##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064028_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 260233 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.29448609515319 32.0 32.0 32.0 32.0 32.0 2 30.93861270476842 32.0 32.0 32.0 32.0 32.0 3 30.948622964804617 32.0 32.0 32.0 32.0 32.0 4 30.98052130206392 32.0 32.0 32.0 32.0 32.0 5 30.91164072196839 32.0 32.0 32.0 32.0 32.0 6 34.56659993159976 36.0 36.0 36.0 32.0 36.0 7 34.50657679848444 36.0 36.0 36.0 32.0 36.0 8 34.48649863775924 36.0 36.0 36.0 32.0 36.0 9 34.608796732159256 36.0 36.0 36.0 32.0 36.0 10 34.34015286301123 36.0 36.0 36.0 32.0 36.0 11 34.57498088251682 36.0 36.0 36.0 32.0 36.0 12 34.43089846406874 36.0 36.0 36.0 32.0 36.0 13 34.4784558453386 36.0 36.0 36.0 32.0 36.0 14 34.38319505981178 36.0 36.0 36.0 32.0 36.0 15 34.33911148855064 36.0 36.0 36.0 32.0 36.0 16 34.34779217086226 36.0 36.0 36.0 32.0 36.0 17 34.28536734388029 36.0 36.0 36.0 32.0 36.0 18 34.26431313476769 36.0 36.0 36.0 32.0 36.0 19 34.27548005057007 36.0 36.0 36.0 32.0 36.0 20 34.25776515660965 36.0 36.0 36.0 32.0 36.0 21 34.26497408091979 36.0 36.0 36.0 32.0 36.0 22 34.25910626246479 36.0 36.0 36.0 32.0 36.0 23 34.193653379855746 36.0 36.0 36.0 32.0 36.0 24 34.17668012896135 36.0 36.0 36.0 32.0 36.0 25 33.78360546125972 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 5.0 5 6.0 6 12.0 7 4.0 8 18.0 9 14.0 10 38.0 11 11.0 12 19.0 13 15.0 14 20.0 15 53.0 16 77.0 17 96.0 18 99.0 19 163.0 20 213.0 21 299.0 22 448.0 23 773.0 24 1199.0 25 1783.0 26 2630.0 27 3439.0 28 4848.0 29 6670.0 30 8568.0 31 11585.0 32 16668.0 33 23664.0 34 53094.0 35 123701.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41834718495568 17.74596560287232 12.25580268934181 25.579884522830188 2 16.091247271497526 20.15364159006364 38.685553540135885 25.06955759830295 3 18.42353022553386 23.688548843893987 29.257532846322633 28.63038808424952 4 12.753823687648913 16.313888248405195 36.41610944585351 34.51617861809238 5 14.251235578512079 36.65498343594592 34.47706013020653 14.616720855335469 6 33.007287038602854 36.170384491214044 17.430473350039204 13.391855120143898 7 29.553535392362246 31.01228114008956 21.252330341527166 18.181853126021025 8 26.86072572243558 34.29670891526062 19.874907256839926 18.96765810546387 9 26.73467190612047 15.218895869700628 18.88741069986696 39.15902152431194 10 15.267395717977115 28.033868586194167 33.37986034206965 23.318875353759076 11 35.52194960645665 22.11571366500944 22.921641231500388 19.440695497033524 12 23.63667826722298 24.40154588628891 30.02672614355208 21.93504970293603 13 29.2893867552674 20.999603911660763 25.233903623631477 24.477105709440362 14 22.797315745957274 19.962697329205685 26.683715653662006 30.556271271175035 15 24.360793542755406 27.83744601978858 23.168710512249614 24.633049925206404 16 24.455570809443053 26.958618382344458 24.59439400406856 23.99141680414393 17 23.181133004168398 26.778105917278083 25.97249780813068 24.068263270422836 18 23.23037694269133 26.902116432639904 26.76789237461204 23.099614250056728 19 24.732514403070603 25.733998938518692 26.044751436461112 23.488735221949586 20 24.93673320256913 25.71862620668436 26.095150186531285 23.249490404215223 21 25.266234640309214 25.451993154241105 25.47122281406842 23.810549391381265 22 24.993173260719903 25.470852707811716 25.76776779086717 23.768206240601213 23 24.680954175865597 25.777902566982313 25.905982445748748 23.635160811403342 24 24.24916060597902 25.69526439469403 25.589114222969204 24.46646077635774 25 24.051382639129265 25.874004845967463 25.885927464328297 24.18868505057498 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 14.0 1 14.0 2 35.5 3 57.0 4 57.0 5 57.0 6 134.0 7 211.0 8 211.0 9 211.0 10 306.0 11 401.0 12 401.0 13 401.0 14 636.0 15 871.0 16 871.0 17 871.0 18 1693.0 19 2515.0 20 2515.0 21 2515.0 22 3407.0 23 4299.0 24 4299.0 25 4299.0 26 6444.0 27 8589.0 28 8589.0 29 8589.0 30 11340.5 31 14092.0 32 14092.0 33 14092.0 34 16562.5 35 19033.0 36 19033.0 37 19033.0 38 21791.0 39 24549.0 40 24549.0 41 24549.0 42 27079.0 43 29609.0 44 29609.0 45 29609.0 46 31727.5 47 33846.0 48 33846.0 49 33846.0 50 34391.0 51 34936.0 52 34936.0 53 34936.0 54 32900.5 55 30865.0 56 30865.0 57 30865.0 58 27089.5 59 23314.0 60 23314.0 61 23314.0 62 19488.5 63 15663.0 64 15663.0 65 15663.0 66 12133.0 67 8603.0 68 8603.0 69 8603.0 70 6470.0 71 4337.0 72 4337.0 73 4337.0 74 3299.5 75 2262.0 76 2262.0 77 2262.0 78 1657.0 79 1052.0 80 1052.0 81 1052.0 82 766.5 83 481.0 84 481.0 85 481.0 86 335.5 87 190.0 88 190.0 89 190.0 90 142.5 91 95.0 92 95.0 93 95.0 94 64.5 95 34.0 96 34.0 97 34.0 98 174.5 99 315.0 100 315.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03650574677308412 2 0.006532607317288738 3 0.0023056261119842603 4 0.004995523242632564 5 0.011912401578585346 6 0.017292195839881952 7 0.029973139455795383 8 0.040732727978388596 9 0.059562007892926726 10 0.06494180215422334 11 0.061483362986246945 12 0.07224295150884015 13 0.07301149354616825 14 0.07608566169548059 15 0.07070586743418399 16 0.07301149354616825 17 0.06955305437819186 18 0.08453962410608955 19 0.0841553530874255 20 0.08569243716208166 21 0.08377108206876145 22 0.0868452502180738 23 0.09184077346070636 24 0.08607670818074573 25 0.08569243716208166 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 260233.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.08539349439322 #Duplication Level Percentage of deduplicated Percentage of total 1 59.78177049887545 27.550664172321838 2 17.610232370679938 16.231489766609723 3 8.564418047341322 11.840837272866231 4 4.693528147185163 8.652123661601545 5 2.908885211688995 6.702855980535433 6 1.8371957925344153 5.080073461511529 7 1.1994934728220865 3.869539008227349 8 0.8352595042214453 3.079461033758169 9 0.5621224102675667 2.33150692222778 >10 1.9786962111776736 13.257238714849162 >50 0.019210637168731073 0.592401096066627 >100 0.00918769603721921 0.8118089094246371 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 467 0.17945456571610827 No Hit TATCAACGCAGAGTACTTTTTTTTT 296 0.11374422152455683 No Hit GGGGGGGGGGGGGGGGGGGGGGGGG 295 0.1133599505058928 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.842710186640434E-4 2 0.0 0.0 0.0 0.0 3.842710186640434E-4 3 0.0 0.0 0.0 0.0 3.842710186640434E-4 4 0.0 0.0 0.0 0.0 3.842710186640434E-4 5 0.0 0.0 0.0 0.0 3.842710186640434E-4 6 0.0 0.0 0.0 0.0 7.685420373280868E-4 7 0.0 0.0 0.0 0.0 7.685420373280868E-4 8 0.0 0.0 0.0 0.0 7.685420373280868E-4 9 0.0 0.0 0.0 0.0 7.685420373280868E-4 10 0.0 0.0 0.0 0.0 7.685420373280868E-4 11 0.0 0.0 0.0 0.0 7.685420373280868E-4 12 0.0 0.0 0.0 0.0 0.0023056261119842603 13 0.0 0.0 0.0 0.0 0.0026898971306483035 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTGC 25 0.0060192253 18.999807 8 TAGACTG 25 0.0060259174 18.996155 5 TTTAGGC 25 0.0060326154 18.992504 3 TCCAATA 25 0.0060326154 18.992504 2 GGTATCA 80 4.2564352E-10 16.621635 1 GACCGTG 35 0.002163816 16.28555 7 GAACAGA 45 6.7360915E-4 14.777629 6 TAGAGTG 40 0.0052705514 14.247116 5 GTATCAA 335 0.0 13.325661 1 TATACAC 65 5.4386262E-5 13.148656 3 ACAGATT 60 4.0720458E-4 12.666538 8 GTGTTCA 55 0.0030568794 12.090786 9 CTTGGGC 55 0.0030657013 12.086139 3 CTTATAG 55 0.0030657013 12.086139 3 TCAAGAC 55 0.0030657013 12.086139 3 AACAGAT 65 7.984746E-4 11.692189 7 CTTCACT 65 7.997646E-4 11.68994 4 TGGAGAG 75 2.0650649E-4 11.397693 5 TGGGCTG 60 0.0058622183 11.081091 5 TGCCCTG 70 0.0014843049 10.857033 10 >>END_MODULE