FastQCFastQC Report
Thu 2 Feb 2017
SRR4064027_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064027_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196459
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28130.23511043838526854No Hit
TATCAACGCAGAGTACTTTTTTTTT18180.15194837432791264No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14040.11734626928294242No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGT402.760076E-416.6252946
TTACGCA456.766245E-414.7761844
CGGTCCA1900.014.00083210
AAGACCG500.00149946813.2996775
CTTACGC655.4688153E-513.1491283
CGTCGTA2200.012.95531610
CCGTCGT2200.012.9547749
ACGGTAT1750.012.4859349
GACGTGA1301.9826984E-1012.4232957
CAAGACG2250.012.2431244
CGTCTTA701.08903834E-412.21603415
CCTATAT701.0958632E-412.2078622
GCGTTAT1251.4060788E-912.161741
TCGGCGT1103.8091457E-812.09112213
GTATCAA17250.012.0625951
ATAATAG951.0436088E-611.9956943
CCGATAA1209.998985E-911.8752099
AAGACGG2400.011.8747125
CGAACGA1052.7126043E-711.76358916
AACGAAC1052.7126043E-711.76358914
Produced by FastQC (version 0.11.5)