0
63	4591.0
64	4591.0
65	4591.0
66	3720.5
67	2850.0
68	2850.0
69	2850.0
70	2129.5
71	1409.0
72	1409.0
73	1409.0
74	1023.5
75	638.0
76	638.0
77	638.0
78	477.0
79	316.0
80	316.0
81	316.0
82	211.5
83	107.0
84	107.0
85	107.0
86	71.5
87	36.0
88	36.0
89	36.0
90	29.5
91	23.0
92	23.0
93	23.0
94	19.0
95	15.0
96	15.0
97	15.0
98	95.0
99	175.0
100	175.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03038853917950944
2	0.008372352631089335
3	0.0015504356724239513
4	0.003721045613817483
5	0.011163136841452448
6	0.01457409532078514
7	0.0285280163726007
8	0.035660020465750876
9	0.05519550993829266
10	0.06449812397283637
11	0.061397252627988466
12	0.06790908245216906
13	0.06914943099010822
14	0.07442091227634966
15	0.06480821110732116
16	0.07069986666253217
17	0.06821916958665385
18	0.08155291636949982
19	0.07969239356259109
20	0.08093274210053024
21	0.08093274210053024
22	0.08279326490743899
23	0.08744457192471085
24	0.0821730906384694
25	0.08403361344537816
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	322490.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.433501814009738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.7952519231749	21.545474278272195
2	14.487747720209892	8.818257930478463
3	5.310509959753426	4.848522434804179
4	2.5238168016709968	3.0723433284753012
5	1.4183096438942382	2.15820645601414
6	0.9475775638086504	1.7302862104251293
7	0.6571908910285802	1.4000434121988279
8	0.47073208008558765	1.1460820490557846
9	0.3555963115798054	0.9739836894167261
>10	2.370981710734118	14.369747899159663
>50	0.3403128024861175	7.301311668578871
>100	0.28427326914259515	17.399919377345032
>500	0.020378012124917213	4.338739185711185
>1k	0.017321310306179633	10.897082080064498
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4564	1.4152376817885826	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4186	1.298024744953332	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3503	1.08623523210022	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2596	0.8049862011225154	No Hit
GTACATGGGGTGGTATCAACGCAAA	2369	0.734596421594468	No Hit
GTACATGGAAGCAGTGGTATCAACG	1790	0.5550559707277745	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1775	0.5504046637105026	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1775	0.5504046637105026	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1589	0.4927284566963317	No Hit
TCCATGTACTCTGCGTTGATACCAC	1547	0.4797047970479705	No Hit
GTCCTACAGTGGACATTTCTAAATT	1541	0.47784427424106174	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1476	0.4576886104995504	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1472	0.4564482619616112	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1414	0.43846320816149337	No Hit
GTACATGGGTGGTATCAACGCAAAA	1227	0.38047691401283756	No Hit
GTATCAACGCAGAGTACATGGGGTG	1224	0.37954665260938325	No Hit
GTACATGGGAGTGGTATCAACGCAA	1094	0.3392353251263605	No Hit
CTGTAGGACCTGGAATATGGCGAGA	937	0.29055164501224845	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	902	0.2796985953052808	No Hit
GTACATGGGAAGCAGTGGTATCAAC	902	0.2796985953052808	No Hit
TATCAACGCAGAGTACATGGGGTGG	888	0.2753573754224937	No Hit
CCCATGTACTCTGCGTTGATACCAC	869	0.2694657198672827	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	777	0.240937703494682	No Hit
CATGTACTCTGCGTTGATACCACTG	760	0.23566622220844058	No Hit
ATTTAGAAATGTCCACTGTAGGACG	725	0.22481317250147292	No Hit
GTATCAACGCAGAGTACATGGGAAG	717	0.2223324754255946	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	700	0.21706099413935317	No Hit
GAGTACATGGAAGCAGTGGTATCAA	669	0.20744829297032466	No Hit
GGTATCAACGCAGAGTACATGGGGT	631	0.1956649818599026	No Hit
GTATCAACGCAGAGTACATGGGAGT	616	0.19101367484263077	No Hit
CTGAAGGACCTGGAATATGGCGAGA	601	0.18636236782535892	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	598	0.18543210642190455	No Hit
GATATACACTGTTCTACAAATCCCG	567	0.17581940525287607	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	563	0.1745790567149369	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	559	0.17333870817699773	No Hit
TATCAACGCAGAGTACATGGGAAGC	507	0.15721417718378863	No Hit
CTCTAATACTTGTAATGCTAGAGGT	504	0.15628391578033426	No Hit
TTGTAGAACAGTGTATATCAATGAG	496	0.15380321870445596	No Hit
ACGCAGAGTACATGGGGTGGTATCA	494	0.1531830444354864	No Hit
CCATTGGGATGTCCTGATCCAACAT	475	0.14729138888027535	No Hit
GAATATGGCAAGAAAACTGAAAATC	461	0.1429501689974883	No Hit
GCGTTGATACCACTGCTTCCATGTA	447	0.13860894911470123	No Hit
GCTTCCATGTACTCTGCGTTGATAC	447	0.13860894911470123	No Hit
CCTCTAGCATTACAAGTATTAGAGG	435	0.13488790350088375	No Hit
CTTCTACACCATTGGGATGTCCTGA	433	0.13426772923191418	No Hit
GATATATTTTGATCAACGGACCAAG	430	0.1333374678284598	No Hit
GTATCAACGCAGAGTACATGGGTGG	413	0.12806598654221835	No Hit
TATCAACGCAGAGTACATGGGAGTG	407	0.1262054637353096	No Hit
CATGGAAGCAGTGGTATCAACGCAG	406	0.12589537660082484	No Hit
GTGTATATCAATGAGTTACAATGAA	406	0.12589537660082484	No Hit
GGTATCAACGCAGAGTACATGGGAA	402	0.12465502806288567	No Hit
GTACATGGGAGAAATCGTAAATAGA	402	0.12465502806288567	No Hit
GTTATATAATTTAAGCTCCATAGGG	388	0.1203138081800986	No Hit
CTGTAGGACATGGAATATGGCAAGA	386	0.11969363391112903	No Hit
ATCTATAACTTTATAGATGCAACAC	383	0.11876337250767464	No Hit
GTCAGGATACCGCGGCCGTTAAACT	382	0.11845328537318986	No Hit
GGAATATGGCGAGAAAACTGAAAAT	380	0.11783311110422029	No Hit
ACCTATAACTTCTCTGTTAACCCAA	369	0.1144221526248876	No Hit
GTGTATATCAATGAGTTACAATGAG	368	0.1141120654904028	No Hit
GAGTACATGGGGTGGTATCAACGCA	360	0.11163136841452449	No Hit
GGTATCAACGCAGAGTACATGGGAG	358	0.1110111941455549	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	357	0.11070110701107011	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	355	0.11008093274210054	No Hit
CCTATAACTTCTCTGTTAACCCAAC	351	0.10884058420416137	No Hit
CTGTTAGTATGAGTAACAAGAATTC	349	0.1082204099351918	No Hit
GTATCAACGCAGAGTACATGGGAGA	349	0.1082204099351918	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	344	0.10666997426276785	No Hit
GTTATAGATTAACCCAATTTTAAGT	338	0.10480945145585908	No Hit
ATCGTAAATAGATAGAAACCGACCT	337	0.1044993643213743	No Hit
GATTAAAGATAAGAGACAGTTGGAC	333	0.10325901578343513	No Hit
GCGTTGATACCACTGCTTCCCATGT	329	0.10201866724549598	No Hit
GTTCTACAGTGTGGTTTTTATCATT	327	0.10139849297652641	No Hit
GTTCTACAAATCCCGTTTCCAACGA	323	0.10015814443858724	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.1008713448479023E-4	0.0
10	0.0	0.0	0.0	3.1008713448479023E-4	0.0
11	0.0	0.0	0.0	3.1008713448479023E-4	0.0
12	0.0	0.0	0.0	3.1008713448479023E-4	0.0
13	0.0	0.0	0.0	3.1008713448479023E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAATTTG	25	0.006021933	19.0	11
TCCAACG	60	7.6292054E-8	17.416668	18
GTCCTAT	45	3.5248588E-5	16.88889	1
TTGTAGA	85	5.638867E-11	16.764706	1
TATACTA	50	8.6683365E-5	15.20236	5
CAAAAGG	50	8.679825E-5	15.2	4
TTATACT	50	8.679825E-5	15.2	4
GGACTTG	45	6.7338394E-4	14.780071	6
TGATTAA	60	2.5520785E-5	14.252212	10
CCAACGA	60	2.5558184E-5	14.250001	19
CCATTAA	60	2.5558184E-5	14.250001	1
CCACACT	40	0.0052670953	14.25	4
AACCACA	40	0.0052725226	14.247788	2
CGAGGGT	40	0.0052725226	14.247788	17
TCGTCTT	80	1.2800592E-7	14.247788	14
CCTCTAG	95	4.8457878E-9	14.0	1
CACCTTT	150	0.0	13.931172	14
CCACCTT	150	0.0	13.931172	13
CGCGTAT	55	1.947436E-4	13.822473	7
TAGGTGG	55	1.9500032E-4	13.820326	5
>>END_MODULE