##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064026_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	661767
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.130689502498612	32.0	32.0	32.0	32.0	32.0
2	30.6677682628478	32.0	32.0	32.0	32.0	32.0
3	30.68231870129517	32.0	32.0	32.0	32.0	32.0
4	30.708971586676277	32.0	32.0	32.0	32.0	32.0
5	30.609960907691075	32.0	32.0	32.0	32.0	32.0
6	34.236587802051176	36.0	36.0	36.0	32.0	36.0
7	34.161558373264306	36.0	36.0	36.0	32.0	36.0
8	34.09666090935329	36.0	36.0	36.0	32.0	36.0
9	34.264886281727556	36.0	36.0	36.0	32.0	36.0
10	33.9093865363489	36.0	36.0	36.0	32.0	36.0
11	34.274713003217144	36.0	36.0	36.0	32.0	36.0
12	34.040861813901266	36.0	36.0	36.0	32.0	36.0
13	34.13685783667061	36.0	36.0	36.0	32.0	36.0
14	34.02576435512801	36.0	36.0	36.0	32.0	36.0
15	33.95384780443872	36.0	36.0	36.0	32.0	36.0
16	33.96467790022773	36.0	36.0	36.0	32.0	36.0
17	33.87327866152286	36.0	36.0	36.0	32.0	36.0
18	33.878141400220926	36.0	36.0	36.0	32.0	36.0
19	33.893405080640164	36.0	36.0	36.0	32.0	36.0
20	33.876432339479	36.0	36.0	36.0	32.0	36.0
21	33.84914025631378	36.0	36.0	36.0	32.0	36.0
22	33.825045673175	36.0	36.0	36.0	32.0	36.0
23	33.77832983512324	36.0	36.0	36.0	32.0	36.0
24	33.76607929981398	36.0	36.0	36.0	32.0	36.0
25	33.34735035140767	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	7.0
5	9.0
6	20.0
7	9.0
8	42.0
9	30.0
10	65.0
11	15.0
12	39.0
13	45.0
14	54.0
15	122.0
16	211.0
17	253.0
18	319.0
19	503.0
20	817.0
21	1162.0
22	1810.0
23	2911.0
24	4384.0
25	6331.0
26	8875.0
27	11710.0
28	15961.0
29	20841.0
30	27087.0
31	36059.0
32	49829.0
33	68724.0
34	139204.0
35	264318.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.30768602812456	17.418509235267262	11.271893887398978	26.001910849209203
2	16.445224897389245	20.22882651981793	37.764838512733405	25.561110070059424
3	18.710644541727554	24.36362592311075	28.931616892605593	27.994112642556107
4	12.371193220369719	15.36493554183094	36.59087234245112	35.67299889534822
5	14.192623130431892	36.98544846210691	34.06199326902588	14.759935138435312
6	33.49418457037153	36.00760479259107	17.208110166725103	13.290100470312291
7	29.53902424279064	30.859682002297323	21.34438667553352	18.256907079378514
8	27.79689968180547	33.11354480798736	20.025848581729132	19.063706928478034
9	27.677401115423166	13.83227530905446	18.817512355375214	39.67281122014716
10	15.414226143858048	26.98495015134517	32.39920653399899	25.201617170797793
11	36.82044552225041	21.111961126414748	23.09510058570422	18.972492765630623
12	24.389838247790234	23.861701709450816	29.593033980362332	22.155426062396618
13	29.607302373543565	19.581809989506695	25.534266542077553	25.276621094872194
14	23.282795368873714	19.671097495883426	26.07072492519101	30.975382210051848
15	24.91559379181566	27.449518812794366	22.898765469431574	24.736121925958404
16	25.055001217209067	25.78744354342412	24.623459020880052	24.53409621848676
17	23.525747351327322	25.89840164870141	25.89885525327011	24.67699574670116
18	24.16797725623034	25.16740261311396	26.860936365835954	23.803683764819745
19	25.196281241683	24.990926907164116	26.02223512617648	23.790556724976412
20	25.452095563883166	24.497848028140474	25.704797248863525	24.345259159112835
21	26.420957603013473	24.395090254454505	25.011605980368877	24.172346162163144
22	25.408944841404864	24.555895807341876	25.640013610071456	24.39514574118181
23	24.16812477032032	24.549827825305822	25.8138033140415	25.46824409033236
24	24.250274091716758	25.172432044157123	25.932025254243698	24.64526860988242
25	24.510600073495052	24.77569642611403	25.920011372073997	24.79369212831692
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	193.0
1	193.0
2	299.0
3	405.0
4	405.0
5	405.0
6	1166.5
7	1928.0
8	1928.0
9	1928.0
10	1854.5
11	1781.0
12	1781.0
13	1781.0
14	1927.5
15	2074.0
16	2074.0
17	2074.0
18	3526.0
19	4978.0
20	4978.0
21	4978.0
22	7852.0
23	10726.0
24	10726.0
25	10726.0
26	15880.5
27	21035.0
28	21035.0
29	21035.0
30	26450.0
31	31865.0
32	31865.0
33	31865.0
34	38384.0
35	44903.0
36	44903.0
37	44903.0
38	50442.0
39	55981.0
40	55981.0
41	55981.0
42	63182.5
43	70384.0
44	70384.0
45	70384.0
46	77833.0
47	85282.0
48	85282.0
49	85282.0
50	86969.0
51	88656.0
52	88656.0
53	88656.0
54	81466.0
55	74276.0
56	74276.0
57	74276.0
58	67461.0
59	60646.0
60	60646.0
61	60646.0
62	53246.0
63	45846.0
64	45846.0
65	45846.0
66	37546.5
67	29247.0
68	29247.0
69	29247.0
70	22182.0
71	15117.0
72	15117.0
73	15117.0
74	11593.0
75	8069.0
76	8069.0
77	8069.0
78	6388.5
79	4708.0
80	4708.0
81	4708.0
82	3242.5
83	1777.0
84	1777.0
85	1777.0
86	1350.5
87	924.0
88	924.0
89	924.0
90	683.0
91	442.0
92	442.0
93	442.0
94	306.5
95	171.0
96	171.0
97	171.0
98	262.0
99	353.0
100	353.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04246207502036215
2	0.006497755252226237
3	0.0015111058726107528
4	0.0030222117452215055
5	0.007555529363053764
6	0.011937736393624948
7	0.01919104458215656
8	0.03354655037195871
9	0.04744872439997764
10	0.055155364350292474
11	0.05152871025602667
12	0.05817757609551398
13	0.05938646079360258
14	0.06286200430060732
15	0.05772424433373076
16	0.06316422547512947
17	0.06014201372990796
18	0.07359085599614365
19	0.07117308659996645
20	0.07751973126493161
21	0.07102197601270538
22	0.07434640893244902
23	0.07857750537575914
24	0.07434640893244902
25	0.0752530724560155
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	661767.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.02495163490991
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.7105977549131	49.8638527605315
2	11.463894627103834	13.991672303334301
3	2.9829483203122993	5.461028309294814
4	1.2100786274493063	2.9537995885812833
5	0.6849034354753692	2.0898099512234025
6	0.4005176882267333	1.4664943531777397
7	0.2700505191082249	1.153587390729322
8	0.19324417978316	0.9434173379996139
9	0.13932847082714925	0.7652261874233465
>10	0.7998977273344954	8.829555449476945
>50	0.07698799819635654	3.3141921702852204
>100	0.06382543153087548	7.332648613625005
>500	0.0029801757912944382	1.1089223468075438
>1k	7.450439478236096E-4	0.7257932375100898
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1881	0.2842390146380826	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1472	0.2224347844483028	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1436	0.2169948033069041	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	905	0.13675508147127313	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	756	0.11423960396937291	No Hit
GGTATCAACGCAGAGTACTTTTTTT	684	0.10335964168657549	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	1.5111058726107528E-4
9	0.0	0.0	0.0	0.0	1.5111058726107528E-4
10	0.0	0.0	0.0	0.0	1.5111058726107528E-4
11	0.0	0.0	0.0	0.0	1.5111058726107528E-4
12	0.0	0.0	0.0	1.5111058726107528E-4	1.5111058726107528E-4
13	0.0	0.0	0.0	1.5111058726107528E-4	1.5111058726107528E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAACA	35	0.0021708496	16.282991	4
AATCGAA	45	6.7520037E-4	14.77866	7
GTATTAG	105	1.36788E-9	13.56916	1
TTAACCA	85	2.696579E-7	13.409523	4
CGCATCG	100	1.0022632E-8	13.300795	13
TTAGACC	50	0.001499847	13.297777	4
GGTTCCG	65	5.4402015E-5	13.154631	8
TTTATAC	65	5.4595403E-5	13.149658	3
TCTTACA	80	2.0049993E-6	13.056366	2
GCTCGTA	60	4.084442E-4	12.667424	9
CCGACCA	75	1.4746369E-5	12.667422	9
CGACCAT	90	5.390284E-7	12.666465	10
GCGTTAT	60	4.0922686E-4	12.664549	1
TTATACA	60	4.0922686E-4	12.664549	4
AAGCTCG	70	1.0884661E-4	12.215015	7
GTATTAA	70	1.09077446E-4	12.212244	1
TTATACT	70	1.09077446E-4	12.212244	4
GCATTAA	70	1.09077446E-4	12.212244	1
CTAAGGC	70	1.0923158E-4	12.210397	3
GATCTGT	55	0.0030656054	12.090716	6
>>END_MODULE