##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064028_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	260233
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33294393870109	32.0	32.0	32.0	32.0	32.0
2	31.474797585240918	32.0	32.0	32.0	32.0	32.0
3	31.525609742038863	32.0	32.0	32.0	32.0	32.0
4	31.585521436558775	32.0	32.0	32.0	32.0	32.0
5	31.568225398008707	32.0	32.0	32.0	32.0	32.0
6	35.15128365733785	36.0	36.0	36.0	36.0	36.0
7	35.16402224160656	36.0	36.0	36.0	36.0	36.0
8	35.123327940730036	36.0	36.0	36.0	36.0	36.0
9	35.18562595827585	36.0	36.0	36.0	36.0	36.0
10	35.083060180684235	36.0	36.0	36.0	36.0	36.0
11	35.196673750062445	36.0	36.0	36.0	36.0	36.0
12	35.124987991530666	36.0	36.0	36.0	36.0	36.0
13	35.14163461205919	36.0	36.0	36.0	36.0	36.0
14	35.09591020354836	36.0	36.0	36.0	36.0	36.0
15	35.081711389408724	36.0	36.0	36.0	36.0	36.0
16	35.08861289690393	36.0	36.0	36.0	36.0	36.0
17	35.0701832588488	36.0	36.0	36.0	36.0	36.0
18	35.06403492255018	36.0	36.0	36.0	36.0	36.0
19	35.05316389543217	36.0	36.0	36.0	36.0	36.0
20	35.04333424277475	36.0	36.0	36.0	36.0	36.0
21	35.05287953487836	36.0	36.0	36.0	36.0	36.0
22	35.04198545149924	36.0	36.0	36.0	36.0	36.0
23	34.972889679633255	36.0	36.0	36.0	36.0	36.0
24	34.96223000157551	36.0	36.0	36.0	36.0	36.0
25	34.944945491156005	36.0	36.0	36.0	36.0	36.0
26	34.89879069910427	36.0	36.0	36.0	36.0	36.0
27	34.90082733550318	36.0	36.0	36.0	36.0	36.0
28	34.86096690273716	36.0	36.0	36.0	32.0	36.0
29	34.87313676589825	36.0	36.0	36.0	32.0	36.0
30	34.84812456529341	36.0	36.0	36.0	32.0	36.0
31	34.831293494675926	36.0	36.0	36.0	32.0	36.0
32	34.78405121564137	36.0	36.0	36.0	32.0	36.0
33	34.781803230182184	36.0	36.0	36.0	32.0	36.0
34	34.7661211299105	36.0	36.0	36.0	32.0	36.0
35	34.731425299635326	36.0	36.0	36.0	32.0	36.0
36	34.700991034957134	36.0	36.0	36.0	32.0	36.0
37	34.699296399764826	36.0	36.0	36.0	32.0	36.0
38	34.68759150453632	36.0	36.0	36.0	32.0	36.0
39	34.67980617369819	36.0	36.0	36.0	32.0	36.0
40	34.662767596730625	36.0	36.0	36.0	32.0	36.0
41	34.644595420258	36.0	36.0	36.0	32.0	36.0
42	34.60262533959951	36.0	36.0	36.0	32.0	36.0
43	34.57445058851106	36.0	36.0	36.0	32.0	36.0
44	34.54743249318879	36.0	36.0	36.0	32.0	36.0
45	34.52238955090246	36.0	36.0	36.0	32.0	36.0
46	34.510461778483126	36.0	36.0	36.0	32.0	36.0
47	34.47033619871423	36.0	36.0	36.0	32.0	36.0
48	34.437796128853755	36.0	36.0	36.0	32.0	36.0
49	34.41154657556882	36.0	36.0	36.0	32.0	36.0
50	33.86813739994543	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	4.0
21	4.0
22	23.0
23	76.0
24	184.0
25	451.0
26	897.0
27	1713.0
28	2797.0
29	4281.0
30	6242.0
31	8782.0
32	12182.0
33	19020.0
34	39328.0
35	164247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.694465795541895	18.508455034588778	12.751345119139124	26.045734050730207
2	15.378526166935016	21.093789027525332	38.03091844616171	25.496766359377943
3	17.67492977447134	24.51495390669131	29.49126359839067	28.318852720446674
4	12.361230128384948	17.06970292007547	36.29209208670691	34.276974864832674
5	13.815695934028351	37.12596019720789	34.27466923872068	14.783674630043075
6	32.92318806607925	36.64408433980318	17.28835312969531	13.144374464422267
7	29.327179873421127	31.09136812010775	21.28899870500667	18.292453301464455
8	26.693002040479108	34.59591981032383	19.71425607052142	18.99682207867565
9	26.738935791662023	15.017811098600859	18.727207750037465	39.51604535969965
10	15.06622961906921	28.299088117864514	33.37842147937794	23.256260783688337
11	35.72759796028943	22.07406439613731	22.8564401901373	19.341897453435962
12	23.426878427243487	24.54511568567926	29.98950932056519	22.038496566512062
13	29.373246743265575	20.96337854974446	25.354494101371866	24.308880605618107
14	22.694836510638623	20.269299199557317	26.416145655206336	30.61971863459772
15	24.433650117785405	28.089416991072902	22.855748427286247	24.621184463855446
16	24.435119431891543	27.077377109656	24.47738940637585	24.01011405207661
17	23.26030903075321	26.765629263006613	26.02629182309699	23.947769883143184
18	23.347538551989945	26.951232165021345	26.551206034592077	23.15002324839663
19	24.640994800813118	25.753075128826858	26.32525467561762	23.280675394742403
20	24.869827191994805	25.74789321794867	26.080674477675604	23.301605112380923
21	25.2193226839025	25.57554191820407	25.622422982481087	23.58271241541234
22	24.943896215684465	25.6471148821052	25.685542131636385	23.72344677057395
23	24.52292203051147	25.663835837528342	25.955885178495947	23.857356953464244
24	24.429355344461865	25.564880568108457	25.572950290510004	24.43281379691967
25	24.44654515830288	25.650869042266617	25.772684827594155	24.129900971836342
26	23.624513386904006	26.493657216861312	26.331484876084193	23.55034452015049
27	24.353022558702587	25.92444564005995	25.899081511087196	23.823450290150262
28	24.21815554650332	25.74687377506552	26.028560668362683	24.006410010068482
29	24.386682242990652	25.850421175602555	25.793162813575996	23.969733767830792
30	23.99853969717931	26.324264084236415	25.76051033740681	23.916685881177465
31	24.017816310609465	26.13955597572739	25.489698744480442	24.352928969182702
32	23.849050803166552	26.062946737376063	25.773191914533854	24.314810544923525
33	23.95117995857367	26.056313671840474	25.817286076066114	24.17522029351974
34	24.531941711756026	25.47958681751107	26.18167424987703	23.806797220855877
35	24.18233591957667	25.965421965006936	26.05688198199237	23.795360133424026
36	24.478776349416805	25.692050498645298	25.802732460944256	24.02644069099364
37	24.037061090850397	25.366326056129644	25.900107217382285	24.696505635637674
38	23.764358650161984	25.954521173364487	26.00793977149138	24.27318040498215
39	23.97691157764481	25.80922845163844	26.131651659960724	24.082208310756023
40	24.44337701264266	26.005456711370712	25.904776543826614	23.64638973216001
41	24.30166202324911	25.442597751945435	26.44403881256605	23.811701412239408
42	24.024594101258526	25.65472187530022	26.029397636660583	24.29128638678067
43	23.785295971225235	25.079545629217453	26.587838357132977	24.547320042424335
44	23.74792970806482	25.338067625053316	26.21652467634276	24.697477990539102
45	23.593743995696116	25.474772316796678	26.437766591092494	24.49371709641471
46	23.855252236465923	25.097605213809217	26.658520089766053	24.388622459958807
47	23.527829441874047	25.27629617105	26.593218254761208	24.602656132314742
48	23.377371976666435	25.645784817812213	26.42626025070516	24.550582954816196
49	23.558119070217842	25.405309856935897	26.638435555828817	24.39813551701744
50	23.407574779427588	25.85615911955486	26.392219250514927	24.34404685050263
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	14.5
4	29.0
5	37.5
6	46.0
7	43.0
8	40.0
9	55.0
10	70.0
11	103.5
12	137.0
13	238.5
14	340.0
15	443.0
16	546.0
17	542.5
18	539.0
19	530.0
20	521.0
21	647.0
22	773.0
23	1062.5
24	1352.0
25	1612.0
26	1872.0
27	2432.0
28	2992.0
29	3494.0
30	3996.0
31	4712.5
32	5429.0
33	6429.5
34	7430.0
35	8831.0
36	10232.0
37	11178.5
38	12125.0
39	13009.5
40	13894.0
41	14573.0
42	15252.0
43	16101.0
44	16950.0
45	17837.0
46	18724.0
47	18852.5
48	18981.0
49	19179.5
50	19378.0
51	19669.0
52	19960.0
53	19323.0
54	18686.0
55	17923.0
56	17160.0
57	16110.5
58	15061.0
59	13551.5
60	12042.0
61	10141.0
62	8240.0
63	6959.5
64	5679.0
65	4789.5
66	3900.0
67	3296.0
68	2692.0
69	2215.5
70	1739.0
71	1434.5
72	1130.0
73	977.5
74	825.0
75	691.0
76	557.0
77	482.0
78	407.0
79	284.5
80	162.0
81	148.0
82	134.0
83	110.0
84	86.0
85	69.5
86	53.0
87	40.5
88	28.0
89	22.0
90	16.0
91	15.5
92	15.0
93	10.5
94	6.0
95	5.5
96	5.0
97	3.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012680943615913431
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	7.685420373280868E-4
10	7.685420373280868E-4
11	0.0
12	7.685420373280868E-4
13	0.0011528130559921301
14	7.685420373280868E-4
15	0.005379794261296608
16	3.842710186640434E-4
17	0.0
18	0.0
19	0.0
20	7.685420373280868E-4
21	0.0
22	3.842710186640434E-4
23	0.0011528130559921301
24	3.842710186640434E-4
25	0.0015370840746561736
26	0.006148336298624694
27	0.008838233429272998
28	0.005764065279960651
29	0.0034584391679763904
30	0.004995523242632564
31	0.008453962410608955
32	0.004995523242632564
33	0.0046112522239685205
34	0.0034584391679763904
35	0.003842710186640434
36	0.010759588522593216
37	0.005379794261296608
38	0.009222504447937041
39	0.006148336298624694
40	0.0011528130559921301
41	0.003074168149312347
42	0.003074168149312347
43	0.001921355093320217
44	0.0015370840746561736
45	0.0011528130559921301
46	3.842710186640434E-4
47	0.001921355093320217
48	0.002689897130648304
49	0.0
50	3.842710186640434E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	260233.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.65759022616935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.28768688752534	32.56659298936539
2	16.284336520781643	16.498504931496015
3	7.512854958184508	11.417493837010664
4	4.060098288971908	8.226991816028407
5	2.4525336088324172	6.211972128607044
6	1.5766652178047222	4.792203631644338
7	1.038909444653923	3.6840054250562915
8	0.7154353061179485	2.8993782856525647
9	0.5078175656098439	2.3152332733482908
>10	1.5553004236653771	11.07724955642529
>50	0.007601616229430139	0.25299727092464536
>100	7.60161622943014E-4	0.05737685444105351
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.685420373280868E-4
2	0.0	0.0	0.0	0.0	7.685420373280868E-4
3	0.0	0.0	0.0	0.0	7.685420373280868E-4
4	0.0	0.0	0.0	0.0	7.685420373280868E-4
5	0.0	0.0	0.0	0.0	7.685420373280868E-4
6	0.0	0.0	0.0	0.0	0.0011528130559921301
7	0.0	0.0	0.0	0.0	0.0011528130559921301
8	0.0	0.0	0.0	0.0	0.0011528130559921301
9	0.0	0.0	0.0	0.0	0.0011528130559921301
10	0.0	0.0	0.0	0.0	0.0015370840746561736
11	0.0	0.0	0.0	0.0	0.0015370840746561736
12	0.0	0.0	0.0	0.0	0.0038427101866404336
13	0.0	0.0	0.0	0.0	0.004226981205304477
14	0.0	0.0	0.0	0.0	0.004226981205304477
15	0.0	0.0	0.0	0.0	0.005764065279960651
16	0.0	0.0	0.0	0.0	0.005764065279960651
17	0.0	0.0	0.0	3.842710186640434E-4	0.005764065279960651
18	0.0	0.0	0.0	3.842710186640434E-4	0.005764065279960651
19	0.0	0.0	0.0	3.842710186640434E-4	0.005764065279960651
20	0.0	0.0	0.0	3.842710186640434E-4	0.005764065279960651
21	0.0	0.0	0.0	3.842710186640434E-4	0.005764065279960651
22	0.0	0.0	0.0	7.685420373280868E-4	0.005764065279960651
23	0.0	0.0	0.0	7.685420373280868E-4	0.005764065279960651
24	0.0	0.0	0.0	0.0011528130559921301	0.005764065279960651
25	0.0	0.0	0.0	0.0011528130559921301	0.005764065279960651
26	0.0	0.0	0.0	0.0015370840746561736	0.006148336298624694
27	0.0	0.0	0.0	0.0026898971306483035	0.006148336298624694
28	0.0	0.0	0.0	0.006916878335952781	0.006148336298624694
29	0.0	0.0	0.0	0.019213550933202168	0.006148336298624694
30	0.0	0.0	0.0	0.03535293371709199	0.006148336298624694
31	0.0	0.0	0.0	0.09453067059135467	0.006148336298624694
32	0.0	0.0	0.0	0.17484331349213975	0.006148336298624694
33	0.0	0.0	0.0	0.2497761621316282	0.006148336298624694
34	0.0	0.0	0.0	0.3297045340137492	0.006148336298624694
35	0.0	0.0	0.0	0.42154530747445557	0.006148336298624694
36	0.0	0.0	0.0	0.5437434914096214	0.006148336298624694
37	0.0	0.0	0.0	0.7343419166669869	0.006148336298624694
38	0.0	0.0	0.0	1.0060215268624655	0.006148336298624694
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTATA	20	7.8496337E-4	43.995003	2
CCGCGCG	25	0.0023485047	35.20277	21
GGTATCA	75	6.002665E-11	32.263004	1
GTATCAA	295	0.0	32.064156	1
TAGGACG	50	2.3541452E-6	30.796503	4
GAATTAC	30	0.005740022	29.330002	1
TCTACAC	55	1.5901611E-4	23.997274	3
TGGGGGG	65	1.8052062E-5	23.69417	19
TAGAGTG	50	0.0025767474	21.997503	5
TATAACC	50	0.0025767474	21.997503	3
CTAGGAC	60	2.866597E-4	21.997501	3
TATCAAC	435	0.0	21.238966	2
AGGATAC	65	4.921638E-4	20.305387	5
GTCTAAG	55	0.0044772667	19.997728	1
CCTTTAC	55	0.0044772667	19.997728	1
TCTCCCG	55	0.0044772667	19.997728	10
ATCAACG	470	0.0	19.657343	3
CAACGCA	480	0.0	19.247814	5
TCAACGC	480	0.0	19.247814	4
CTGTAGG	70	8.1061316E-4	18.855003	1
>>END_MODULE