##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064027_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1196459
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.317474313787603	32.0	32.0	32.0	32.0	32.0
2	31.386600794511136	32.0	32.0	32.0	32.0	32.0
3	31.466611058130702	32.0	32.0	32.0	32.0	32.0
4	31.548539481921235	32.0	32.0	32.0	32.0	32.0
5	31.48959220499825	32.0	32.0	32.0	32.0	32.0
6	35.07147006291064	36.0	36.0	36.0	36.0	36.0
7	35.09599576751063	36.0	36.0	36.0	36.0	36.0
8	35.045377233987956	36.0	36.0	36.0	36.0	36.0
9	35.15726238843119	36.0	36.0	36.0	36.0	36.0
10	34.998212224572676	36.0	36.0	36.0	36.0	36.0
11	35.14593061692879	36.0	36.0	36.0	36.0	36.0
12	35.05346693869159	36.0	36.0	36.0	36.0	36.0
13	35.096891744723386	36.0	36.0	36.0	36.0	36.0
14	35.04697026809945	36.0	36.0	36.0	36.0	36.0
15	35.017313589517066	36.0	36.0	36.0	36.0	36.0
16	35.021227639225415	36.0	36.0	36.0	36.0	36.0
17	34.98842083180452	36.0	36.0	36.0	36.0	36.0
18	34.98005782061901	36.0	36.0	36.0	36.0	36.0
19	34.98714289415684	36.0	36.0	36.0	36.0	36.0
20	34.9797945437328	36.0	36.0	36.0	36.0	36.0
21	34.96809251299042	36.0	36.0	36.0	36.0	36.0
22	34.95112160132524	36.0	36.0	36.0	36.0	36.0
23	34.897008589512886	36.0	36.0	36.0	32.0	36.0
24	34.870709318079435	36.0	36.0	36.0	32.0	36.0
25	34.84121311302769	36.0	36.0	36.0	32.0	36.0
26	34.781396604480385	36.0	36.0	36.0	32.0	36.0
27	34.77952692068847	36.0	36.0	36.0	32.0	36.0
28	34.746443463587134	36.0	36.0	36.0	32.0	36.0
29	34.717130298656286	36.0	36.0	36.0	32.0	36.0
30	34.702187872714404	36.0	36.0	36.0	32.0	36.0
31	34.68870140974325	36.0	36.0	36.0	32.0	36.0
32	34.65910240133594	36.0	36.0	36.0	32.0	36.0
33	34.62792038841281	36.0	36.0	36.0	32.0	36.0
34	34.62072833252122	36.0	36.0	36.0	32.0	36.0
35	34.579411413178384	36.0	36.0	36.0	32.0	36.0
36	34.55045179149474	36.0	36.0	36.0	32.0	36.0
37	34.5371166082582	36.0	36.0	36.0	32.0	36.0
38	34.4972339210955	36.0	36.0	36.0	32.0	36.0
39	34.495412713682626	36.0	36.0	36.0	32.0	36.0
40	34.473301634239036	36.0	36.0	36.0	32.0	36.0
41	34.46127280583789	36.0	36.0	36.0	32.0	36.0
42	34.39552713465317	36.0	36.0	36.0	32.0	36.0
43	34.38089562617691	36.0	36.0	36.0	32.0	36.0
44	34.31067759112514	36.0	36.0	36.0	32.0	36.0
45	34.28738970579017	36.0	36.0	36.0	32.0	36.0
46	34.25541117581129	36.0	36.0	36.0	32.0	36.0
47	34.21563463520271	36.0	36.0	36.0	32.0	36.0
48	34.171603038633165	36.0	36.0	36.0	32.0	36.0
49	34.1523470507556	36.0	36.0	36.0	32.0	36.0
50	33.55083542352893	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	4.0
20	11.0
21	27.0
22	131.0
23	387.0
24	916.0
25	2270.0
26	4563.0
27	8529.0
28	14298.0
29	21617.0
30	31216.0
31	43921.0
32	63901.0
33	101518.0
34	211112.0
35	692036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.884151830679585	17.84364057945817	11.524299980189893	26.74790760967235
2	16.020075974441163	20.55839960550125	36.9062772941732	26.515247125884382
3	18.3658766124678	24.277659558463398	28.682745236355977	28.67371859271282
4	12.214793820766111	15.875094758784044	36.04461164151885	35.865499778930996
5	14.138553849317026	37.00895726472867	33.49943458154437	15.35305430440993
6	34.376329446022716	35.82134585697606	16.595163888046123	13.207160808955107
7	30.163674643259817	30.326989892674966	20.931598993362915	18.5777364707023
8	27.93269801363692	33.346009638449495	19.316181596010892	19.405110751902697
9	27.701779357645957	13.61627758280169	18.13182909674761	40.550113962804744
10	15.47204725988927	26.945675867233675	31.935696327796947	25.646580545080106
11	37.8568759982582	20.72691166182878	22.397173659941544	19.019038679971484
12	24.762966628108344	23.49697774092821	28.9937950079234	22.746260623040047
13	29.84962230056484	19.29493319425569	25.143549187850095	25.711895317329375
14	23.51824641816568	19.436183923493925	25.29884140969899	31.746728248641404
15	25.136367207677683	27.291662835735814	22.163787756957944	25.408182199628556
16	25.698732586012646	25.618578931708086	23.799199633583296	24.88348884869598
17	24.075459334586473	25.71956080400582	25.191586172196455	25.01339368921125
18	24.810983121791562	24.750805296317285	26.32654494029858	24.11166664159257
19	25.777565298936278	24.569834820917393	25.408559758420473	24.24404012172586
20	25.931609401471515	24.251640894612482	24.89220202080988	24.92454768310612
21	26.855997572837847	24.09869456454421	24.41345670850401	24.63185115411393
22	26.00444478990887	24.25059989619351	24.908124571129594	24.836830742768022
23	24.612333288478528	24.021754302091264	25.482488656052517	25.883423753377695
24	25.077771481394183	24.64541052142414	25.313217223562294	24.96360077361938
25	25.1122504245958	24.290306511360445	25.394757077711063	25.202685986332696
26	24.65226843597903	25.227161218224385	25.746303275584943	24.374267070211644
27	25.30757445186593	24.663881034155622	24.944561789469372	25.08398272450908
28	24.625818385153597	24.752363527249415	25.559527620525053	25.062290467071936
29	24.685898357443993	24.72969539328735	25.35137927230055	25.23302697696811
30	24.597235583854822	24.933238271876323	25.608670541006116	24.860855603262735
31	25.13152373612669	24.62707775740119	24.902914000946204	25.338484505525923
32	25.031176551221485	24.814528774368696	24.741309849784187	25.412984824625628
33	24.59227955680671	24.472922280693542	25.262201523226192	25.67259663927356
34	25.201600770295478	24.644941926942288	25.492634718126684	24.660822584635547
35	25.740771852048734	24.486698244179934	25.347766379894704	24.424763523876628
36	24.672777987209525	25.0522627405319	25.0479162191282	25.227043053130373
37	25.619563927295587	24.931670352706355	24.712263228368965	24.736502491629096
38	24.89428375835762	24.68113774832555	25.252034708529102	25.172543784787727
39	25.488588322024462	24.54810490753139	24.698639090743445	25.264667679700704
40	25.758524282556095	24.720273144801013	25.140605214658546	24.380597357984346
41	24.650959860383946	25.046304654537188	25.575548467767277	24.72718701731159
42	25.811070137841806	25.28626496518015	24.90948176429486	23.993183132683185
43	25.024008665686537	24.524611703190207	25.275838464515232	25.175541166608024
44	24.70566995919582	25.066237841072642	24.808725174391487	25.41936702534005
45	24.851267838247608	25.11588487905012	24.919636991556658	25.11321029114561
46	24.662981873861735	24.90193898778803	24.923837121348257	25.51124201700198
47	25.071943017285548	24.79553807024714	25.17215757171526	24.96036134075205
48	25.685786786756665	25.132727534109637	24.40222460708304	24.779261072050655
49	25.01182661431196	25.346398607886307	24.600695053884227	25.04107972391751
50	25.058715562591203	25.53479220959408	24.368421140611442	25.038071087203267
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	22.0
2	27.0
3	71.5
4	116.0
5	153.0
6	190.0
7	185.0
8	180.0
9	236.0
10	292.0
11	462.0
12	632.0
13	1192.5
14	1753.0
15	2361.0
16	2969.0
17	3154.5
18	3340.0
19	3466.0
20	3592.0
21	3914.0
22	4236.0
23	4777.0
24	5318.0
25	6180.5
26	7043.0
27	9020.0
28	10997.0
29	12869.5
30	14742.0
31	16705.0
32	18668.0
33	21965.5
34	25263.0
35	29212.0
36	33161.0
37	38649.0
38	44137.0
39	47520.0
40	50903.0
41	55482.5
42	60062.0
43	61938.5
44	63815.0
45	70336.0
46	76857.0
47	81809.0
48	86761.0
49	89905.0
50	93049.0
51	91211.0
52	89373.0
53	88347.5
54	87322.0
55	86866.5
56	86411.0
57	84148.0
58	81885.0
59	74908.5
60	67932.0
61	59999.5
62	52067.0
63	45204.0
64	38341.0
65	32319.0
66	26297.0
67	22557.5
68	18818.0
69	16851.0
70	14884.0
71	11662.0
72	8440.0
73	7317.5
74	6195.0
75	4894.5
76	3594.0
77	3110.0
78	2626.0
79	2167.5
80	1709.0
81	1397.0
82	1085.0
83	904.0
84	723.0
85	521.0
86	319.0
87	228.0
88	137.0
89	105.5
90	74.0
91	59.0
92	44.0
93	34.0
94	24.0
95	22.5
96	21.0
97	12.5
98	4.0
99	9.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00835799638767396
2	3.343198555069585E-4
3	8.357996387673962E-5
4	0.0
5	0.0
6	1.6715992775347924E-4
7	0.0
8	8.357996387673962E-5
9	0.001337279422027834
10	9.193796026441358E-4
11	0.0
12	2.5073989163021886E-4
13	4.178998193836981E-4
14	4.178998193836981E-4
15	0.0047640579409741575
16	5.850597471371773E-4
17	0.0
18	4.178998193836981E-4
19	0.0
20	6.68639711013917E-4
21	0.0
22	1.6715992775347924E-4
23	5.014797832604377E-4
24	4.178998193836981E-4
25	0.0022566590246719696
26	0.005181957760357856
27	0.006853557037892649
28	0.004011838266083502
29	0.002590978880178928
30	0.003343198555069585
31	0.008107256496043743
32	0.003928258302206762
33	0.00426257815771372
34	0.0029252987356858866
35	0.0031760386273161053
36	0.00835799638767396
37	0.0044297380854672
38	0.008023676532167004
39	0.0044297380854672
40	0.002005919133041751
41	0.0029252987356858866
42	0.0012536994581510941
43	0.001337279422027834
44	0.001086539530397615
45	0.001086539530397615
46	8.357996387673962E-4
47	0.00217307906079523
48	0.0019223391691650111
49	4.178998193836981E-4
50	0.001086539530397615
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1196459.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.302121682694775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.85870447188391	45.97629783859442
2	12.96461056500926	15.1172860545984
3	3.757921338692621	6.572843614873573
4	1.5074673871835091	3.515541881610676
5	0.7836757454682118	2.2844979336032107
6	0.46548909360563057	1.6283401066417662
7	0.31238458107702455	1.2748878700425221
8	0.2188937376573902	1.0209575462784826
9	0.158289714155935	0.8305763558254466
>10	0.8348312944555276	8.762839990231884
>50	0.07005237964061045	2.836012343221348
>100	0.06060868396222463	7.173403665596631
>500	0.00634947586035881	2.5275421180356625
>1k	7.215313477680466E-4	0.47897268084604977
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1213	0.10138249618248515	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	8.357996387673961E-5	8.357996387673961E-5
6	0.0	0.0	0.0	8.357996387673961E-5	8.357996387673961E-5
7	0.0	0.0	0.0	8.357996387673961E-5	8.357996387673961E-5
8	0.0	0.0	0.0	8.357996387673961E-5	8.357996387673961E-5
9	0.0	0.0	0.0	8.357996387673961E-5	8.357996387673961E-5
10	0.0	0.0	0.0	8.357996387673961E-5	8.357996387673961E-5
11	0.0	0.0	0.0	8.357996387673961E-5	8.357996387673961E-5
12	0.0	0.0	0.0	8.357996387673961E-5	4.1789981938369805E-4
13	0.0	0.0	0.0	8.357996387673961E-5	5.850597471371773E-4
14	0.0	0.0	0.0	8.357996387673961E-5	5.850597471371773E-4
15	0.0	0.0	0.0	8.357996387673961E-5	5.850597471371773E-4
16	0.0	0.0	0.0	8.357996387673961E-5	5.850597471371773E-4
17	0.0	0.0	0.0	8.357996387673961E-5	5.850597471371773E-4
18	0.0	0.0	0.0	1.6715992775347921E-4	5.850597471371773E-4
19	0.0	0.0	0.0	1.6715992775347921E-4	5.850597471371773E-4
20	0.0	0.0	0.0	3.3431985550695843E-4	5.850597471371773E-4
21	0.0	0.0	0.0	4.1789981938369805E-4	6.686397110139169E-4
22	0.0	0.0	0.0	4.1789981938369805E-4	6.686397110139169E-4
23	0.0	0.0	0.0	5.850597471371773E-4	6.686397110139169E-4
24	0.0	0.0	0.0	0.001086539530397615	6.686397110139169E-4
25	0.0	0.0	0.0	0.001504439349781313	6.686397110139169E-4
26	0.0	0.0	0.0	0.0017551792414115318	6.686397110139169E-4
27	0.0	0.0	0.0	0.0020894990969184905	6.686397110139169E-4
28	0.0	0.0	0.0	0.003426778518946324	7.522196748906565E-4
29	0.0	0.0	0.0	0.007104296929522867	7.522196748906565E-4
30	0.0	0.0	0.0	0.013957853967415515	7.522196748906565E-4
31	0.0	0.0	0.0	0.030172366959503	7.522196748906565E-4
32	0.0	0.0	0.0	0.04438096081854873	7.522196748906565E-4
33	0.0	0.0	0.0	0.0595089342802386	7.522196748906565E-4
34	0.0	0.0	0.0	0.07589060720007956	8.357996387673961E-4
35	0.0	0.0	0.0	0.09586621856662034	8.357996387673961E-4
36	0.0	0.0	0.0	0.12562068570673965	8.357996387673961E-4
37	0.0	0.0	0.0	0.1677449875006164	8.357996387673961E-4
38	0.0	0.0	0.0	0.22909268098614327	8.357996387673961E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAA	265	0.0	23.24557	1
GTATCAA	1785	0.0	21.445644	1
ACCGTCG	320	0.0	20.623531	8
TACCGTC	320	0.0	20.621807	7
ATACCGT	325	0.0	20.304548	6
CCGTCGT	330	0.0	18.666897	9
CGTCGTA	355	0.0	18.59178	10
TAGGACG	315	0.0	18.15592	4
TACGCTC	75	0.0012896684	17.602425	34
GTATATG	165	1.6589183E-9	17.333548	1
TATGTAC	155	1.441731E-8	17.029621	3
GTAAACG	285	0.0	16.984797	27
CGAGCCG	475	0.0	16.673193	15
GGCGTTA	330	0.0	16.666176	42
AACGCAG	2260	0.0	16.64344	6
TAAACGC	305	0.0	16.591755	28
CTATACT	160	2.1331289E-8	16.497446	4
GCATTCG	415	0.0	16.43325	22
AATCCCG	135	1.0862295E-6	16.295135	19
GTCGTAG	380	0.0	16.210726	11
>>END_MODULE