FastQCFastQC Report
Thu 2 Feb 2017
SRR4064027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196459
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12130.10138249618248515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA2650.023.245571
GTATCAA17850.021.4456441
ACCGTCG3200.020.6235318
TACCGTC3200.020.6218077
ATACCGT3250.020.3045486
CCGTCGT3300.018.6668979
CGTCGTA3550.018.5917810
TAGGACG3150.018.155924
TACGCTC750.001289668417.60242534
GTATATG1651.6589183E-917.3335481
TATGTAC1551.441731E-817.0296213
GTAAACG2850.016.98479727
CGAGCCG4750.016.67319315
GGCGTTA3300.016.66617642
AACGCAG22600.016.643446
TAAACGC3050.016.59175528
CTATACT1602.1331289E-816.4974464
GCATTCG4150.016.4332522
AATCCCG1351.0862295E-616.29513519
GTCGTAG3800.016.21072611
Produced by FastQC (version 0.11.5)