##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064027_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1196459
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255768062257044	32.0	32.0	32.0	32.0	32.0
2	30.847997298695567	32.0	32.0	32.0	32.0	32.0
3	30.868123353997085	32.0	32.0	32.0	32.0	32.0
4	30.90110400774285	32.0	32.0	32.0	32.0	32.0
5	30.800483760830918	32.0	32.0	32.0	32.0	32.0
6	34.46339155792217	36.0	36.0	36.0	32.0	36.0
7	34.39933253040848	36.0	36.0	36.0	32.0	36.0
8	34.36542831806188	36.0	36.0	36.0	32.0	36.0
9	34.50359268474724	36.0	36.0	36.0	32.0	36.0
10	34.19741002407939	36.0	36.0	36.0	32.0	36.0
11	34.4751679748324	36.0	36.0	36.0	32.0	36.0
12	34.28919419721027	36.0	36.0	36.0	32.0	36.0
13	34.379783176857714	36.0	36.0	36.0	32.0	36.0
14	34.27808892740997	36.0	36.0	36.0	32.0	36.0
15	34.21791386081763	36.0	36.0	36.0	32.0	36.0
16	34.21532288193745	36.0	36.0	36.0	32.0	36.0
17	34.13700176938784	36.0	36.0	36.0	32.0	36.0
18	34.13352317129129	36.0	36.0	36.0	32.0	36.0
19	34.14703805145015	36.0	36.0	36.0	32.0	36.0
20	34.124776528071585	36.0	36.0	36.0	32.0	36.0
21	34.10525642750817	36.0	36.0	36.0	32.0	36.0
22	34.097542832641984	36.0	36.0	36.0	32.0	36.0
23	34.04141805109912	36.0	36.0	36.0	32.0	36.0
24	34.011799819300116	36.0	36.0	36.0	32.0	36.0
25	33.60676128475777	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	7.0
5	12.0
6	51.0
7	25.0
8	62.0
9	75.0
10	105.0
11	42.0
12	60.0
13	54.0
14	112.0
15	187.0
16	357.0
17	409.0
18	574.0
19	793.0
20	1160.0
21	1681.0
22	2652.0
23	4060.0
24	6110.0
25	9189.0
26	13280.0
27	17298.0
28	23805.0
29	32350.0
30	43084.0
31	58596.0
32	82654.0
33	116564.0
34	249037.0
35	532010.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.77046789859926	16.93501793885822	11.0026111887305	26.291902973812025
2	16.85577423773977	19.63166442245085	37.35578719317558	26.156774146633804
3	19.052204082280834	23.686539806876908	28.296636936776554	28.96461917406571
4	12.596402747211874	15.177785597448374	35.96249789997267	36.26331375536708
5	14.60319423976943	36.44579597288733	33.72424751805714	15.226762269286098
6	34.353456887234145	35.36533274039052	16.88783600673079	13.393374365644537
7	30.33811659567709	30.159214502773402	20.951775854069396	18.550893047480113
8	28.084138287385528	32.95909554183796	19.440131568496135	19.516634602280376
9	27.62141365726475	13.781189088303197	18.291968080703008	40.305429173729046
10	15.70137975385372	26.57048502541828	31.894567036267986	25.83356818446002
11	37.60603708956622	20.754586020498277	22.48479689047071	19.154579999464797
12	24.794224056173782	23.405602324293856	29.073493917462145	22.72667970207022
13	29.85681155008334	19.265499537090083	25.16632558248453	25.711363330342053
14	23.637861459008512	19.42802182867418	25.268655780207833	31.66546093210948
15	25.19355923188471	27.13299848297063	22.240286077952227	25.433156207192425
16	25.73449897505936	25.4267240622274	23.896798388196164	24.941978574517073
17	24.04749838176335	25.59414792104678	25.24390631455175	25.114447382638115
18	24.74787733501513	24.84624218664396	26.23280183413645	24.173078644204463
19	25.67712245331531	24.650753529267114	25.31227678086007	24.359847236557503
20	25.836709839098766	24.319958913138763	24.85094302376547	24.992388223997004
21	26.843266997384536	24.06319605279776	24.403950882457984	24.68958606735972
22	26.02203026757014	24.270348805599493	24.76058875634966	24.947032170480714
23	24.573837615381077	24.141966298478874	25.41298792550366	25.87120816063639
24	24.965831739929772	24.810088042865484	25.221194525717785	25.00288569148696
25	24.889169937199085	24.447522592603747	25.347546347877582	25.315761122319586
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	63.0
1	63.0
2	251.5
3	440.0
4	440.0
5	440.0
6	1219.5
7	1999.0
8	1999.0
9	1999.0
10	2139.0
11	2279.0
12	2279.0
13	2279.0
14	2614.5
15	2950.0
16	2950.0
17	2950.0
18	5106.0
19	7262.0
20	7262.0
21	7262.0
22	11805.0
23	16348.0
24	16348.0
25	16348.0
26	24509.0
27	32670.0
28	32670.0
29	32670.0
30	41806.0
31	50942.0
32	50942.0
33	50942.0
34	62290.5
35	73639.0
36	73639.0
37	73639.0
38	85055.0
39	96471.0
40	96471.0
41	96471.0
42	110685.0
43	124899.0
44	124899.0
45	124899.0
46	139970.5
47	155042.0
48	155042.0
49	155042.0
50	159205.0
51	163368.0
52	163368.0
53	163368.0
54	151982.0
55	140596.0
56	140596.0
57	140596.0
58	129338.5
59	118081.0
60	118081.0
61	118081.0
62	103625.0
63	89169.0
64	89169.0
65	89169.0
66	73658.5
67	58148.0
68	58148.0
69	58148.0
70	43936.0
71	29724.0
72	29724.0
73	29724.0
74	22726.5
75	15729.0
76	15729.0
77	15729.0
78	12520.0
79	9311.0
80	9311.0
81	9311.0
82	6445.0
83	3579.0
84	3579.0
85	3579.0
86	2751.5
87	1924.0
88	1924.0
89	1924.0
90	1360.5
91	797.0
92	797.0
93	797.0
94	560.5
95	324.0
96	324.0
97	324.0
98	514.5
99	705.0
100	705.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0377781436722863
2	0.00417899819383698
3	0.0016715992775347924
4	0.0038446783383300222
5	0.009862435737455275
6	0.013874274003538776
7	0.022900910102226654
8	0.03577222453924456
9	0.047473419481988105
10	0.05566425594190858
11	0.054410556483757484
12	0.06193275323266405
13	0.06368793247407557
14	0.06552669167936385
15	0.05858955467759446
16	0.0651923718238569
17	0.059592514244115345
18	0.07605776712783305
19	0.07396826803091455
20	0.07881590593576546
21	0.07338320828377738
22	0.07706072669435392
23	0.08057108517717698
24	0.07563986730844935
25	0.07814726622475153
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1196459.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.96281481753707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.02869462237129	42.886766929835446
2	13.208705068781548	14.519752213498075
3	3.8931942240372512	6.419427395533932
4	1.6004461997372883	3.518601124063662
5	0.8556465991555061	2.351437278931973
6	0.5291633470416197	1.745058423100599
7	0.3412874250914559	1.3130682281399024
8	0.2447346738916641	1.0761045248430288
9	0.16636999612045883	0.8229746959166827
>10	0.965635404187095	9.583531347195752
>50	0.08240075884474127	3.185771275522259
>100	0.07455507745818281	8.454980440712268
>500	0.007180505904215233	2.71241142307088
>1k	0.00198609737776166	1.4101146996356042
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2813	0.23511043838526854	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1818	0.15194837432791264	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1404	0.11734626928294242	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	8.357996387673961E-5	8.357996387673961E-5
6	0.0	0.0	0.0	8.357996387673961E-5	8.357996387673961E-5
7	0.0	0.0	0.0	8.357996387673961E-5	8.357996387673961E-5
8	0.0	0.0	0.0	8.357996387673961E-5	8.357996387673961E-5
9	0.0	0.0	0.0	8.357996387673961E-5	8.357996387673961E-5
10	0.0	0.0	0.0	8.357996387673961E-5	8.357996387673961E-5
11	0.0	0.0	0.0	8.357996387673961E-5	8.357996387673961E-5
12	0.0	0.0	0.0	8.357996387673961E-5	8.357996387673961E-5
13	0.0	0.0	0.0	8.357996387673961E-5	8.357996387673961E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAGCGT	40	2.760076E-4	16.625294	6
TTACGCA	45	6.766245E-4	14.776184	4
CGGTCCA	190	0.0	14.000832	10
AAGACCG	50	0.001499468	13.299677	5
CTTACGC	65	5.4688153E-5	13.149128	3
CGTCGTA	220	0.0	12.955316	10
CCGTCGT	220	0.0	12.954774	9
ACGGTAT	175	0.0	12.485934	9
GACGTGA	130	1.9826984E-10	12.423295	7
CAAGACG	225	0.0	12.243124	4
CGTCTTA	70	1.08903834E-4	12.216034	15
CCTATAT	70	1.0958632E-4	12.207862	2
GCGTTAT	125	1.4060788E-9	12.16174	1
TCGGCGT	110	3.8091457E-8	12.091122	13
GTATCAA	1725	0.0	12.062595	1
ATAATAG	95	1.0436088E-6	11.995694	3
CCGATAA	120	9.998985E-9	11.875209	9
AAGACGG	240	0.0	11.874712	5
CGAACGA	105	2.7126043E-7	11.763589	16
AACGAAC	105	2.7126043E-7	11.763589	14
>>END_MODULE