FastQCFastQC Report
Thu 2 Feb 2017
SRR4064028_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064028_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260233
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT4670.17945456571610827No Hit
TATCAACGCAGAGTACTTTTTTTTT2960.11374422152455683No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG2950.1133599505058928No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC250.006019225318.9998078
TAGACTG250.006025917418.9961555
TTTAGGC250.006032615418.9925043
TCCAATA250.006032615418.9925042
GGTATCA804.2564352E-1016.6216351
GACCGTG350.00216381616.285557
GAACAGA456.7360915E-414.7776296
TAGAGTG400.005270551414.2471165
GTATCAA3350.013.3256611
TATACAC655.4386262E-513.1486563
ACAGATT604.0720458E-412.6665388
GTGTTCA550.003056879412.0907869
CTTGGGC550.003065701312.0861393
CTTATAG550.003065701312.0861393
TCAAGAC550.003065701312.0861393
AACAGAT657.984746E-411.6921897
CTTCACT657.997646E-411.689944
TGGAGAG752.0650649E-411.3976935
TGGGCTG600.005862218311.0810915
TGCCCTG700.001484304910.85703310
Produced by FastQC (version 0.11.5)