##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064029_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	322490
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.80815529163695	32.0	32.0	32.0	32.0	32.0
2	31.420865763279483	32.0	32.0	32.0	32.0	32.0
3	31.4904586188719	32.0	32.0	32.0	32.0	32.0
4	31.57845824676734	32.0	32.0	32.0	32.0	32.0
5	31.502942726906262	32.0	32.0	32.0	32.0	32.0
6	35.05765450091476	36.0	36.0	36.0	36.0	36.0
7	35.09123693757946	36.0	36.0	36.0	36.0	36.0
8	35.06841762535272	36.0	36.0	36.0	36.0	36.0
9	35.17005178455146	36.0	36.0	36.0	36.0	36.0
10	35.03176532605662	36.0	36.0	36.0	36.0	36.0
11	35.16887035256907	36.0	36.0	36.0	36.0	36.0
12	35.09948215448541	36.0	36.0	36.0	36.0	36.0
13	35.12721944866507	36.0	36.0	36.0	36.0	36.0
14	35.072563490340784	36.0	36.0	36.0	36.0	36.0
15	35.05300009302614	36.0	36.0	36.0	36.0	36.0
16	35.0379794722317	36.0	36.0	36.0	36.0	36.0
17	35.03552358212658	36.0	36.0	36.0	36.0	36.0
18	35.01046854166021	36.0	36.0	36.0	36.0	36.0
19	34.98274985270861	36.0	36.0	36.0	36.0	36.0
20	34.961465471797574	36.0	36.0	36.0	36.0	36.0
21	34.929501689974884	36.0	36.0	36.0	36.0	36.0
22	34.9052342708301	36.0	36.0	36.0	36.0	36.0
23	34.878417935439856	36.0	36.0	36.0	32.0	36.0
24	34.86384073924773	36.0	36.0	36.0	32.0	36.0
25	34.821206859127415	36.0	36.0	36.0	32.0	36.0
26	34.78515612887222	36.0	36.0	36.0	32.0	36.0
27	34.721737108127385	36.0	36.0	36.0	32.0	36.0
28	34.6738224441068	36.0	36.0	36.0	32.0	36.0
29	34.59597816986573	36.0	36.0	36.0	32.0	36.0
30	34.523529411764706	36.0	36.0	36.0	32.0	36.0
31	34.47678067536978	36.0	36.0	36.0	32.0	36.0
32	34.3947316195851	36.0	36.0	36.0	32.0	36.0
33	34.35489782628919	36.0	36.0	36.0	32.0	36.0
34	34.25032714192688	36.0	36.0	36.0	32.0	36.0
35	34.1468851747341	36.0	36.0	36.0	32.0	36.0
36	34.03196998356538	36.0	36.0	36.0	32.0	36.0
37	33.92657756829669	36.0	36.0	36.0	32.0	36.0
38	33.83610344506806	36.0	36.0	36.0	32.0	36.0
39	33.62056187788769	36.0	36.0	36.0	27.0	36.0
40	33.583708021954166	36.0	36.0	36.0	21.0	36.0
41	33.531402524109275	36.0	36.0	36.0	21.0	36.0
42	33.407609538280255	36.0	36.0	36.0	21.0	36.0
43	33.35416912152315	36.0	36.0	36.0	21.0	36.0
44	33.221225464355484	36.0	36.0	36.0	14.0	36.0
45	33.00729945114577	36.0	36.0	36.0	14.0	36.0
46	32.79367112158516	36.0	36.0	36.0	14.0	36.0
47	32.668848646469655	36.0	36.0	36.0	14.0	36.0
48	32.52616205153648	36.0	36.0	36.0	14.0	36.0
49	32.40666067164873	36.0	36.0	36.0	14.0	36.0
50	31.902939626034915	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	3.0
21	16.0
22	44.0
23	188.0
24	461.0
25	1153.0
26	2305.0
27	4257.0
28	6861.0
29	9389.0
30	12190.0
31	15643.0
32	21105.0
33	29686.0
34	53545.0
35	165642.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.81270739135019	21.20400176146971	13.463768924945263	22.519521922234834
2	11.450348540100718	23.060703033911338	45.37316117188857	20.115787254099377
3	17.986095606658232	30.67276921931979	30.892002183026968	20.449132990995015
4	9.348817017581942	18.419485875531024	40.32404105553661	31.90765605135043
5	8.848646469657973	43.36103445068064	35.77754348972061	12.012775589940773
6	26.395008822006332	40.74247493712964	20.296196149326022	12.566320091538005
7	24.41471053365996	34.79022605352104	23.3232038202735	17.471859592545506
8	25.00883748333282	38.585072405345905	20.59753790815219	15.808552203169091
9	27.67486138847199	14.259312089902135	22.52700909192394	35.53881742970194
10	15.57794398551264	29.249460445039816	35.01897745032373	20.153618119123813
11	31.111352289993487	22.671400663586468	28.73329405562963	17.483952990790414
12	24.91681886824047	27.248061174179583	33.28051499430988	14.554604963270066
13	29.552945991956314	23.30188006412622	28.761601170892654	18.383572773024817
14	19.961921181807753	25.416680879037735	30.405068079841485	24.21632985931303
15	20.87660595836525	35.01834273469552	27.677217486223565	16.427833820715662
16	17.82360894048771	28.39020366649302	34.317866091141376	19.4683213018779
17	16.615088839964027	30.36621290582654	32.919780458308786	20.098917795900647
18	17.13856906747207	26.373302655284363	39.46150101243763	17.02662726480593
19	21.984867747837143	26.722068901361283	32.22611553846631	19.066947812335265
20	21.993618386983744	27.611794510820527	34.216670956218664	16.177916145977072
21	21.529969921547956	25.380942044714565	30.755992433873917	22.33309559986356
22	21.074827358452534	30.708954413949	30.568794594544308	17.647423633054153
23	18.28482476758681	30.779940834640882	33.340362062229055	17.59487233554325
24	21.210401627347373	29.162015330803005	33.22945349904492	16.398129542804693
25	19.84780640279828	29.45851577132509	33.69500502350504	16.99867280237159
26	15.890160324991474	30.730920705802088	36.00024808509319	17.37867088411325
27	17.580240022327658	31.75148075789996	33.617390765032404	17.050888454739976
28	16.30653203999107	30.932297997965712	33.081011684735415	19.680158277307797
29	18.2364524381735	29.0865958601442	32.56872625784945	20.108225443832854
30	17.741840452748274	29.25653151407086	33.95177920769052	19.049848825490347
31	20.110338924707175	28.219846741156296	32.692015468537285	18.97779886559925
32	16.249530974302044	32.20818849096215	33.215392105483495	18.32688842925232
33	16.51280270163829	32.047532956451896	34.55318462745487	16.886479714454943
34	17.284857957100282	31.375339950941626	34.900286225513454	16.43951586644463
35	17.725185205858367	32.648947683415045	32.575764623432846	17.050102487293746
36	16.60112760269682	31.339353830313783	34.22224565364362	17.83727291334578
37	18.0563092886448	31.80336714536901	29.82054088920175	20.319782676784435
38	18.08508988739654	34.715421185328985	30.03017437875823	17.169314548516247
39	19.108015579647226	31.5121933067057	30.340618720385027	19.03917239326205
40	20.396360749680603	31.9073194329021	31.91445156969028	15.781868247727019
41	19.79527158610253	30.230776864014686	32.76212803433434	17.211823515548446
42	18.2701103624067	34.98354952043984	30.87139477119724	15.87494534595622
43	15.791595346183424	34.76191362159212	31.515591277814988	17.930899754409467
44	15.878469519701568	33.85128702862441	31.35382201453082	18.916421437143203
45	16.900010853217978	33.97894475712049	30.777865637161415	18.343178752500116
46	18.55175557464332	31.107300449320434	29.906321805220053	20.434622170816187
47	21.41986659679175	31.53643160372239	28.847901116654935	18.195800682830924
48	17.544343835276607	34.143822872736294	27.972587447283555	20.339245844703548
49	19.090632829748706	31.73389397434943	29.86498722433083	19.310485971571033
50	16.432600383891444	35.81043576950388	27.833990207357196	19.922973639247473
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	75.0
1	51.0
2	27.0
3	129.0
4	231.0
5	283.5
6	336.0
7	322.0
8	308.0
9	427.0
10	546.0
11	848.0
12	1150.0
13	1928.5
14	2707.0
15	3743.0
16	4779.0
17	5648.0
18	6517.0
19	6573.5
20	6630.0
21	6933.5
22	7237.0
23	7366.0
24	7495.0
25	7138.5
26	6782.0
27	8335.0
28	9888.0
29	11726.0
30	13564.0
31	15489.0
32	17414.0
33	21372.5
34	25331.0
35	29035.0
36	32739.0
37	33188.5
38	33638.0
39	29826.0
40	26014.0
41	23941.5
42	21869.0
43	18825.0
44	15781.0
45	15906.5
46	16032.0
47	15950.5
48	15869.0
49	13968.0
50	12067.0
51	10709.0
52	9351.0
53	7880.0
54	6409.0
55	5742.0
56	5075.0
57	4907.5
58	4740.0
59	4008.0
60	3276.0
61	2913.5
62	2551.0
63	2235.0
64	1919.0
65	1686.5
66	1454.0
67	1177.0
68	900.0
69	781.0
70	662.0
71	531.5
72	401.0
73	343.5
74	286.0
75	244.0
76	202.0
77	150.0
78	98.0
79	78.0
80	58.0
81	39.5
82	21.0
83	19.5
84	18.0
85	16.0
86	14.0
87	11.0
88	8.0
89	5.5
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	3.5
96	5.0
97	6.5
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009922788303513286
2	6.201742689695804E-4
3	6.201742689695804E-4
4	0.0
5	0.0
6	3.100871344847902E-4
7	0.0
8	0.0
9	0.0018605228069087415
10	6.201742689695804E-4
11	0.0
12	3.100871344847902E-4
13	3.100871344847902E-4
14	3.100871344847902E-4
15	0.005891655555211014
16	6.201742689695804E-4
17	0.0
18	3.100871344847902E-4
19	0.0
20	3.100871344847902E-4
21	0.0
22	3.100871344847902E-4
23	0.0012403485379391607
24	6.201742689695804E-4
25	0.0018605228069087415
26	0.006201742689695805
27	0.006201742689695805
28	0.005581568420726224
29	0.004651307017271854
30	0.004651307017271854
31	0.008992526900058917
32	0.004031132748302273
33	0.005891655555211014
34	0.005271481286241434
35	0.0034109584793326924
36	0.011163136841452448
37	0.006511829824180595
38	0.009612701169028497
39	0.005581568420726224
40	0.0018605228069087415
41	0.004341219882787063
42	0.0021706099413935317
43	6.201742689695804E-4
44	9.302614034543708E-4
45	0.0015504356724239513
46	9.302614034543708E-4
47	0.002790784210363112
48	0.0031008713448479027
49	6.201742689695804E-4
50	9.302614034543708E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	322490.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.94887122256109
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.29034387866746	34.29164842527125
2	12.483466957097956	11.222354973313852
3	4.34137288937418	5.854194328007938
4	2.018734064901417	3.6295927006340425
5	1.1412315509967654	2.564853501043863
6	0.695914744305959	1.8768349334214043
7	0.4924328374597038	1.5494010137715606
8	0.3605971976964739	1.2966749601980168
9	0.28023949375933566	1.1336804024817682
>10	1.5407508875692522	13.43888955875302
>50	0.19371606138015618	6.211427702094746
>100	0.15012994756962103	12.978568896912199
>500	0.009685803069007808	3.0886101901073904
>1k	0.0013836861527154012	0.8632684139889364
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1708	0.5296288257000217	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1068	0.33117305962975596	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	944	0.29272225495364196	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	888	0.2753573754224937	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	834	0.25861267016031503	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	828	0.25675214735340635	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	768	0.23814691928431891	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	742	0.23008465378771434	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	733	0.22729386957735126	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	679	0.21054916431517257	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	662	0.20527768302893112	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	609	0.18884306490123726	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	592	0.18357158361499581	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	571	0.1770597537908152	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	553	0.171478185370089	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	529	0.16403609414245404	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	483	0.14977208595615368	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	471	0.14605104034233618	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	460	0.1426400818630035	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	457	0.14170982045954914	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	449	0.13922912338367083	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	412	0.12775589940773358	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	408	0.1265155508697944	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA	391	0.12124406958355298	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	373	0.11566250116282675	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	364	0.11287171695246365	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT	360	0.11163136841452449	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT	356	0.11039101987658533	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	353	0.10946075847313094	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	353	0.10946075847313094	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	353	0.10946075847313094	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	351	0.10884058420416137	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	349	0.1082204099351918	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	349	0.1082204099351918	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG	345	0.10698006139725263	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC	344	0.10666997426276785	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	341	0.10573971285931347	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	339	0.1051195385903439	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA	336	0.10418927718688951	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	331	0.10263884151446556	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	330	0.10232875437998078	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	329	0.10201866724549598	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	327	0.10139849297652641	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC	327	0.10139849297652641	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	3.1008713448479023E-4	0.0
17	0.0	0.0	0.0	6.201742689695805E-4	0.0
18	0.0	0.0	0.0	6.201742689695805E-4	0.0
19	0.0	0.0	0.0	6.201742689695805E-4	0.0
20	0.0	0.0	0.0	0.001240348537939161	0.0
21	0.0	0.0	0.0	0.0018605228069087413	0.0
22	0.0	0.0	0.0	0.0037210456138174826	0.0
23	0.0	0.0	0.0	0.009302614034543707	0.0
24	0.0	0.0	0.0	0.017674966665633042	0.0
25	0.0	0.0	0.0	0.020775838010480944	0.0
26	0.0	0.0	0.0	0.0254271450277528	0.0
27	0.0	0.0	0.0	0.03379949765884213	0.0
28	0.0	0.0	0.0	0.04899376724859686	0.0
29	0.0	0.0	0.0	0.07318056373841049	0.0
30	0.0	0.0	0.0	0.11225154268349406	0.0
31	0.0	0.0	0.0	0.1903935005736612	0.0
32	0.0	0.0	0.0	0.273496852615585	0.0
33	0.0	0.0	0.0	0.3435765450091476	0.0
34	0.0	0.0	0.0	0.42295885143725387	0.0
35	0.0	0.0	0.0	0.5110235976309343	0.0
36	0.0	0.0	0.0	0.6251356631213371	0.0
37	0.0	0.0	0.0	0.7832801017085801	0.0
38	0.0	0.0	0.0	0.9901082204099352	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAGA	25	0.0023505276	35.199135	20
GGGCGTG	25	0.0023505276	35.199135	7
ATACGTG	25	0.0023505276	35.199135	8
GGCCTAC	45	4.0610874E-5	29.33261	22
GTGCGCG	30	0.0057395413	29.332607	12
TAGGACC	445	0.0	27.684711	4
AATGAAC	140	0.0	26.713625	25
CATAATG	140	0.0	26.713625	22
CTCTAGG	50	8.3242165E-5	26.39935	1
TAACTAG	140	0.0	25.146135	32
GTCCTAA	555	0.0	24.972357	1
TGCATAA	150	0.0	24.932718	20
GGACCTG	650	0.0	24.707085	6
CTAGGAC	90	1.5043042E-8	24.44384	3
CTGTAGG	1035	0.0	24.231285	1
TAGGACA	300	0.0	24.199404	4
ACTAACT	155	0.0	24.13218	30
AGATTAT	55	1.5903532E-4	23.999409	5
GCCTACA	55	1.5903532E-4	23.999409	23
AGGACCT	690	0.0	23.912455	5
>>END_MODULE