FastQCFastQC Report
Thu 2 Feb 2017
SRR4064028_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064028_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260233
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATA207.8496337E-443.9950032
CCGCGCG250.002348504735.2027721
GGTATCA756.002665E-1132.2630041
GTATCAA2950.032.0641561
TAGGACG502.3541452E-630.7965034
GAATTAC300.00574002229.3300021
TCTACAC551.5901611E-423.9972743
TGGGGGG651.8052062E-523.6941719
TAGAGTG500.002576747421.9975035
TATAACC500.002576747421.9975033
CTAGGAC602.866597E-421.9975013
TATCAAC4350.021.2389662
AGGATAC654.921638E-420.3053875
GTCTAAG550.004477266719.9977281
CCTTTAC550.004477266719.9977281
TCTCCCG550.004477266719.99772810
ATCAACG4700.019.6573433
CAACGCA4800.019.2478145
TCAACGC4800.019.2478144
CTGTAGG708.1061316E-418.8550031