FastQCFastQC Report
Thu 2 Feb 2017
SRR4064026_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064026_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661767
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT18810.2842390146380826No Hit
TATCAACGCAGAGTACTTTTTTTTT14720.2224347844483028No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14360.2169948033069041No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9050.13675508147127313No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7560.11423960396937291No Hit
GGTATCAACGCAGAGTACTTTTTTT6840.10335964168657549No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAACA350.002170849616.2829914
AATCGAA456.7520037E-414.778667
GTATTAG1051.36788E-913.569161
TTAACCA852.696579E-713.4095234
CGCATCG1001.0022632E-813.30079513
TTAGACC500.00149984713.2977774
GGTTCCG655.4402015E-513.1546318
TTTATAC655.4595403E-513.1496583
TCTTACA802.0049993E-613.0563662
GCTCGTA604.084442E-412.6674249
CCGACCA751.4746369E-512.6674229
CGACCAT905.390284E-712.66646510
GCGTTAT604.0922686E-412.6645491
TTATACA604.0922686E-412.6645494
AAGCTCG701.0884661E-412.2150157
GTATTAA701.09077446E-412.2122441
TTATACT701.09077446E-412.2122444
GCATTAA701.09077446E-412.2122441
CTAAGGC701.0923158E-412.2103973
GATCTGT550.003065605412.0907166